公开(公告)号：US5248593A

公开(公告)日：1993-09-28

申请号：US908535

申请日：1992-06-29

申请人： Christa Hubner-Parajsz ,  Hartmut Schetters ,  Helmut Lenz ,  Klaus Erler

发明人： Christa Hubner-Parajsz ,  Hartmut Schetters ,  Helmut Lenz ,  Klaus Erler

IPC分类号： C07K16/26

CPC分类号： C07K16/26

摘要： The present invention provides an immunological process for the determination of the luteinizing hormone (LH), wherein at least one monoclonal antibody is used which is directed against LH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.The present invention also provides a reagent for the determination of the luteinizing hormone, wherein it contains at least one monoclonal antibody which is directed against LH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.Furthermore, the present invention provides a monoclonal antibody against the luteinizing hormone and a process and a hybridoma cell line for producing it.

摘要翻译： 本发明提供了用于确定黄体生成激素（LH）的免疫学方法，其中使用至少一种针对LH的单克隆抗体和与其他糖蛋白激素交叉反应至少于3％的程度。 本发明还提供了用于测定黄体生成激素的试剂，其中它含有至少一种针对LH的单克隆抗体和与其他糖蛋白激素交叉反应至少于3％的程度。 此外，本发明提供了针对黄体生成激素的单克隆抗体及其制备方法和杂交瘤细胞系。

公开(公告)号：US4762783A

公开(公告)日：1988-08-09

申请号：US834316

申请日：1986-02-27

申请人： Christa Hubner-Parajsz ,  Hartmut Schetters ,  Helmut Lenz ,  Klaus Erler

发明人： Christa Hubner-Parajsz ,  Hartmut Schetters ,  Helmut Lenz ,  Klaus Erler

IPC分类号： G01N33/53 ,  C07K14/005 ,  C07K14/195 ,  C07K14/575 ,  C07K16/00 ,  C07K16/26 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577 ,  G01N33/532 ,  C12N15/00

CPC分类号： C07K16/26 ,  Y10S435/81 ,  Y10S435/948 ,  Y10S436/808 ,  Y10S436/815

摘要： The present invention provides an immunological process for the determination of the follicle-stimulating hormone (FSH), wherein there is used at least one monoclonal antibody which is directed specifically against FSH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.The present invention also provides a reagent for the determination of the follicle-stimulating hormone, wherein it contains at least one monoclonal antibody which is directed against FSH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.Furthermore, the present invention provides a monoclonal antibody against the follicle-stimulating hormone and a process and a hybridoma cell line for producing it.

摘要翻译： 本发明提供了用于确定促卵泡激素（FSH）的免疫学方法，其中使用至少一种单克隆抗体，其特异性针对FSH并与其他糖蛋白激素交叉反应至少于3 ％。 本发明还提供了一种用于确定促卵泡激素的试剂，其中它含有至少一种针对FSH的单克隆抗体并与其他糖蛋白激素交叉反应至少于3％的程度。 此外，本发明提供了针对促卵泡激素的单克隆抗体和用于制备它的方法和杂交瘤细胞系。

公开(公告)号：US4939082A

公开(公告)日：1990-07-03

申请号：US326885

申请日：1989-03-21

申请人： Kurt W. Naujoks ,  Willie Gerhardt ,  Christa Hubner-Parajsz ,  Karl Wulff ,  Herbert Jungfer ,  Helmut Lenz ,  Winfried Albert ,  August W. Wahlefeld

发明人： Kurt W. Naujoks ,  Willie Gerhardt ,  Christa Hubner-Parajsz ,  Karl Wulff ,  Herbert Jungfer ,  Helmut Lenz ,  Winfried Albert ,  August W. Wahlefeld

IPC分类号： G01N33/537 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12Q1/40 ,  C12R1/91 ,  G01N33/573 ,  G01N33/577

CPC分类号： C12Q1/40 ,  G01N33/573 ,  Y10S435/962 ,  Y10S436/824 ,  Y10S436/828 ,  Y10S530/808 ,  Y10S530/809

摘要： In body fluids containing saliva alpha amylase and pancreatic alpha amylase, pancreatic alpha amylase is determined with a monoclonal antibody which specifically binds but does not inhibit saliva alpha amylase and which has a cross-reactivity of 5% or less toward pancreatic alpha amylase. The monoclonal antibody binds salvia alpha amylase to form a complex which is separated to permit determining pancreatic alpha amylase with an amylase detection system. The complex may be separated by precipitating with a precipitating agent such as an anti-antibody or protein A, or by immobilizing the monoclonal antibody on a solid carrier. The monoclonal antibody is preferably produced by immunizing a host animal with an immunogen containing aluminum hydroxide, Bordatella pertussis and native saliva alpha amylase at least 7 times over a period of at least 9 months, fusing B lymphocytes of the immunized host animal with a transforming agent to produce hybridomas, cloning and culturing the hybridomas, and screening antibodies formed.

