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    Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways
    5.
    发明申请
    Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways 审中-公开
    用于生产允许产生或修饰代谢途径的进化微生物的方法

    公开(公告)号：US20060270013A1

    公开(公告)日：2006-11-30

    申请号：US10546139

    申请日：2004-02-17

    申请人： Michel Chateau ,  Benjamin Gonzalez ,  Isabelle Meynial-Salles ,  Philippe Soucailles ,  Olivier Zink

    发明人： Michel Chateau ,  Benjamin Gonzalez ,  Isabelle Meynial-Salles ,  Philippe Soucailles ,  Olivier Zink

    IPC分类号： C12N15/74 ,  C12N9/10 ,  C12N1/21

    CPC分类号： C12N15/1058 ,  C12N9/0036 ,  C12N15/01 ,  C12P7/18 ,  C12P7/20 ,  C12P7/42 ,  C12P7/52 ,  C12P13/12 ,  C12P33/00

    摘要： The invention relates to a method for the preparation of evolved microorganisms which permit a modification of metabolic pathways, comprising the following steps: a) production of a modified microorganism by genetic modification of initial microorganism cells such as to inhibit the production of or the consumption of a metabolite when the microorganism is cultivated in a defined medium which also affects the capacity of the microorganism for growth, b) culture of the modified microorganisms to induce evolution in said cells, c) selection of the cells of modified microorganisms which are capable of developing in the defined medium. The invention also relates to the strains of evolved microorganisms, the genes evolved which code for evolved proteins and the use of said evolved microorganisms, genes or proteins in a biotransformation method.

    摘要翻译： 本发明涉及一种用于制备能够修饰代谢途径的进化微生物的方法，包括以下步骤：a）通过初始微生物细胞的遗传修饰来产生经修饰的微生物，例如抑制或抑制 微生物在限定培养基中培养的代谢物，其也影响微生物生长的能力，b）培养经修饰的微生物以诱导所述细胞中的进化，c）选择能够发育的修饰的微生物细胞 在定义的介质中。 本发明还涉及进化微生物菌株，进化型编码进化蛋白质的基因以及所述进化微生物，基因或蛋白质在生物转化方法中的应用。