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公开(公告)号：US20070059834A1
公开(公告)日：2007-03-15
申请号：US11427474
申请日：2006-06-29
IPC分类号： C12N15/87
CPC分类号： C12N15/87 , A61N1/0412 , A61N1/327
摘要： Disclosed is a method for treating biological material via electrical current, in which the biological material is added to a small volume of a buffer solution having relative high ionic strength. A strong electrical field is generated in the buffer solution by a high voltage pulse having a preset duration. The biological material is added to at most 50 μl of a buffer solution with an ionic strength of at least 100 mmol/l. By at least one voltage pulse having a preset duration of at least 10 μs, an electrical field with a field strength of at least 1 kV/cm is generated in the buffer solution. The voltage pulse is hereby interrupted at least once for a duration of at least 100 μs and is then again continued.
摘要翻译： 公开了一种通过电流处理生物材料的方法，其中将生物材料加入到具有相对高的离子强度的小体积的缓冲溶液中。 通过具有预设持续时间的高电压脉冲在缓冲溶液中产生强电场。 将生物材料加入到至多50ul的离子强度为至少100mmol / l的缓冲溶液中。 通过具有至少10μs的预设持续时间的至少一个电压脉冲，在缓冲溶液中产生场强至少为1kV / cm的电场。 电压脉冲至少中断至少一次持续至少100次的脉冲，然后再次继续。
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公开(公告)号：US07700357B2
公开(公告)日：2010-04-20
申请号：US11427474
申请日：2006-06-29
IPC分类号： C12N15/87
CPC分类号： C12N15/87 , A61N1/0412 , A61N1/327
摘要： Disclosed is a method for treating biological material via electrical current, in which the biological material is added to a small volume of a buffer solution having relative high ionic strength. A strong electrical field is generated in the buffer solution by a high voltage pulse having a preset duration.The biological material is added to at most 50 μl of a buffer solution with an ionic strength of at least 100 mmol/l. By at least one voltage pulse having a preset duration of at least 10 μs, an electrical field with a field strength of at least 1 kV/cm is generated in the buffer solution. The voltage pulse is hereby interrupted at least once for a duration of at least 100 μs and is then again continued.
摘要翻译： 公开了一种通过电流处理生物材料的方法，其中将生物材料加入到具有相对高的离子强度的小体积的缓冲溶液中。 通过具有预设持续时间的高电压脉冲在缓冲溶液中产生强电场。 将生物材料加入至多50μl具有至少100mmol / l的离子强度的缓冲溶液。 通过具有至少10μs的预设持续时间的至少一个电压脉冲，在缓冲溶液中产生场强至少为1kV / cm的电场。 电压脉冲因此在至少100μs的持续时间中断至少一次，然后再次继续。
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公开(公告)号：US09128072B2
公开(公告)日：2015-09-08
申请号：US13121581
申请日：2009-09-24
申请人： Edgar Dieβel , Ingmar Dorn , Michael Habig , Mike Küster , Klaus Ochmann
发明人： Edgar Dieβel , Ingmar Dorn , Michael Habig , Mike Küster , Klaus Ochmann
CPC分类号： G01N35/028 , G01N2035/00306 , G01N2035/00326 , G01N2035/1051 , Y10T436/11
摘要： The present invention relates to an apparatus and a method for automatically performing chemical, biochemical and biological analyses using the apparatus. The apparatus comprises: a plurality of subsystems for each performing different operations on microtiter plates; a transporting subsystem for automated movement of microtiter plates in three directions X, Y and Z in order to transport plates from one subsystem to another subsystem within the apparatus; a liquid handler comprising at least two liquid handling probes, wherein said liquid handler is integrated in the transporting subsystem; an insertion/removal subsystem for inserting, removing and storing microtiter plates and equipment for sampling and dilution operations, wherein said insertion/removal subsystem is mechanically operated, without the need for electrical components implemented in that subsystem.
