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公开(公告)号：US5508264A
公开(公告)日：1996-04-16
申请号：US349867
申请日：1994-12-06
IPC分类号： C12N15/09 , A01N37/20 , A01N63/02 , C07K7/06 , C07K7/08 , C07K14/325 , C12N1/21 , C12N15/32 , C12P21/02 , C12R1/19 , C12R1/39 , A61K38/16
CPC分类号： C07K14/325 , A01N63/02 , C07K2319/00
摘要： Disclosed are compositions and processes for controlling lepidopteran pests. These compositions comprise synergistic combinations of a CryIF chimeric and CryIA(c) chimeric Bacillus thuringiensis .delta.-endotoxin. These compositions have been found to exhibit excellent activity against lepidopteran pests.
摘要翻译： 披露了控制鳞翅目害虫的组合物和方法。 这些组合物包含CryIF嵌合和CryIA（c）嵌合苏云金芽孢杆菌δ-内毒素的协同组合。 已经发现这些组合物对鳞翅目害虫表现出优异的活性。
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公开(公告)号：US5827514A
公开(公告)日：1998-10-27
申请号：US598305
申请日：1996-02-08
IPC分类号： C12N15/09 , A01N37/20 , A01N63/02 , C07K7/06 , C07K7/08 , C07K14/325 , C12N1/21 , C12N15/32 , C12P21/02 , C12R1/19 , C12R1/39 , A01N63/00 , C12N5/14
CPC分类号： C07K14/325 , A01N63/02 , C07K2319/00
摘要： Disclosed are compositions and processes for controlling lepidopteran pests. These compositions comprise synergistic combinations of a CryIF chimeric and CryIA(c) chimeric Bacillus thuringiensis .delta.-endotoxin. These compositions have been found to exhibit excellent activity against lepidopteran pests.
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3.发明授权Bacillus thuringiensis isolates, toxins, and genes for controlling certain coleopteran pests 失效苏云金芽孢杆菌分离株，毒素和控制某些鞘翅目害虫的基因
公开(公告)号：US5973231A
公开(公告)日：1999-10-26
申请号：US76193
申请日：1998-05-12
CPC分类号： C07K14/325
摘要： The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention concerns novel genes and pesticidal toxins referred to as 86A1(b) and 52A1(b). In preferred embodiments, the subject toxins are used for controlling flea beetles of the genus Phyllotreta. Using the genes described herein, the transformation of plants can be accomplished using techniques known to those skilled in the art. In addition, the subject invention provides toxin genes optimized for expression in plants.
摘要翻译： 本发明涉及可用于防治害虫，特别是植物害虫的材料和方法。 更具体地，本发明涉及被称为86A1（b）和52A1（b）的新基因和杀虫毒素。 在优选的实施方案中，本发明的毒素用于控制Phyllotreta属的跳蚤甲虫。 使用本文所述的基因，可以使用本领域技术人员已知的技术来实现植物的转化。 此外，本发明提供了优化用于在植物中表达的毒素基因。
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4.发明授权公开(公告)号：US06710027B2
公开(公告)日：2004-03-23
申请号：US09991582
申请日：2001-11-16
IPC分类号： A01N3718
CPC分类号： C07K14/325
摘要： The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention concerns novel genes and pesticidal toxins referred to as 86A1(b) and 52A1(b). In preferred embodiments, the subject toxins are used for controlling flea beetles of the genus Phyllotreta. Using the genes described herein, the transformation of plants can be accomplished using techniques known to those skilled in the art. In addition, the subject invention provides toxin genes optimized for expression in plants.
摘要翻译： 本发明涉及可用于防治害虫，特别是植物害虫的材料和方法。 更具体地，本发明涉及被称为86A1（b）和52A1（b）的新基因和杀虫毒素。 在优选的实施方案中，本发明的毒素用于控制Phyllotreta属的跳蚤甲虫。 使用本文所述的基因，可以使用本领域技术人员已知的技术来实现植物的转化。 此外，本发明提供了优化用于在植物中表达的毒素基因。
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5.发明授权公开(公告)号：US06344553B1
公开(公告)日：2002-02-05
申请号：US09307925
申请日：1999-05-10
IPC分类号： C12N121
CPC分类号： C07K14/325
摘要： The subject invention concerns materials and methods useful in the control of pests and, particularly, the plant pests. More specifically, the subject invention concerns novel genes and pesticidal toxins referred to as 86A1(b) and 52A1(b). In preferred embodiments, the subject toxins are used for controlling flea beetles of the genus Phyllotreta. Using the genes described herein, the transformation of plants can be accomplished using techniques known to those skilled in the art. In addition, the subject invention provides toxin genes optimized for expression in plants.
