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公开(公告)号：US20100120596A1
公开(公告)日：2010-05-13
申请号：US12597925
申请日：2008-05-08
IPC分类号： B04B13/00
CPC分类号： B01L3/5021 , B01L3/50215 , B01L2200/026 , B01L2300/087 , B01L2400/0633
摘要： A separation device adapted for separation of a wanted end product from a sample by centrifugation. According to the invention the device comprises: a first, a second and a third compartment; a first fluid connection between the first and second compartments, arranged to be opened and closed by first closing means; a second fluid connection between the second and the third compartments, arranged to be opened and closed by second closing means. The separation device is adapted to be subjected to centrifugation after having been filled with a first density gradient medium in the first compartment, sample in the third compartment and a second density gradient medium or sample in the second compartment, whereby after centrifugation the wanted end product will settle in the second compartment and the first fluid connection and the second fluid connection will be closed by the first closing means and the second closing means respectively.
摘要翻译： 一种分离装置，其适用于通过离心分离所需的最终产物与样品。 根据本发明，该装置包括：第一，第二和第三隔室; 第一和第二隔室之间的第一流体连接，被布置成由第一关闭装置打开和关闭; 第二和第三隔室之间的第二流体连接，被布置成由第二关闭装置打开和关闭。 分离装置适于在填充第一隔室中的第一密度梯度介质，第三隔室中的样品和第二隔室中的第二密度梯度介质或样品之后进行离心，由此在将所需最终产物 将沉积在第二隔室中，并且第一流体连接和第二流体连接将分别由第一关闭装置和第二关闭装置关闭。
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公开(公告)号：US20110124106A1
公开(公告)日：2011-05-26
申请号：US13055800
申请日：2009-07-20
申请人： Gunnar Froman , Henrik Ostlin
发明人： Gunnar Froman , Henrik Ostlin
CPC分类号： B01L3/5021 , B01L3/0217 , B01L2400/0478 , G01N33/491
摘要： A separation device for separating a wanted end product from a liquid sample comprises a container (2) having a first end (5) and a second end (7), the first end having a central orifice (6), a plunger (3) slideably disposed in the container (2) to define a variable liquid receiving chamber between the plunger (3) and the orifice (6), and a permeable partition member (9) mounted to the plunger (3) in a spaced relationship thereto to define a compartment (12) between the partition member (9) and the plunger (3) for receiving liquid density gradient medium (13), wherein liquid may be drawn into the container (2) and expelled therefrom, respectively, through the orifice (6) by movement of the plunger (3) relative to the container (2). A method for separating a wanted end product from a liquid sample is also disclosed.
摘要翻译： 用于将所需最终产品与液体样品分离的分离装置包括具有第一端（5）和第二端（7）的容器（2），所述第一端具有中心孔（6），柱塞（3） 可滑动地设置在容器（2）中以在柱塞（3）和孔口（6）之间限定可变的液体接收室，以及以与其间隔开的关系安装到柱塞（3）上的可渗透分隔构件（9），以限定 分隔构件（9）和用于接收液体密度梯度介质（13）的柱塞（3）之间的隔室（12），其中液体可以被吸入容器（2）并分别通过孔口6排出 ）通过柱塞（3）相对于容器（2）的移动。 还公开了从液体样品中分离所需最终产物的方法。
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公开(公告)号：US20050179901A1
公开(公告)日：2005-08-18
申请号：US10999532
申请日：2004-11-30
申请人： Henrik Ostlin , Lars Eriksson , Magnus Ljungstrom , Tomas Agren
发明人： Henrik Ostlin , Lars Eriksson , Magnus Ljungstrom , Tomas Agren
CPC分类号： G01N21/253 , B01L3/502715 , B01L2300/0654 , B01L2300/0803 , B01L2300/0806 , B01L2400/0409 , G01N21/07 , G01N21/553 , G01N21/554 , G01N35/00069
摘要： A detector arrangement, which comprises a rotatable microfluidic disc and a spectrophotometric detector unit. The arrangement is characterized in that the detector unit is based on surface plasmon resonanc (SPR) and is capable of measuring an analyte within the detection microcavities, each of which is part of a microchannel structure. A microfluidic disc having an axis of symmetry and comprising microchannel structures, each of which has an upstream functional part that is at a shorter radial position than a downstream functional part. The disc is characterized in that there are detection microcavities (DMCs) in at least a part of said microchannel structures, and that each of said DMCs has an SPR surface on an inner wall and a detection detection window extending from the SPR surface to the surface of the disc. The use of the detector arrangement and microfluidic disc described above for determining: a) if the content in one or more of the DMCs is a liquid or a solid state or a gas, and/or b) a feature of an analyte that may be present in a liquid which is present in one or more of the DMCs.
