公开(公告)号：US20130111631A1

公开(公告)日：2013-05-02

申请号：US13808530

申请日：2011-07-06

申请人： Gad Galili ,  Asaph Aharoni ,  Vered Tzin ,  Sergey Malitsky ,  Ilana Rogachev

发明人： Gad Galili ,  Asaph Aharoni ,  Vered Tzin ,  Sergey Malitsky ,  Ilana Rogachev

IPC分类号： C12N15/82

CPC分类号： C12N15/8251 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/8243 ,  C12N15/8254 ,  C12N15/8255 ,  C12Y205/01054

摘要： The present invention provides transgenic plants comprising 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthase that is insensitive to feedback inhibition, particularly to feedback inhibition by phenylalanine, producing increased amounts of the Shikimate pathway primary and secondary metabolites, including aromatic amino acids compared to corresponding non-transgenic plants.

摘要翻译： 本发明提供了包含对反馈抑制不敏感的3-脱氧-D-阿拉伯 - 七肽 - 七磷酸酯（DAHP）合成酶的转基因植物，特别是苯丙氨酸的反馈抑制，产生增加量的莽草酸通道初级和次级代谢物，包括 芳香族氨基酸与相应的非转基因植物相比。

公开(公告)号：US20100281567A1

公开(公告)日：2010-11-04

申请号：US12745853

申请日：2008-12-03

申请人： Gad Galili ,  Vered Tzin ,  Asaph Aharoni ,  Sergey Malitsky

发明人： Gad Galili ,  Vered Tzin ,  Asaph Aharoni ,  Sergey Malitsky

IPC分类号： A01H1/00 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C12N15/8251 ,  C12N15/8254

摘要： The present invention relates to means and methods for altering the level of at least one of the aromatic amino acids phenylalanine, tryptophan and tyrosine and secondary metabolites in plants. Particularly, the present invention discloses transgenic plants comprising polynucleotides encoding chorismate mutase and prephenate dehydratase enzymes, having elevated amounts of at least one of phenylalanine, tyrosine and modified amount of at least one secondary metabolite derived therefrom, and reduced amount of tryptophan and at least one secondary metabolite derived from tryptophan.

摘要翻译： 本发明涉及用于改变植物中至少一种芳香族氨基酸苯丙氨酸，色氨酸和酪氨酸和次级代谢物的水平的方法和方法。 特别地，本发明公开了转基因植物，其包含编码绒毛酸变位酶和预沉酸脱水酶的多核苷酸，其具有升高量的苯丙氨酸，酪氨酸和修饰量的至少一种由其衍生的至少一种次级代谢物，并且减少量的色氨酸和至少一种 衍生自色氨酸的次生代谢产物。

公开(公告)号：US09909135B2

公开(公告)日：2018-03-06

申请号：US13808530

申请日：2011-07-06

申请人： Gad Galili ,  Asaph Aharoni ,  Vered Tzin ,  Sergey Malitsky ,  Ilana Rogachev

发明人： Gad Galili ,  Asaph Aharoni ,  Vered Tzin ,  Sergey Malitsky ,  Ilana Rogachev

IPC分类号： C12N15/82 ,  C12N9/02 ,  A01H5/10 ,  C12N9/88 ,  C12N9/10

CPC分类号： C12N15/8251 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/8243 ,  C12N15/8254 ,  C12N15/8255 ,  C12Y205/01054

摘要： The present invention provides transgenic plants comprising 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthase that is insensitive to feedback inhibition, particularly to feedback inhibition by phenylalanine, producing increased amounts of the Shikimate pathway primary and secondary metabolites, including aromatic amino acids compared to corresponding non-transgenic plants.

公开(公告)号：US4886878A

公开(公告)日：1989-12-12

申请号：US274828

申请日：1988-11-22

申请人： Brian Larkins ,  Richard E. Cuellar ,  John C. Wallace ,  Gad Galili

发明人： Brian Larkins ,  Richard E. Cuellar ,  John C. Wallace ,  Gad Galili

IPC分类号： C07K14/425 ,  C12N15/82 ,  C12P21/02

CPC分类号： C07K14/425 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12P21/02

摘要： Modified 19kd and 22kd zeins containing lysine are provided. Lysine additions are made by modifying structural genes which encode 19kd and 22kd pre-zeins. Modified zeins produced from the modified structural gene templates are found to retain important properties of unmodified zeins, having similar molecular weights, having the ability to form protein bodies within the rough endoplasmic reticulum of a host cell and being soluble in alcohol.

