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    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
    1.
    发明授权
    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor 失效
    细胞群体或子囊的定量方法及其适合的试剂

    公开(公告)号：US5094940A

    公开(公告)日：1992-03-10

    申请号：US376707

    申请日：1989-07-07

    申请人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    发明人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    IPC分类号： G01N33/53 ,  C12Q1/28 ,  C12Q1/34 ,  G01N33/536 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/56972 ,  G01N33/56966 ,  Y10S435/81 ,  Y10S435/967 ,  Y10T436/101666

    摘要： A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behaviour to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.

    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
    2.
    发明授权
    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor 失效
    用于定量细胞群或亚群的方法和适合其的试剂

    公开(公告)号：US4876189A

    公开(公告)日：1989-10-24

    申请号：US927803

    申请日：1986-11-05

    申请人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    发明人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    IPC分类号： G01N33/53 ,  C12Q1/28 ,  C12Q1/34 ,  G01N33/536 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/56972 ,  G01N33/56966 ,  Y10S435/81 ,  Y10S435/967 ,  Y10T436/101666

    摘要： A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behavior to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.

    摘要翻译： 通过将样品与针对要定量的细胞群的特征性表面抗原的标记抗体孵育以形成标记的抗体/抗原复合物，来定量细胞群体或亚群体的方法。 具有已知的，不同的颗粒浓度并且具有与要测定的细胞具有可比较的沉降行为的标准品，并且进一步携带针对标记的抗体或其部分的分子也与标记的抗体一起温育。 将样品溶液的细胞以及标准溶液的颗粒与过量标记的抗体分离出来，并且不仅在细胞上而且在颗粒上测量标记量。 通过将来自样品的测量值与来自标准溶液的测量值的比较，确定样品中待确定的细胞数量。 用于执行该过程的标准品和试剂盒也是本申请的主题。

    Process and reagent for the determination of the luteinizing hormone and monoclonal antibodies suitable thereof
    5.
    发明授权
    Process and reagent for the determination of the luteinizing hormone and monoclonal antibodies suitable thereof 失效
    用于确定适合其的黄体生成激素和单克隆抗体的方法和试剂

    公开(公告)号：US5248593A

    公开(公告)日：1993-09-28

    申请号：US908535

    申请日：1992-06-29

    申请人： Christa Hubner-Parajsz ,  Hartmut Schetters ,  Helmut Lenz ,  Klaus Erler

    发明人： Christa Hubner-Parajsz ,  Hartmut Schetters ,  Helmut Lenz ,  Klaus Erler

    IPC分类号： C07K16/26

    CPC分类号： C07K16/26

    摘要： The present invention provides an immunological process for the determination of the luteinizing hormone (LH), wherein at least one monoclonal antibody is used which is directed against LH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.The present invention also provides a reagent for the determination of the luteinizing hormone, wherein it contains at least one monoclonal antibody which is directed against LH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.Furthermore, the present invention provides a monoclonal antibody against the luteinizing hormone and a process and a hybridoma cell line for producing it.

    摘要翻译： 本发明提供了用于确定黄体生成激素（LH）的免疫学方法，其中使用至少一种针对LH的单克隆抗体和与其他糖蛋白激素交叉反应至少于3％的程度。 本发明还提供了用于测定黄体生成激素的试剂，其中它含有至少一种针对LH的单克隆抗体和与其他糖蛋白激素交叉反应至少于3％的程度。 此外，本发明提供了针对黄体生成激素的单克隆抗体及其制备方法和杂交瘤细胞系。