公开(公告)号：US6096519A

公开(公告)日：2000-08-01

申请号：US969644

申请日：1997-11-13

申请人： Givlio Ratti ,  Maurizio Comanducci ,  Mario F. Tecce ,  Marzia M. Giuliani

发明人： Givlio Ratti ,  Maurizio Comanducci ,  Mario F. Tecce ,  Marzia M. Giuliani

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/295 ,  C07K16/12 ,  C12N15/30 ,  C12N15/74 ,  C12P21/02 ,  C07K1/00 ,  G01N33/543 ,  G01N33/554

CPC分类号： C07K14/295 ,  C07K16/125 ,  C12N15/74 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/75

摘要： A plasmid isolated from Clamydia trachomatis is described, which comprises 8 genes encoding proteins useful in the formulation of vaccines or diagnostic test for determining the bacterium or specific antibodies generated during C. trachomatis infections; in particular the recombinant fusion MS2-pgp3D protein is described comprising polypeptidic sequences encoded by pCT and immunogenic in the course of infections in man. A method for preparing said protein in E. coli further described.

摘要翻译： 描述了从沙眼衣原体分离的质粒，其包含编码用于疫苗制剂的蛋白质的8种基因或用于确定沙眼衣原体感染期间产生的细菌或特异性抗体的诊断试验; 特别地描述了重组融合物MS2-pgp3D蛋白质，其包括由pCT编码的多肽序列和在人类感染过程中具有免疫原性。 进一步描述在大肠杆菌中制备所述蛋白质的方法。

公开(公告)号：US6110705A

公开(公告)日：2000-08-29

申请号：US444189

申请日：1995-05-18

申请人： Givlio Ratti ,  Maurizio Comanducci ,  Mario F. Tecce ,  Marzia M. Giuliani

发明人： Givlio Ratti ,  Maurizio Comanducci ,  Mario F. Tecce ,  Marzia M. Giuliani

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/295 ,  C07K16/12 ,  C12N15/30 ,  C12N15/74 ,  C12P21/06 ,  C12P21/04

CPC分类号： C07K14/295 ,  C07K16/125 ,  C12N15/74 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/75

摘要： A plasmid isolated from Chlamydia trachomatis is described, which comprises 8 genes encoding proteins useful in the formation of vaccines or diagnostic test for determining the bacterium or specific antibodies generated during C. trachomatis infections. In particular, the recombinant fusion protein MS2-pgp3D is described, which comprises polypeptide sequences encoded by pCT and is immunogenic in the course of infections in man. A method for preparing the recombinant fusion protein MS2-pgp3D in E. coli is also described.

摘要翻译： 描述了从沙眼衣原体分离的质粒，其包含编码用于疫苗形成的蛋白质的8种基因或用于确定在沙眼衣原体感染期间产生的细菌或特异性抗体的诊断试验。 特别地，描述了重组融合蛋白MS2-pgp3D，其包含由pCT编码的多肽序列，并且在人的感染过程中是免疫原性的。 还描述了在大肠杆菌中制备重组融合蛋白MS2-pgp3D的方法。

公开(公告)号：US06248563B1

公开(公告)日：2001-06-19

申请号：US08468544

申请日：1995-06-06

申请人： Giulio Ratti ,  Maurizio Comanducci ,  Mario F. Tecce ,  Marzia M. Giuliani

发明人： Giulio Ratti ,  Maurizio Comanducci ,  Mario F. Tecce ,  Marzia M. Giuliani

IPC分类号： C12P2106

CPC分类号： C07K14/295 ,  A61K38/00 ,  A61K39/00 ,  C07K16/125 ,  C07K2319/00 ,  C08G73/1042 ,  C08G73/1053 ,  C08G73/1071 ,  C12N15/74

摘要： A plasmid isolated from Clamydia trachomatis is described, which comprises 8 genes encoding proteins useful in the formulation of vaccines or diagnostic test for determining the bacterium or specific antibodies generated during C. trachomatis infections; in particular the recombinant fusion MS2-pgp3D protein is described comprising polypeptidic sequences encoded by pCT and immunogenic in the course of infections in man. A method for preparing said protein in E. coli further described.