摘要翻译： 在含有唾液α淀粉酶和胰腺α淀粉酶的体液中，用特异性结合但不抑制唾液α-淀粉酶的单克隆抗体测定胰腺α淀粉酶，并且对胰腺α淀粉酶具有5％以下的交叉反应性。 单克隆抗体结合salviaα淀粉酶以形成复合物，其被分离以允许用淀粉酶检测系统测定胰腺α淀粉酶。 可以通过用诸如抗体或蛋白质A的沉淀剂沉淀或通过将单克隆抗体固定在固体载体上来分离复合物。 单克隆抗体优选通过用含有氢氧化铝，百日咳杆菌和天然唾液α-淀粉酶的免疫原在至少9个月的时间内至少7次免疫宿主动物来产生，将免疫宿主动物的B淋巴细胞与转化剂 产生杂交瘤，克隆和培养杂交瘤，并筛选形成的抗体。

公开(公告)号：US10663538B2

公开(公告)日：2020-05-26

申请号：US15700124

申请日：2017-09-09

申请人： Wolfgang Bielmeier ,  Gerhard Brinker ,  Swen Campagna ,  Nikolaus Demharter ,  Bernd Erbe ,  Matthias Gebhardt ,  Helmut Lenz ,  Jürgen Nistler ,  Dominik Paul ,  Carsten Prinz ,  Gudrun Ruyters ,  Stephan Stöcker ,  Markus Vester

发明人： Wolfgang Bielmeier ,  Gerhard Brinker ,  Swen Campagna ,  Nikolaus Demharter ,  Bernd Erbe ,  Matthias Gebhardt ,  Helmut Lenz ,  Jürgen Nistler ,  Dominik Paul ,  Carsten Prinz ,  Gudrun Ruyters ,  Stephan Stöcker ,  Markus Vester

IPC分类号： G01R33/24 ,  G01R33/385 ,  G01R33/28

摘要： A method for monitoring a temporal change in a magnetic field in a magnetic resonance device, as well as an evaluation unit, a magnetic resonance device, and a computer program product for performing the method are provided. The method provides that a position-dependent magnetic field distribution that is produced by the plurality of gradient coils is provided with a plurality of monitoring points. In addition, time-dependent gradient values of the plurality of gradient coils are ascertained. Based on position-dependent magnetic field distribution and the time-dependent gradient values, the temporal change in the magnetic field is ascertained. The temporal change in the magnetic field is monitored by comparing the temporal change in the magnetic field with at least one limit value.

公开(公告)号：US20180074138A1

公开(公告)日：2018-03-15

申请号：US15700124

申请日：2017-09-09

申请人： Wolfgang Bielmeier ,  Gerhard Brinker ,  Swen Campagna ,  Nikolaus Demharter ,  Bernd Erbe ,  Matthias Gebhardt ,  Helmut Lenz ,  Jürgen Nistler ,  Dominik Paul ,  Carsten Prinz ,  Gudrun Ruyters ,  Stephan Stöcker ,  Markus Vester

发明人： Wolfgang Bielmeier ,  Gerhard Brinker ,  Swen Campagna ,  Nikolaus Demharter ,  Bernd Erbe ,  Matthias Gebhardt ,  Helmut Lenz ,  Jürgen Nistler ,  Dominik Paul ,  Carsten Prinz ,  Gudrun Ruyters ,  Stephan Stöcker ,  Markus Vester

IPC分类号： G01R33/24 ,  G01R33/385 ,  G01R33/28

CPC分类号： G01R33/24 ,  G01R33/288 ,  G01R33/385

摘要： A method for monitoring a temporal change in a magnetic field in a magnetic resonance device, as well as an evaluation unit, a magnetic resonance device, and a computer program product for performing the method are provided. The method provides that a position-dependent magnetic field distribution that is produced by the plurality of gradient coils is provided with a plurality of monitoring points. In addition, time-dependent gradient values of the plurality of gradient coils are ascertained. Based on position-dependent magnetic field distribution and the time-dependent gradient values, the temporal change in the magnetic field is ascertained. The temporal change in the magnetic field is monitored by comparing the temporal change in the magnetic field with at least one limit value.

公开(公告)号：US08664007B2

公开(公告)日：2014-03-04

申请号：US11260938

申请日：2005-10-28

申请人： Ursula Klause ,  Helmut Lenz ,  Christine Markert-Hahn

发明人： Ursula Klause ,  Helmut Lenz ,  Christine Markert-Hahn

IPC分类号： G01N33/543

CPC分类号： G01N33/543 ,  G01N33/6854

摘要： The invention concerns a method for determining antigen-specific antibodies of a particular immunoglobulin class in a sample by means of an immunoassay in an array format in which various binding partners Bnx are bound on different discrete areas on a support where Bnx in each case contain the various antigens that are able to specifically bind to the antibodies to be detected, by incubating the support with the sample and a binding partner B2 which carries a label and subsequently detecting the label on the respective discrete areas wherein B2 specifically binds antibodies of a certain immunoglobulin class that have been bound in an antigen-specific manner.

