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公开(公告)号：US5508264A
公开(公告)日：1996-04-16
申请号：US349867
申请日：1994-12-06
IPC分类号： C12N15/09 , A01N37/20 , A01N63/02 , C07K7/06 , C07K7/08 , C07K14/325 , C12N1/21 , C12N15/32 , C12P21/02 , C12R1/19 , C12R1/39 , A61K38/16
CPC分类号： C07K14/325 , A01N63/02 , C07K2319/00
摘要： Disclosed are compositions and processes for controlling lepidopteran pests. These compositions comprise synergistic combinations of a CryIF chimeric and CryIA(c) chimeric Bacillus thuringiensis .delta.-endotoxin. These compositions have been found to exhibit excellent activity against lepidopteran pests.
摘要翻译： 披露了控制鳞翅目害虫的组合物和方法。 这些组合物包含CryIF嵌合和CryIA（c）嵌合苏云金芽孢杆菌δ-内毒素的协同组合。 已经发现这些组合物对鳞翅目害虫表现出优异的活性。
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公开(公告)号：US5827514A
公开(公告)日：1998-10-27
申请号：US598305
申请日：1996-02-08
IPC分类号： C12N15/09 , A01N37/20 , A01N63/02 , C07K7/06 , C07K7/08 , C07K14/325 , C12N1/21 , C12N15/32 , C12P21/02 , C12R1/19 , C12R1/39 , A01N63/00 , C12N5/14
CPC分类号： C07K14/325 , A01N63/02 , C07K2319/00
摘要： Disclosed are compositions and processes for controlling lepidopteran pests. These compositions comprise synergistic combinations of a CryIF chimeric and CryIA(c) chimeric Bacillus thuringiensis .delta.-endotoxin. These compositions have been found to exhibit excellent activity against lepidopteran pests.
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公开(公告)号：US5186934A
公开(公告)日：1993-02-16
申请号：US715184
申请日：1991-06-12
IPC分类号： C07K14/325 , C12N1/21 , C12N15/32 , C12P1/04
CPC分类号： C07K14/325 , C12P1/04
摘要： A novel B.t. gene encoding a toxin which is toxic to coleopteran insects has been cloned from a coleopteran-active B. thurningiensis microbe. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control coleopteran insects in various environments.
摘要翻译： 小说B.t. 编码对鞘翅目昆虫有毒的毒素的基因已经从鞘翅目昆虫活性B.thurningiensis微生物中克隆出来。 编码B.t.的DNA 毒素可用于转化各种原核和真核微生物，以表达B.t. 毒素。 这些重组微生物可用于控制各种环境中的鞘翅目昆虫。
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公开(公告)号：US5286485A
公开(公告)日：1994-02-15
申请号：US758020
申请日：1991-09-12
申请人： Kendrick A. Uyeda , Luis Foncerrada , Jewel M. Payne , Kenneth E. Narva , George E. Schwab , Gregory A. Bradfisch , August J. Sick
发明人： Kendrick A. Uyeda , Luis Foncerrada , Jewel M. Payne , Kenneth E. Narva , George E. Schwab , Gregory A. Bradfisch , August J. Sick
IPC分类号： A01N63/00 , C07K14/325 , C12N15/82 , C12P1/04 , C12N1/20
CPC分类号： A01N63/00 , C07K14/325 , C12N15/8286 , C12P1/04 , C12R1/075
摘要： The subject disclosure concerns Bacillus thuringiensis strains which can be used to control lepidopteran pests. The strains were previously known to control coleopteran pests. The discovery of lepidopteran activity was totally unexpected.These B.t. strains can be formulated using standard lepidopteran formulation procedures. Means of administration are also standard.The genes encoding lepidopteran-active toxins can be isolated from the B.t. isolates and used to transform other microbes for use to control lepidopteran pests.
摘要翻译： 本发明涉及可用于控制鳞翅目害虫的苏云金芽孢杆菌菌株。 以前已知菌株可以控制鞘翅目害虫。 发现鳞翅目的活动是完全意想不到的。 这些B.t. 菌株可以使用标准鳞翅目配方程序进行配制。 行政手段也是标准。 编码鳞翅目昆虫活性毒素的基因可以从B.t. 分离并用于转化其他微生物用于控制鳞翅目害虫。
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5.发明授权
公开(公告)号：US5430137A
公开(公告)日：1995-07-04
申请号：US968781
申请日：1992-10-30
申请人： Frank H. Gaertner , August J. Sick , Mark Thompson , H. Ernest Schnepf , George E. Schwab , Kenneth E. Narva
发明人： Frank H. Gaertner , August J. Sick , Mark Thompson , H. Ernest Schnepf , George E. Schwab , Kenneth E. Narva
CPC分类号： C12Q1/689
摘要： This invention concerns novel gene probes which can be used to identify DNA from Bacillus thuringiensis microbes which encode protein endotoxins. The invention probes greatly facilitate the search for useful microbes hosting genes which encode toxins.