摘要翻译： 一种检测器装置，其包括可旋转的微流体盘和分光光度检测器单元。 该装置的特征在于，检测器单元基于表面等离子体共振（SPR），并且能够测量检测微腔内的分析物，每个分析物是微通道结构的一部分。 一种具有对称轴并且包括微通道结构的微流体盘，每个微通道结构具有位于比下游功能部分更短的径向位置的上游功能部分。 该盘的特征在于，在所述微通道结构的至少一部分中存在检测微腔（DMC），并且每个所述DMC在内壁上具有SPR表面和从SPR表面延伸到表面的检测检测窗口 的光盘。 使用上述检测器装置和微流体盘来确定：a）如果一种或多种DMC中的含量是液体或固体状态或气体，和/或b）分析物的特征可以是 存在于一种或多种DMC中存在的液体中。
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公开(公告)号：US09341595B2
公开(公告)日：2016-05-17
申请号：US13521281
申请日：2011-01-10
发明人： Bengt Bjellqvist , Henrik Ostlin
IPC分类号： G01N27/447
CPC分类号： G01N27/44795 , G01N27/44704
摘要： An electrophoresis gel assembly comprising a gel slab, at least two sample wells and at least one focusing buffer well arranged between two adjacent sample wells and wherein focusing barriers are arranged between the focusing buffer well and the sample wells. A high conductivity focusing buffer can be applied to the focusing buffer well to obtain a lateral focusing of the sample bands during electrophoresis.
摘要翻译： 一种电泳凝胶组件，其包括凝胶板，至少两个样品阱和至少一个聚焦缓冲液，其布置在两个相邻的样品阱之间，并且其中聚焦屏障布置在聚焦缓冲液阱和样品阱之间。 可以将高电导率聚焦缓冲液应用于聚焦缓冲液阱，以在电泳期间获得样品带的侧向聚焦。
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公开(公告)号：US20130168248A1
公开(公告)日：2013-07-04
申请号：US13521281
申请日：2011-01-10
发明人： Bengt Bjellqvist , Henrik Ostlin
IPC分类号： G01N27/447
CPC分类号： G01N27/44795 , G01N27/44704
摘要： An electrophoresis gel assembly comprising a gel slab, at least two sample wells and at least one focusing buffer well arranged between two adjacent sample wells and wherein focusing barriers are arranged between the focusing buffer well and the sample wells. A high conductivity focusing buffer can be applied to the focusing buffer well to obtain a lateral focusing of the sample bands during electrophoresis.
摘要翻译： 一种电泳凝胶组件，其包括凝胶板，至少两个样品阱和至少一个聚焦缓冲液，其布置在两个相邻的样品阱之间，并且其中聚焦屏障布置在聚焦缓冲液阱和样品阱之间。 可以将高电导率聚焦缓冲液应用于聚焦缓冲液阱，以在电泳期间获得样品带的侧向聚焦。
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公开(公告)号：US07295320B2
公开(公告)日：2007-11-13
申请号：US10999532
申请日：2004-11-30
申请人： Henrik Ostlin , Lars Eriksson , Magnus Ljungstrom , Tomas Agren
发明人： Henrik Ostlin , Lars Eriksson , Magnus Ljungstrom , Tomas Agren
IPC分类号： G01N21/55
CPC分类号： G01N21/253 , B01L3/502715 , B01L2300/0654 , B01L2300/0803 , B01L2300/0806 , B01L2400/0409 , G01N21/07 , G01N21/553 , G01N21/554 , G01N35/00069
摘要： A detector arrangement, which comprises a rotatable microfluidic disc and a spectrophotometric detector unit. The arrangement is characterized in that the detector unit is based on surface plasmon resonance (SPR) and is capable of measuring an analyte within the detection microcavities, each of which is part of a microchannel structure.A microfluidic disc having an axis of symmetry and comprising microchannel structures, each of which has an upstream functional part that is at a shorter radial position than a downstream functional part. The disc is characterized in that there are detection microcavities (DMCs) in at least a part of said microchannel structures, and that each of said DMCs has an SPR surface on an inner wall and a detection detection window extending from the SPR surface to the surface of the disc.The use of the detector arrangement and microfluidic disc described above for determining: a) if the content in one or more of the DMCs is a liquid or a solid state or a gas, and/or b) a feature of an analyte that may be present in a liquid which is present in one or more of the DMCs.
摘要翻译： 一种检测器装置，其包括可旋转的微流体盘和分光光度检测器单元。 该装置的特征在于，检测器单元基于表面等离子体共振（SPR），并且能够测量检测微腔内的分析物，每个分析物是微通道结构的一部分。 一种具有对称轴并且包括微通道结构的微流体盘，每个微通道结构具有位于比下游功能部分更短的径向位置的上游功能部分。 该盘的特征在于，在所述微通道结构的至少一部分中存在检测微腔（DMC），并且每个所述DMC在内壁上具有SPR表面和从SPR表面延伸到表面的检测检测窗口 的光盘。 使用上述检测器装置和微流体盘来确定：a）如果一种或多种DMC中的含量是液体或固体状态或气体，和/或b）分析物的特征可以是 存在于一种或多种DMC中存在的液体中。
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公开(公告)号：USD595423S1
公开(公告)日：2009-06-30
申请号：US29329147
申请日：2008-12-10
申请人： Bjorn A. Johansson , Nils Norrman , Henrik Ostlin , Sven Wounder
设计人： Bjorn A. Johansson , Nils Norrman , Henrik Ostlin , Sven Wounder
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