摘要翻译： 提供了含有赖氨酸的19kd和22kd的玉米蛋白质。 通过修饰编码19kd和22kd前原蛋白的结构基因来制备赖氨酸添加物。 发现由修饰的结构基因模板产生的修饰的玉米醇溶蛋白保留了具有相似分子量的未修饰玉米蛋白的重要性质，具有在宿主细胞的粗糙内质网内形成蛋白质体并且可溶于醇的能力。

公开(公告)号：US06677506B1

公开(公告)日：2004-01-13

申请号：US09701068

申请日：2000-11-24

申请人： Gad Galili ,  Orit Shaul ,  Dirk Inze ,  Marc Van Montagu ,  Donald W. Hilgemann

发明人： Gad Galili ,  Orit Shaul ,  Dirk Inze ,  Marc Van Montagu ,  Donald W. Hilgemann

IPC分类号： A01H500

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： An isolated DNA molecule is provided coding for a polypeptide of the 11-12 transmembrane domain transporter family having a Mg2+/H+ or Zn2+/H+ exchange activity, herein designated MHX. The genomic MHX DNA was isolated from Arabidopsis thaliana cv. C-24. Transgenic plants transformed with said DNA and expressing MHX are shown to have a lower content of sodium as compared with corresponding wild-type plants or a higher dry matter weight upon growth in calcium-rich media as compared with corresponding wild-type plants. These transgenic plants are tolerant to stress conditions, particularly high salinity and calcium-rich media, e.g. saline and calcareous soils.

摘要翻译： 提供分离的DNA分子，其编码具有Mg 2+ / H 2+或Zn 2+ 2+ / H +交换活性的11-12跨膜结构域转运蛋白家族的多肽，这里称为MHX。 从拟南芥cv。中分离出基因组MHX DNA。 C-24。 与相应的野生型植物相比，用所述DNA转化并表达MHX的转基因植物显示与相应的野生型植物相比具有较低的钠含量或较富钙培养基生长时更高的干物质重量。 这些转基因植物耐受胁迫条件，特别是高盐度和富含钙的培养基，例如， 盐水和钙质土壤。

公开(公告)号：US5367110A

公开(公告)日：1994-11-22

申请号：US792466

申请日：1991-11-13

申请人： Gad Galili ,  Orit Shaul ,  Avihai Perl

发明人： Gad Galili ,  Orit Shaul ,  Avihai Perl

IPC分类号： C12N9/12 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12P13/08 ,  A01H1/04 ,  C12P21/04

CPC分类号： C12N15/8254 ,  C12N15/8251 ,  C12N15/8253 ,  C12N9/12 ,  C12N9/88 ,  C12P13/08

摘要： A chimeric gene construct comprising a DNA sequence encoding an enzyme having aspartate kinase (AK) activity is provided, which is capable of expression in plant cells with subsequent increased production of threonine. Transgenic plants containing in their cells said chimeric gene overproduce threonine and transgenic plants containing in their cells said chimeric gene and a second chimeric gene comprising a DNA sequence coding for an enzyme having dihydrodipicolinate synthase (DHPS) activity, overproduce both threonine and lysine. The transgenic plants are resistant to lysine and threonine, to derivatives thereof and to selective inhibitors of the plant enzymes DHPS or AK, and thus these compounds may be used as selective herbicides in locus where the transgenic plants are cultivated.

摘要翻译： 提供了包含编码具有天冬氨酸激酶（AK）活性的酶的DNA序列的嵌合基因构建体，其能够在随后增加的苏氨酸的产生中在植物细胞中表达。 在其细胞中含有转基因植物，所述嵌合基因过量产生苏氨酸和在其细胞中含有所述嵌合基因的转基因植物，以及包含编码具有二氢吡啶二羧酸合酶（DHPS）活性的酶的DNA序列的第二嵌合基因，其过量产生苏氨酸和赖氨酸。 转基因植物对赖氨酸和苏氨酸，其衍生物和植物酶DHPS或AK的选择性抑制剂具有抗性，因此这些化合物可用作培养转基因植物的基因座中的选择性除草剂。