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1.发明申请Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same 有权含有预生物异麦芽低聚糖的颗粒组合物及其制备和使用方法公开(公告)号：US20050031734A1
公开(公告)日：2005-02-10
申请号：US10798549
申请日：2004-03-10
申请人： Gang Duan , Feng Li , Jayarama Shetty , Julius Vadakoot , Sridevi Vadakoot
发明人： Gang Duan , Feng Li , Jayarama Shetty , Julius Vadakoot , Sridevi Vadakoot
IPC分类号： A21D2/18 , A23L1/09 , C07H3/06 , C12C5/00 , C12P19/04 , C12P19/14 , C12P19/18 , C12P19/22 , A21D2/00 , A21D8/02
摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.
摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使所述麦芽糖与转葡糖苷酶接触，其中所述步骤（a）和步骤（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。
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2.发明申请Grain Compositions Containing Pre-Biotic Isomal To-Oligosaccharides And Methods Of Making And Using Same 有权含有预生物异构异构寡糖的谷物组合物及其制备和使用方法公开(公告)号：US20120135108A1
公开(公告)日：2012-05-31
申请号：US13172744
申请日：2011-06-29
申请人： Gang Duan , Feng Li , Jayarama K. Shetty , Julius Vadakoot , Sridevi Vadakoot
发明人： Gang Duan , Feng Li , Jayarama K. Shetty , Julius Vadakoot , Sridevi Vadakoot
摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.
摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使所述麦芽糖与转葡糖苷酶接触，其中所述步骤（a）和步骤（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。
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3.发明授权Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same 有权含有预生物异麦芽低聚糖的颗粒组合物及其制备和使用方法公开(公告)号：US07638151B2
公开(公告)日：2009-12-29
申请号：US10798549
申请日：2004-03-10
申请人： Gang Duan , Feng Li , Jayarama K. Shetty , Julius Vadakoot , Sridevi Vadakoot, legal representative
发明人： Gang Duan , Feng Li , Jayarama K. Shetty , Julius Vadakoot
摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.
摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使所述麦芽糖与转葡糖苷酶接触，其中所述步骤（a）和步骤（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。
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4.发明授权Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same 有权含有预生物异麦芽低聚糖的颗粒组合物及其制备和使用方法公开(公告)号：US07993689B2
公开(公告)日：2011-08-09
申请号：US12616595
申请日：2009-11-11
申请人： Gang Duan , Fengi Li , Jayarama K. Shetty , Julius Vadakoot , Sridevi Vadakoot, legal representative
发明人： Gang Duan , Fengi Li , Jayarama K. Shetty , Julius Vadakoot
摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting the maltose with a transglucosidic enzyme, wherein the steps (a) and (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.
摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使麦芽糖与转葡糖苷酶接触，其中步骤（a）和（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。
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5.发明授权Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same 有权含有预生物异麦芽低聚糖的颗粒组合物及其制备和使用方法公开(公告)号：US08715755B2
公开(公告)日：2014-05-06
申请号：US13172744
申请日：2011-06-29
申请人： Gang Duan , Feng Li , Jayarama K. Shetty , Sridevi Vadakoot
发明人： Gang Duan , Feng Li , Jayarama K. Shetty , Julius Vadakoot
摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting the maltose with a transglucosidic enzyme, wherein the steps (a) and (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.
摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使麦芽糖与转葡糖苷酶接触，其中步骤（a）和（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。
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6.发明申请Production Of Maltotetraose Syrup Using A Pseudomonas Saccharophila Maltotetraohydrolase Variant 审中-公开使用糖酵母假单胞菌Maltotetraohydrolase变体生产麦芽四糖糖浆公开(公告)号：US20120301927A1
公开(公告)日：2012-11-29
申请号：US13263419
申请日：2010-04-08
申请人： Gang Duan , Sung Ho Lee , Ying Qian , Rafael F. Sala , Jayarama Shetty
发明人： Gang Duan , Sung Ho Lee , Ying Qian , Rafael F. Sala , Jayarama Shetty
IPC分类号： C12N9/28 , C12N15/63 , C12P19/14 , D06M16/00 , C12N1/19 , C12P7/14 , C12P19/16 , C12N15/56 , C12N1/21
CPC分类号： C12P19/14 , C12N9/2417 , C12P7/10 , C12P7/14 , C12P19/16 , D06L1/14 , D06M16/003 , Y02E50/16
摘要： Variants of a Pseudomonas saccharophila G4-forming amylase (PS4) advantageously can catalyze high temperature saccharification to produce maltotetraose syrup from a starch liquefact or granular starch, e.g., derived from cornstarch. The PS4 variants are useful in a process of saccharification of starch that advantageously produces significant amounts of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.
