公开(公告)号：US20050176095A1

公开(公告)日：2005-08-11

申请号：US10504988

申请日：2002-08-20

申请人： Genryou Umitsuki ,  Osamu Hatamoto ,  Seiichi Hara ,  Tsutomu Masuda ,  Motoaki Sano ,  Masayuki Machida

发明人： Genryou Umitsuki ,  Osamu Hatamoto ,  Seiichi Hara ,  Tsutomu Masuda ,  Motoaki Sano ,  Masayuki Machida

IPC分类号： C12N15/09 ,  C07K14/38 ,  C12N1/14 ,  C12N1/15 ,  C12N9/50 ,  C12N9/62 ,  C12N15/31 ,  C12P21/00 ,  C12R1/69 ,  C12P21/06 ,  C07K14/37 ,  C12N1/16 ,  C12N15/74

CPC分类号： C07K14/38

摘要： This invention provides a transcription factor that has functions of regulating the expression of a sulfur-assimilatory gene of koji mold during culture, and a gene encoding the same. This invention also relates to a protein comprising the amino acid sequence as shown in SEQ ID NO: 3, and a gene encoding the amino acid sequence as shown in SEQ ID NO: 3 or comprising the nucleotide sequence as shown in SEQ ID NO: 2.

摘要翻译： 本发明提供了具有调节培养期间曲霉的硫同化基因的表达的功能的转录因子和编码其的基因。 本发明还涉及包含SEQ ID NO：3所示的氨基酸序列的蛋白质和编码SEQ ID NO：3所示的氨基酸序列的基因，或包含SEQ ID NO：2所示的核苷酸序列 。

公开(公告)号：US20090130711A1

公开(公告)日：2009-05-21

申请号：US11990926

申请日：2006-09-19

申请人： Osamu Hatamoto ,  Genryou Umitsuki ,  Masayuki Machida ,  Motoaki Sano ,  Akimitsu Tanaka ,  Chitoshi Oka ,  Hiroshi Maeda ,  Hitoshi Tainaka ,  Touru Ito ,  Tomomi Uchikawa ,  Tsutomu Masuda ,  Kenichiro Matsushima

发明人： Osamu Hatamoto ,  Genryou Umitsuki ,  Masayuki Machida ,  Motoaki Sano ,  Akimitsu Tanaka ,  Chitoshi Oka ,  Hiroshi Maeda ,  Hitoshi Tainaka ,  Touru Ito ,  Tomomi Uchikawa ,  Tsutomu Masuda ,  Kenichiro Matsushima

IPC分类号： C12P21/04 ,  C12N15/00 ,  C12N15/11 ,  C07K14/00 ,  C12N1/19

CPC分类号： C12N15/80 ,  A23L11/09 ,  A23L27/24 ,  A23L27/50 ,  C07K14/38 ,  C12N9/62 ,  C12P21/02

摘要： To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.

摘要翻译： 改善在固体或液体培养基中生产食品（例如调味品），药物（例如消化剂），用于洗涤剂等的蛋白酶中的Koji模具蛋白酶的活性。 公开了具有增加Koji模型蛋白酶的分泌能力的重组载体，用载体转化并具有增加的蛋白酶基因表达或其增加分泌的Koji模型，生产方法 的蛋白酶通过使用转化的Koji模具等。

公开(公告)号：US06951750B2

公开(公告)日：2005-10-04

申请号：US10325939

申请日：2002-12-23

申请人： Kotaro Ito ,  Takeharu Nakahara ,  Yasuji Koyama ,  Toshifumi Matsuda ,  Tadashi Takahashi ,  Kenichiro Matsushima ,  Genryou Umitsuki ,  Tsutomu Masuda

发明人： Kotaro Ito ,  Takeharu Nakahara ,  Yasuji Koyama ,  Toshifumi Matsuda ,  Tadashi Takahashi ,  Kenichiro Matsushima ,  Genryou Umitsuki ,  Tsutomu Masuda

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/48 ,  C12N9/62 ,  C12N15/57

CPC分类号： C12Y304/13009 ,  C12N9/48

摘要： Provided are a protein comprising the amino acid sequence as shown in SEQ ID NO: 2 and having prolidase activities, a prolidase gene coding for the protein, a prolidase gene comprising the nucleotide sequence as shown in SEQ ID NO: 1, recombinant DNA having the gene inserted into vector DNA, and a transformant or transductant comprising the recombinant DNA, and a method for producing prolidase using the transformant or transductant.The present invention can improve the prolidase through protein engineering. The present invention can be also used for improving microorganisms used in the production of enzymes for food processing and fermented foods.