摘要翻译： 本发明涉及可用于防治害虫，特别是植物害虫的材料和方法。 更具体地，本发明涉及被称为86A1（b）和52A1（b）的新基因和杀虫毒素。 在优选的实施方案中，本发明的毒素用于控制Phyllotreta属的跳蚤甲虫。 使用本文所述的基因，可以使用本领域技术人员已知的技术来实现植物的转化。 此外，本发明提供了优化用于在植物中表达的毒素基因。
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公开(公告)号：US5932209A
公开(公告)日：1999-08-03
申请号：US732495
申请日：1997-03-06
IPC分类号： A01M1/20 , A01N63/02 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/91 , A01N63/00 , A01N37/18
CPC分类号： C07K14/325 , C07K2319/00 , Y10S424/832 , Y10S530/825
摘要： An improved Bacillus thuringiensis (B.t.) delta-endotoxin is created by the modification of the gene encoding the toxin. The toxicity of a B.t. toxin was improved by replacing the native protoxin segment with an alternate protoxin segment by constructing a chimeric toxin gene.
摘要翻译： PCT No.PCT / US95 / 05090 Sec。 371日期1997年3月6日 102（e）日期1997年3月6日PCT提交1995年5月5日PCT公布。 WO95 / 30752 PCT出版物 日期1995年11月16日改良的苏云金芽孢杆菌（B.t.）δ-内毒素是通过修饰编码毒素的基因产生的。 B.t.的毒性 通过构建嵌合毒素基因，用替代的原毒素片段代替天然原毒素片段来改善毒素。
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公开(公告)号：US5290914A
公开(公告)日：1994-03-01
申请号：US187167
申请日：1988-04-28
IPC分类号： C12N9/00 , A01N63/00 , C07K14/00 , C07K14/195 , C07K14/325 , C07K14/34 , C07K14/41 , C07K14/415 , C07K19/00 , C12N1/21 , C12N15/62 , C12P21/02 , C12R1/38 , C07K13/00 , C12N15/00 , C12P21/06
CPC分类号： C12N15/62 , C07K14/325 , C07K14/34 , C07K2319/035 , C07K2319/55
摘要： The invention concerns novel hybrid pesticidal toxins. These toxins are expressed as the fusion protein of a chimeric gene. Specifically exemplified is a novel B.t. hybrid toxin. These novel toxins have increased toxicity against target pests. The invention also concerns a process for preparing a hybrid virus having an altered insect host range.
摘要翻译： 本发明涉及新型杂种杀虫毒素。 这些毒素被表达为嵌合基因的融合蛋白。 具体例子是一个小说B.t. 杂交毒素。 这些新型毒素对目标害虫的毒性增加。 本发明还涉及制备具有改变的昆虫宿主范围的杂种病毒的方法。
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公开(公告)号：US6051556A
公开(公告)日：2000-04-18
申请号：US438465
申请日：1995-05-10
IPC分类号： C07K14/325 , C07K14/34 , C12N15/62 , A61K38/00 , C07K1/00
CPC分类号： C12N15/62 , C07K14/325 , C07K14/34 , C07K2319/00 , C07K2319/035 , C07K2319/55
摘要： The invention concerns novel hybrid pesticidal toxins. These toxins are expressed as the fusion protein of a chimeric gene. Specifically exemplified is a novel B.t. hybrid toxin. These novel toxins have increased toxicity against target pests. The invention also concerns a process for preparing a hybrid virus having an altered insect host range.
摘要翻译： 本发明涉及新型杂种杀虫毒素。 这些毒素被表达为嵌合基因的融合蛋白。 具体例子是一个小说B.t. 杂交毒素。 这些新型毒素对目标害虫的毒性增加。 本发明还涉及制备具有改变的昆虫宿主范围的杂种病毒的方法。
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公开(公告)号：US5840554A
公开(公告)日：1998-11-24
申请号：US639923
申请日：1996-04-24
申请人： Mark Thompson , George E. Schwab
发明人： Mark Thompson , George E. Schwab
IPC分类号： C12N15/09 , A01N63/00 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/39 , C12N15/11 , C12N15/78
CPC分类号： A01N63/00 , C07K14/325 , C07K2317/24 , C07K2319/00
摘要： Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryI(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomonads. The invention also concerns novel genes and plasmids.
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公开(公告)号：US5527883A
公开(公告)日：1996-06-18
申请号：US239476
申请日：1994-05-06
申请人： Mark Thompson , George E. Schwab
发明人： Mark Thompson , George E. Schwab
IPC分类号： C12N15/09 , A01N63/00 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/39 , C12N15/62 , C12N15/78
CPC分类号： A01N63/00 , C07K14/325 , C07K2317/24 , C07K2319/00
摘要： Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryI(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomonads. The invention also concerns novel genes and plasmids.
摘要翻译： 通过修饰编码苏云金芽孢杆菌内毒素的基因可以改善拟南芥中苏云金芽孢杆菌内毒素的表达。 通过用编码不同原毒素的片段替代编码天然原毒素的苏云金芽孢杆菌基因片段来产生嵌合基因。 本文例示的是cryIF / cryI（b）嵌合体，其中天然cryIF原毒素区段已被cryIA（b）原毒素区段取代，以产生在假单胞菌中cryIF毒素的改进的表达。 本发明还涉及新基因和质粒。
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