摘要翻译： 酿酒酵母G4形成淀粉酶（PS4）的变体有利地可以催化高温糖化以从淀粉液化或颗粒淀粉（例如衍生自玉米淀粉）产生麦芽四糖糖浆。 PS4变体可用于淀粉糖化过程，其有利地产生显着量的麦芽四糖，其可以在生产麦芽四糖糖浆的方法中在下游使用。 在一个实施方案中，提供了基于总糖含量可产生约40重量％至约60重量％麦芽四糖的耐热性PS4变体。
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7.发明申请Production Of Maltotetraose Syrup Using A Pseudomonas Saccharophila Maltotetraohydrolase Variant And A Debranching Enzyme 审中-公开使用糖酵母假单胞菌麦芽四氢水解酶变体和脱支酶生产麦芽四糖糖浆公开(公告)号：US20120135466A1
公开(公告)日：2012-05-31
申请号：US13263606
申请日：2010-04-08
申请人： Gang Duan , Ying Qian , Rafael F. Sala , Jayarama Shetty
发明人： Gang Duan , Ying Qian , Rafael F. Sala , Jayarama Shetty
IPC分类号： C12P19/00
CPC分类号： C12P19/14
摘要： The combination of a Pseudomonas saccharophila G4-forming amylase (PS4) variant and a pullulanase advantageously can catalyze at a high temperature saccharification to produce an increased amount of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant supplement with a pullulanase is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.
摘要翻译： 酿酒酵母G4形成淀粉酶（PS4）变体和支链淀粉酶的组合有利地可以在高温糖化下催化产生增加量的麦芽四糖，其可以在生产麦芽四糖糖浆的方法中用于下游。 在一个实施方案中，提供了具有支链淀粉酶的热稳定性PS4变体，其可以基于总糖含量产生约40重量％至约60重量％的麦芽四糖。
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公开(公告)号：US20060281157A1
公开(公告)日：2006-12-14
申请号：US11151919
申请日：2005-06-14
申请人： Gopal Chotani , Gang Duan , Craig Pilgrim , Jayarama Shetty
发明人： Gopal Chotani , Gang Duan , Craig Pilgrim , Jayarama Shetty
IPC分类号： C12P7/06
摘要： A dry solids staging fermentation process for producing an end-product, such as ethanol is disclosed said process including an initial fermentation step including combining a first fermentable substrate with one or more starch hydrolyzing enzymes and fermenting organisms in a fermentation vessel and a loading step which includes adding a second fermentable substrate to the fermentation vessel wherein the percent dry solids (% DS) of the fermentation broth increases over time.
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公开(公告)号：US20060154353A1
公开(公告)日：2006-07-13
申请号：US11314987
申请日：2005-12-21
CPC分类号： C12N9/58 , A23L13/424 , A23L33/18 , C12N9/48 , C12P7/06 , C12P7/10 , C12P19/02 , Y02E50/16 , Y02E50/17
摘要： The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.
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10.发明授权公开(公告)号：US09178386B2
公开(公告)日：2015-11-03
申请号：US13497512
申请日：2009-12-09
申请人： Gang Duan , Jingtao Wu , Xiaoyan Sun , Dong Yang
发明人： Gang Duan , Jingtao Wu , Xiaoyan Sun , Dong Yang
CPC分类号： H02J13/0006 , G01R19/2513 , Y02E60/728 , Y02E60/74 , Y04S10/265 , Y04S10/30
摘要： A state-matrix-independent dynamic process estimation method in real-time for weakly observable measurement nodes without Phasor Measurement Unit(PMU) is only dependent on real-time measurement dynamic data of measurement nodes with PMU and measurement data of Supervisory Control And Data Acquisition (SCADA) system in electric power system or state estimation data. According to the SCADA measurement data or state estimation data at some continuous moments, the method utilizes recursive least squares solution to find a linear combination relationship between variation of measurement parameter to be estimated of nodes without PMU and variation of corresponding measurement parameter of nodes with PMU. Using the linear combination of relationship, the dynamic process of measurement nodes without PMU is estimated in real-time. The method provides high estimation precision and meets error requirements of engineering application.
摘要翻译： 弱相关测量单元（PMU）的弱观测测量节点的状态矩无关动态过程估计方法仅取决于PMU测量节点与监控和数据采集测量数据的实时测量动态数据 （SCADA）系统或状态估计数据。 根据SCADA测量数据或某些连续时刻的状态估计数据，该方法利用递归最小二乘解求解PMU节点测量参数变化与PMU节点对应测量参数变化之间的线性组合关系 。 使用线性关系的组合，实时估计无PMU的测量节点的动态过程。 该方法提供高估计精度，满足工程应用的误差要求。
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