摘要翻译： 本发明涉及可用于鉴定编码蛋白质内毒素的苏云金芽孢杆菌微生物DNA的新型基因探针。 本发明探针极大地促进了寻找编码毒素的基因的有用微生物。
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公开(公告)号：US5932209A
公开(公告)日：1999-08-03
申请号：US732495
申请日：1997-03-06
IPC分类号： A01M1/20 , A01N63/02 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/91 , A01N63/00 , A01N37/18
CPC分类号： C07K14/325 , C07K2319/00 , Y10S424/832 , Y10S530/825
摘要： An improved Bacillus thuringiensis (B.t.) delta-endotoxin is created by the modification of the gene encoding the toxin. The toxicity of a B.t. toxin was improved by replacing the native protoxin segment with an alternate protoxin segment by constructing a chimeric toxin gene.
摘要翻译： PCT No.PCT / US95 / 05090 Sec。 371日期1997年3月6日 102（e）日期1997年3月6日PCT提交1995年5月5日PCT公布。 WO95 / 30752 PCT出版物 日期1995年11月16日改良的苏云金芽孢杆菌（B.t.）δ-内毒素是通过修饰编码毒素的基因产生的。 B.t.的毒性 通过构建嵌合毒素基因，用替代的原毒素片段代替天然原毒素片段来改善毒素。
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公开(公告)号：US5290914A
公开(公告)日：1994-03-01
申请号：US187167
申请日：1988-04-28
IPC分类号： C12N9/00 , A01N63/00 , C07K14/00 , C07K14/195 , C07K14/325 , C07K14/34 , C07K14/41 , C07K14/415 , C07K19/00 , C12N1/21 , C12N15/62 , C12P21/02 , C12R1/38 , C07K13/00 , C12N15/00 , C12P21/06
CPC分类号： C12N15/62 , C07K14/325 , C07K14/34 , C07K2319/035 , C07K2319/55
摘要： The invention concerns novel hybrid pesticidal toxins. These toxins are expressed as the fusion protein of a chimeric gene. Specifically exemplified is a novel B.t. hybrid toxin. These novel toxins have increased toxicity against target pests. The invention also concerns a process for preparing a hybrid virus having an altered insect host range.
摘要翻译： 本发明涉及新型杂种杀虫毒素。 这些毒素被表达为嵌合基因的融合蛋白。 具体例子是一个小说B.t. 杂交毒素。 这些新型毒素对目标害虫的毒性增加。 本发明还涉及制备具有改变的昆虫宿主范围的杂种病毒的方法。
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公开(公告)号：US6051556A
公开(公告)日：2000-04-18
申请号：US438465
申请日：1995-05-10
IPC分类号： C07K14/325 , C07K14/34 , C12N15/62 , A61K38/00 , C07K1/00
CPC分类号： C12N15/62 , C07K14/325 , C07K14/34 , C07K2319/00 , C07K2319/035 , C07K2319/55
摘要： The invention concerns novel hybrid pesticidal toxins. These toxins are expressed as the fusion protein of a chimeric gene. Specifically exemplified is a novel B.t. hybrid toxin. These novel toxins have increased toxicity against target pests. The invention also concerns a process for preparing a hybrid virus having an altered insect host range.
摘要翻译： 本发明涉及新型杂种杀虫毒素。 这些毒素被表达为嵌合基因的融合蛋白。 具体例子是一个小说B.t. 杂交毒素。 这些新型毒素对目标害虫的毒性增加。 本发明还涉及制备具有改变的昆虫宿主范围的杂种病毒的方法。
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公开(公告)号：US5840554A
公开(公告)日：1998-11-24
申请号：US639923
申请日：1996-04-24
申请人： Mark Thompson , George E. Schwab
发明人： Mark Thompson , George E. Schwab
IPC分类号： C12N15/09 , A01N63/00 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/39 , C12N15/11 , C12N15/78
CPC分类号： A01N63/00 , C07K14/325 , C07K2317/24 , C07K2319/00
摘要： Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryI(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomonads. The invention also concerns novel genes and plasmids.
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公开(公告)号：US5527883A
公开(公告)日：1996-06-18
申请号：US239476
申请日：1994-05-06
申请人： Mark Thompson , George E. Schwab
发明人： Mark Thompson , George E. Schwab
IPC分类号： C12N15/09 , A01N63/00 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/39 , C12N15/62 , C12N15/78
CPC分类号： A01N63/00 , C07K14/325 , C07K2317/24 , C07K2319/00
摘要： Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryI(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomonads. The invention also concerns novel genes and plasmids.
摘要翻译： 通过修饰编码苏云金芽孢杆菌内毒素的基因可以改善拟南芥中苏云金芽孢杆菌内毒素的表达。 通过用编码不同原毒素的片段替代编码天然原毒素的苏云金芽孢杆菌基因片段来产生嵌合基因。 本文例示的是cryIF / cryI（b）嵌合体，其中天然cryIF原毒素区段已被cryIA（b）原毒素区段取代，以产生在假单胞菌中cryIF毒素的改进的表达。 本发明还涉及新基因和质粒。
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