公开(公告)号：US07176352B1

公开(公告)日：2007-02-13

申请号：US09529172

申请日：1998-10-08

Applicant: Meir Edelman ,  Avihai Perl ,  Moshe Flaishman ,  Amnon Blumenthal

Inventor： Meir Edelman ,  Avihai Perl ,  Moshe Flaishman ,  Amnon Blumenthal

IPC: C12N15/82 ,  A01H1/00 ,  C12N5/14

CPC classification number: C12N5/0025 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8257

Abstract: The present invention concerns genetically stable transformed Lemnaceae plants and methods for their transformation by Agrobacterium cells. The present invention further concerns a method for regeneration of plants from calli, utilizing low sucrose media and products of interest produce from said plants. The present invention further concerns booster media for use in the above methods.

Abstract translation: 本发明涉及遗传稳定的转化莲子科植物及其通过农杆菌细胞转化的方法。 本发明还涉及一种利用低蔗糖培养基和来自所述植物的感兴趣的产品从愈伤组织再生植物的方法。 本发明还涉及用于上述方法的增压介质。

公开(公告)号：US5367110A

公开(公告)日：1994-11-22

申请号：US792466

申请日：1991-11-13

Applicant: Gad Galili ,  Orit Shaul ,  Avihai Perl

Inventor： Gad Galili ,  Orit Shaul ,  Avihai Perl

IPC: C12N9/12 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12P13/08 ,  A01H1/04 ,  C12P21/04

CPC classification number: C12N15/8254 ,  C12N15/8251 ,  C12N15/8253 ,  C12N9/12 ,  C12N9/88 ,  C12P13/08

Abstract: A chimeric gene construct comprising a DNA sequence encoding an enzyme having aspartate kinase (AK) activity is provided, which is capable of expression in plant cells with subsequent increased production of threonine. Transgenic plants containing in their cells said chimeric gene overproduce threonine and transgenic plants containing in their cells said chimeric gene and a second chimeric gene comprising a DNA sequence coding for an enzyme having dihydrodipicolinate synthase (DHPS) activity, overproduce both threonine and lysine. The transgenic plants are resistant to lysine and threonine, to derivatives thereof and to selective inhibitors of the plant enzymes DHPS or AK, and thus these compounds may be used as selective herbicides in locus where the transgenic plants are cultivated.

Abstract translation: 提供了包含编码具有天冬氨酸激酶（AK）活性的酶的DNA序列的嵌合基因构建体，其能够在随后增加的苏氨酸的产生中在植物细胞中表达。 在其细胞中含有转基因植物，所述嵌合基因过量产生苏氨酸和在其细胞中含有所述嵌合基因的转基因植物，以及包含编码具有二氢吡啶二羧酸合酶（DHPS）活性的酶的DNA序列的第二嵌合基因，其过量产生苏氨酸和赖氨酸。 转基因植物对赖氨酸和苏氨酸，其衍生物和植物酶DHPS或AK的选择性抑制剂具有抗性，因此这些化合物可用作培养转基因植物的基因座中的选择性除草剂。

公开(公告)号：US4900676A

公开(公告)日：1990-02-13

申请号：US17870

申请日：1987-02-24

Applicant: Jonathan Gressel ,  Dvora Aviv ,  Avihai Perl

Inventor： Jonathan Gressel ,  Dvora Aviv ,  Avihai Perl

IPC: A01H1/06 ,  C12N5/14

CPC classification number: A01H1/06 ,  C12N5/14

Abstract: A method of introducing cytoplasmically inherited traits from a donor plant into a receptor plant of the Solanaceae family has been invented. A method is provided for producing Solanum tuberosum plants which have the inherited trait. Protoplasts from the donor and receptor plants are prepared. The donor protoplasts are treated under suitable conditions to prevent extensive nuclear divisions. The treated donor protoplasts and receptor protoplasts are then contacted under suitable conditions permitting the fusion of the protoplasts. Plantlets are regenerated from the fused protoplasts, and plantlets which contain the donor cytoplasmic traits are selected and rooted. Plantlets which contain recpetor cytoplasmic traits are discarded.

Abstract translation: 已经发明了将供体植物的细胞质遗传性状引入茄科的受体植物的方法。 提供了一种用于产生具有遗传性状的马铃薯（Solanum tuberosum）植物的方法。 来自供体和受体植物的原生质体被制备。 在合适的条件下处理供体原生质体以防止广泛的核分裂。 然后在允许原生质体融合的合适条件下接触处理的供体原生质体和受体原生质体。 从融合的原生质体再生植株，选择含有供体细胞质性的苗，并植根。 丢弃含有重要细胞质性状的植物。

公开(公告)号：US4795705A

公开(公告)日：1989-01-03

申请号：US704416

申请日：1985-02-22

Applicant: Jonathan Gressel ,  Dvora Aviv ,  Avihai Perl

Inventor： Jonathan Gressel ,  Dvora Aviv ,  Avihai Perl

IPC: A01H1/06 ,  C12N5/02 ,  C12N5/14 ,  C12N15/00 ,  A01H1/04 ,  C12N5/00

CPC classification number: A01H1/06 ,  C12N5/14

Abstract: A method of introducing cytoplasmically inherited traits from a donor plant into a receptor plant of the Solanaceae family has been invented. Protoplasts from the donor and receptor plants are prepared. The donor protoplasts are treated under suitable conditions to prevent extensive nuclear divisions. The treated donor protoplasts and receptor protoplasts are then contacted under suitable conditions permitting the fusion of the protoplasts. Plantlets are regenerated from the fused protoplasts, and plantlets which contain the donor cytoplasmic traits are selected and rooted. Plantlets which contain receptor cytoplasmic traits are discarded.

Abstract translation: 已经发明了将供体植物的细胞质遗传性状引入茄科的受体植物的方法。 来自供体和受体植物的原生质体被制备。 在合适的条件下处理供体原生质体以防止广泛的核分裂。 然后在允许原生质体融合的合适条件下接触处理的供体原生质体和受体原生质体。 从融合的原生质体再生植株，选择含有供体细胞质性的苗，并植根。 丢弃含有受体细胞质性状的植物。