摘要翻译： 本发明涉及通过以阵列格式的免疫测定来测定样品中特定免疫球蛋白类别的抗原特异性抗体的方法，其中各种结合配偶体Bnx结合在支架上的不同离散区域上，其中每种情况下Bnx包含 能够通过将载体与样品一起孵育的各种抗原和携带标记的结合伴侣B2，随后检测各个离散区域上的标记，其中B2特异性结合某种免疫球蛋白的抗体 已经以抗原特异性方式结合的类。

公开(公告)号：US20080012636A1

公开(公告)日：2008-01-17

申请号：US11629442

申请日：2005-06-14

申请人： Helmut Lenz

发明人： Helmut Lenz

IPC分类号： H03F1/36

CPC分类号： G01R33/3852

摘要： A method for controlling an electric amplifier is provided. The method includes monitoring a closed-loop control signal of a regulator, which triggers an end stage of the amplifier; adjusting the closed-loop control signal as a function of an output signal of the end stage; comparing the closed-loop control signal with a reference signal; triggering the end stage when an amplitude limit value and a period length limit value are exceeded or undershot by the amplitude value and period length value, respectively, of the closed-loop control signal; and blocking an input side and/or the regulator when the end stage is triggered.

摘要翻译： 提供了一种用于控制电放大器的方法。 该方法包括监视调节器的闭环控制信号，其触发放大器的末级; 根据终端的输出信号调整闭环控制信号; 将闭环控制信号与参考信号进行比较; 当分别超过或低于闭环控制信号的振幅值和周期长度值的振幅限制值和周期长度限制值时，触发结束级; 并且当终端被触发时阻塞输入侧和/或调节器。

公开(公告)号：US20070262892A1

公开(公告)日：2007-11-15

申请号：US11784047

申请日：2007-04-05

申请人： Helmut Lenz

发明人： Helmut Lenz

IPC分类号： H03M3/00

CPC分类号： H03F3/2173

摘要： A method for generating at least one modulator input signal from at least one regulator signal is provided. The method comprising: generating at least one dither signal based on the periodic output of elements of a discrete dither sequence of the basic form DF=0, 1, 2 . . . 2(m−n)−1; and adding the at least one dither signal to the regulator signal. The regulator signal comprises a digital regulator signal with a length of m bits, and the modulator input signal comprises a digital modulator input signal with a length of n bits, where m>n.

摘要翻译： 提供了一种用于从至少一个调节器信号产生至少一个调制器输入信号的方法。 该方法包括：基于基本形式DF = 0,1,2的离散抖动序列的元素的周期性输出来产生至少一个抖动信号。 。 。 2（m-n）-1; 以及将所述至少一个抖动信号添加到所述调节器信号。 调节器信号包括长度为m位的数字调节器信号，并且调制器输入信号包括长度为n位的数字调制器输入信号，其中m> n。

公开(公告)号：US07091632B2

公开(公告)日：2006-08-15

申请号：US10266293

申请日：2002-10-08

申请人： Helmut Lenz

发明人： Helmut Lenz

IPC分类号： H02J3/24

CPC分类号： H02J3/1814 ,  H02J9/06 ,  Y02E40/18

摘要： A stabilization circuit wherein voltages of the secondary winding of a transformer are stabilized with respect to mains voltage fluctuations and load fluctuations, an auxiliary voltage is derived either from a secondary winding of the transformer or an auxiliary transformer. Together with the mains voltage portion of this auxiliary voltage acquired by pulse-width modulation forms the primary voltage of the transformer such that the desired stabilization is achieved.

公开(公告)号：US6060285A

公开(公告)日：2000-05-09

申请号：US500596

申请日：1990-03-22

申请人： Helmut Lenz ,  Ulrich Weidle

发明人： Helmut Lenz ,  Ulrich Weidle

IPC分类号： C07K16/46 ,  C12N5/10 ,  C12N15/09 ,  C12N15/13 ,  C12P21/08 ,  C12R1/19 ,  C12R1/91 ,  C07H15/12 ,  C12N5/00 ,  C12N15/00 ,  C12P21/06

CPC分类号： C07K16/468 ,  Y10S435/972

摘要： For the production of hetero-bispecific monoclonal antibodies at least the genes for the light chain and for the variable part of the heavy chain are isolated from a hybridoma cell line which secretes an antibody with a desired specificity and they are inserted into a eukaryotic plasmid vector which contains a marker capable of selection together with a strong promoter, this expression vector is transfected into a hybridoma cell line which secretes antibodies with a second desired specificity, the cell line is cultured, the antibodies are obtained and the bispecific antibody is isolated.

摘要翻译： 为了生产异双特异性单克隆抗体，至少将轻链基因和重链可变部分从杂交瘤细胞系中分离出来，所述杂交瘤细胞系将具有所需特异性的抗体分泌，并将其插入真核质粒载体 其包含能够与强启动子一起选择的标记，将该表达载体转染到以第二所需特异性分泌抗体的杂交瘤细胞系中，培养细胞系，获得抗体并分离双特异性抗体。