摘要翻译： 提供了包含SEQ ID NO：2所示的氨基酸序列并具有唾液酸酶活性的蛋白质，编码该蛋白质的脯氨酶基因，包含SEQ ID NO：1所示的核苷酸序列的唾液酸酶基因，具有SEQ ID NO： 插入到载体DNA中的基因，以及包含重组DNA的转化体或转导体，以及使用转化体或转导体产生唾液酸酶的方法。 本发明可以通过蛋白质工程改善脯氨酸酶。 本发明还可以用于改善用于食品加工和发酵食品酶生产的微生物。

公开(公告)号：US6127161A

公开(公告)日：2000-10-03

申请号：US330095

申请日：1999-06-11

申请人： Genryou Umitsuki ,  Keietsu Abe

发明人： Genryou Umitsuki ,  Keietsu Abe

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/48 ,  C12N15/00 ,  C12N15/57 ,  C12Q1/68 ,  C12R1/66 ,  C07H21/04 ,  C12N1/20

CPC分类号： C12N9/48

摘要： The present invention relates to an Aspergillus sojae leucine aminopeptidase gene or variants thereof, a recombinant DNA including the gene or the variants, and a process for producing leucine aminopeptidase using the recombinant DNA.

摘要翻译： 本发明涉及一种Aspergillus sojae亮氨酸氨基肽酶基因或其变体，包含该基因或变体的重组DNA，以及使用重组DNA产生亮氨酸氨基肽酶的方法。

公开(公告)号：US06706492B2

公开(公告)日：2004-03-16

申请号：US09801734

申请日：2001-03-09

申请人： Genryou Umitsuki ,  Hiroe Sato ,  Misao Sugishita ,  Yaichi Fukushima ,  Yasuji Koyama

发明人： Genryou Umitsuki ,  Hiroe Sato ,  Misao Sugishita ,  Yaichi Fukushima ,  Yasuji Koyama

IPC分类号： C12N115

CPC分类号： C12N1/14 ,  A23J3/346 ,  A23L27/50 ,  C12N9/62

摘要： The present invention provides a koji mold having increased protease activity and peptidase activity relative to a parent strain, a method of breeding the koji mold, and a method of manufacturing a flavor enhancer using the koji mold. More specifically, the present invention provides (1) a koji mold having increased protease activity and peptidase activity relative to a parent strain obtained by transformation using a protease gene and a peptidase gene, (2) a method of breeding the above koji mold which comprises transforming a parent strain of koji mold using a protease gene and a peptidase gene, and then selecting a transformant having higher protease activity and peptidase activity relative to a parent strain, and (3) a method of manufacturing a flavor enhancer which comprises allowing a culture product of the above koji mold to act on a protein.

摘要翻译： 本发明提供了相对于亲本菌株具有增加的蛋白酶活性和肽酶活性的曲霉菌，使用该曲霉菌制造风味增强剂的方法。 更具体地，本发明提供了（1）相对于通过使用蛋白酶基因和肽酶基因转化而获得的亲本菌株，具有增加的蛋白酶活性和肽酶活性的曲霉，（2）育种上述曲霉菌的方法，其包含 使用蛋白酶基因和肽酶基因转化真菌的母本菌株，然后选择相对于亲本菌株具有较高蛋白酶活性和肽酶活性的转化体，和（3）制备风味增强剂的方法，其包括使培养物 上述曲霉菌的产物作用于蛋白质。

公开(公告)号：US06541236B2

公开(公告)日：2003-04-01

申请号：US09946678

申请日：2001-09-06

申请人： Kotaro Ito ,  Genryou Umitsuki ,  Yasuji Koyama

发明人： Kotaro Ito ,  Genryou Umitsuki ,  Yasuji Koyama

IPC分类号： C12N978

CPC分类号： C12N9/80 ,  C12Y305/01002

摘要： There are disclosed a protein having an amino acid sequence represented by amino acid numbers 1 to 684 or 49 to 684 shown in SEQ ID NO:2, or a protein having a glutaminase activity in which one or more amino acids is/are deleted from, substituted by, inserted to or added to the amino acid sequence of the above protein; a gene containing DNA encoding the above protein or a gene encoding a protein which hybridizes with the DNA of the above gene under a stringent condition and has a glutaminase activity; a recombinant DNA containing the above gene; a transformant or a transductant containing the above recombinant DNA; and a process for producing glutaminase which comprises culturing the above transformant or the above transductant and collecting glutaminase from a culture medium.