公开(公告)号：US11530434B2

公开(公告)日：2022-12-20

申请号：US16499957

申请日：2018-03-13

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Kentaro Goto ,  Toyohiko Yamauchi ,  Hidenao Yamada

IPC: G06K9/00 ,  C12Q1/24 ,  G01N1/28 ,  G01N15/06 ,  G06T7/00

Abstract: An evaluation method is a method for evaluating a cell mass containing a plurality of aggregated cells, and includes an imaging step of capturing an image of light obtained from the cell mass by irradiating the cell mass with light, an analysis step of setting a reference point for the cell mass included in image data obtained by the imaging step, setting sampling circles centered on the reference point, and determining a parameter for a state of the cell mass based on image data included in regions on the sampling circles, and an evaluation step of evaluating the cell mass based on the parameter.

公开(公告)号：US11630059B2

公开(公告)日：2023-04-18

申请号：US16962024

申请日：2018-11-22

Applicant: HAMAMATSU PHOTONICS K.K. ,  NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE

Inventor： Toyohiko Yamauchi ,  Kentaro Goto ,  Hisayuki Matsui ,  Satoshi Hirakawa ,  Hidenao Yamada

IPC: G01B9/02 ,  G01N21/45 ,  C12M1/34 ,  C12N5/00 ,  H04N5/369

Abstract: An interference image acquisition apparatus includes a light source, a beam splitter, a second reflection mirror, an imager, and a first reflection mirror. A cell is placed on one side of a transparent material, and the first reflection mirror is placed on the other side of the transparent material. In a two-beam interferometer, an optical path difference between an optical path length of a first light beam reflected by the first reflection mirror and an optical path length of a second light beam reflected by the second reflection mirror is set to a coherence length of light output from the light source or less. The imager acquires an interference image in a state in which the cell is placed at a position conjugate to an imaging plane in a first optical system between the imaging plane and the first reflection mirror.

公开(公告)号：US09841414B2

公开(公告)日：2017-12-12

申请号：US14766238

申请日：2014-02-05

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Hidenao Iwai ,  Takuji Kataoka ,  Satoshi Yamamoto ,  Norikazu Sugiyama ,  Toyohiko Yamauchi ,  Kentaro Goto

IPC: G06K9/00 ,  G01N33/483 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49

CPC classification number: G01N33/4833 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4707 ,  G01N2021/479

Abstract: An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn′. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn′ at each time tn.