摘要翻译： 描述了从沙眼衣原体分离的质粒，其包含编码用于疫苗制剂的蛋白质的8种基因或用于确定沙眼衣原体感染期间产生的细菌或特异性抗体的诊断试验; 特别地描述了重组融合物MS2-pgp3D蛋白质，其包括由pCT编码的多肽序列和在人类感染过程中具有免疫原性。 进一步描述在大肠杆菌中制备所述蛋白质的方法。

公开(公告)号：US5130415A

公开(公告)日：1992-07-14

申请号：US384411

申请日：1989-07-24

申请人： Mario F. Tecce ,  Marzia M. Giuliani ,  Stefano Ricci ,  Giulio Ratti ,  Benedetto Terrana

发明人： Mario F. Tecce ,  Marzia M. Giuliani ,  Stefano Ricci ,  Giulio Ratti ,  Benedetto Terrana

IPC分类号： G01N33/574 ,  C07K14/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/30 ,  C07K19/00 ,  C12N1/21 ,  C12N9/12 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  G01N33/577

CPC分类号： C12N9/1252 ,  C07K14/4715 ,  C07K16/30 ,  C07K16/303 ,  C07K2319/00 ,  C07K2319/30

摘要： A synthetic peptide endowed with immunological activity is disclosed, which is capable of inducing in mammals the formation of anti-peptide antibodies capable of recognizing human alpha-fetoprotein and of reacting with it, and incapable of recognizing albumin or its degradation products, with said peptide being essentially constituted by the aminoacidic sequence of formula (I), corresponding to 38-119 region of human alpha-fetoprotein.Furthermore, the methods and means for preparing said peptide by means of the recombinant DNA techniques are reported.The synthetic peptide (I), like the polyclonal and monoclonal antipeptide antibodies are particularly suitable for the early diagnosis of hepatocellular carcinoma and of teratocarcinoma.

公开(公告)号：US5206164A

公开(公告)日：1993-04-27

申请号：US835278

申请日：1992-02-13

申请人： Mario F. Tecce ,  Marzia M. Giuliani ,  Sterfano Ricci ,  Giulio Rttil ,  Benedetto Terrana

发明人： Mario F. Tecce ,  Marzia M. Giuliani ,  Sterfano Ricci ,  Giulio Rttil ,  Benedetto Terrana

IPC分类号： C07K14/47 ,  C07K16/30 ,  C12N1/21 ,  C12N9/12 ,  C12N15/12

CPC分类号： C12N9/1252 ,  C07K14/4715 ,  C07K16/30 ,  C07K16/303 ,  C07K2319/31 ,  C07K2319/40

摘要： A synthetic peptide endowed with immunological activity is disclosed, which is capable of inducing in mammals the formation of anti-peptide antibodies capable of recognizing human alpha-fetoprotein and of reacting with it, and incapable of recognizing albumin or its degradation products, with said peptide being essentially constituted by the aminoacidic sequence of formula (I), corresponding to 38-119 region of human alpha-fetoprotein.Furthermore, the methods and means for preparing said peptide by means of the recombinant DNA techniques are reported.The synthetic peptide (I), like the polyclonal and monoclonal antipeptide antibodies are particularly suitable for the early diagnosis of hepatocellular carcinoma and of teratocarcinoma.

摘要翻译： 公开了具有免疫学活性的合成肽，其能够在哺乳动物中诱导能够识别人甲胎蛋白并与其反应并且不能识别白蛋白或其降解产物的抗肽抗体与所述肽 基本上由式（I）的氨基酸序列构成，对应于人甲胎蛋白的38-119区域。 此外，报道了通过重组DNA技术制备所述肽的方法和方法。 合成肽（I），如多克隆和单克隆抗肽抗体特别适用于肝细胞癌和畸胎癌的早期诊断。