摘要翻译： 本发明涉及使用该装置自动进行化学，生物化学和生物分析的装置和方法。 该装置包括：多个子系统，用于每个在微量滴定板上执行不同的操作; 运输子系统，用于在三个方向X，Y和Z上自动移动微量滴定板，以便将板从一个子系统传送到设备内的另一个子系统; 液体处理器，其包括至少两个液体处理探针，其中所述液体处理器集成在所述输送子系统中; 用于插入，移除和存储用于取样和稀释操作的微量滴定板和设备的插入/移除子系统，其中所述插入/移除子系统是机械操作的，而不需要在该子系统中实现电气部件。
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公开(公告)号：US07678564B2
公开(公告)日：2010-03-16
申请号：US10505149
申请日：2003-02-20
CPC分类号： C12M35/02
摘要： The invention relates to a container 20, 30 for receiving an aqueous solution, which is formed at least partially by an outer limit 21 forming an inner chamber 22, 32 for receiving the solution, and which comprises at least one area which acts as an electrode 25, 26, 33, 34 when an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrode 25, 26, 33, 34 is made of a conductive synthetic material at least based on a plastic material which is doped with at least one conductive substance. A container 20, 30 of the above-mentioned kind is created this way, which is simple and economical to produce and also, for example, enables an efficient transfection of living cells by means of electroporation or an effective electrofusion.
摘要翻译： 本发明涉及用于接收水溶液的容器20,30，其至少部分地由形成用于接收溶液的内室22,32的外限制21形成，并且包括至少一个充当电极的区域 25,26,33,34，当施加电压并发生随后的放电时，其中至少一个电极25,26,33,34由导电合成材料制成，至少基于掺杂有 至少一种导电物质。 以这种方式产生上述类型的容器20,30，其生产简单和经济，并且还例如能够通过电穿孔或有效的电融合有效转染活细胞。
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5.发明申请公开(公告)号：US20060087522A1
公开(公告)日：2006-04-27
申请号：US11079192
申请日：2005-03-15
IPC分类号： B41J2/01
CPC分类号： C12M23/12 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00585 , B01J2219/00599 , B01J2219/00689 , B01J2219/00713 , B01J2219/00743 , B01L3/50855 , B01L2300/0645 , B01L2300/0829 , C12M35/02
摘要： The invention concerns a container 1 with chambers 2 which each comprise at least one pair of electrodes including a first 4 and a second electrode 5 for the application of electric voltage for generating an electric field within one chamber 2. At least two first electrodes 4 of different chambers 3 are conductively coupled and at least one second electrode 5 of said chamber 2 is separately conductively connectable. The invention further concerns a method for manufacturing said container 1 as well as a device for electrically contacting at least one of said containers 1.
摘要翻译： 本发明涉及具有腔室2的容器1，每个腔室2包括至少一对电极，其包括用于在一个腔室2内产生电场的电压施加的第一电极5和第二电极5。 不同腔室3的至少两个第一电极4被导电耦合，并且所述腔室2的至少一个第二电极5可分开导电连接。 本发明还涉及用于制造所述容器1的方法以及用于电接触所述容器1中的至少一个的装置。
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6.发明授权公开(公告)号：US08101401B2
公开(公告)日：2012-01-24
申请号：US11079192
申请日：2005-03-15
CPC分类号： C12M23/12 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00585 , B01J2219/00599 , B01J2219/00689 , B01J2219/00713 , B01J2219/00743 , B01L3/50855 , B01L2300/0645 , B01L2300/0829 , C12M35/02
摘要： The invention concerns a container 1 with chambers 2 which each comprise at least one pair of electrodes including a first 4 and a second electrode 5 for the application of electric voltage for generating an electric field within one chamber 2. At least two first electrodes 4 of different chambers 3 are conductively coupled and at least one second electrode 5 of said chamber 2 is separately conductively connectable. The invention further concerns a method for manufacturing said container 1 as well as a device for electrically contacting at least one of said containers 1.
摘要翻译： 本发明涉及具有腔室2的容器1，每个腔室2包括至少一对电极，其包括用于在一个腔室2内产生电场的电压施加的第一电极5和第二电极5.至少两个第一电极4 不同的腔室3被导电耦合，并且所述腔室2的至少一个第二电极5是可分开导电连接的。 本发明还涉及用于制造所述容器1的方法以及用于电接触所述容器1中的至少一个的装置。
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公开(公告)号：US20110269239A1
公开(公告)日：2011-11-03
申请号：US13121581
申请日：2009-09-24
申请人： Edgar Diessel , Ingmar Dorn , Michael Habig , Mike Küster , Klaus Ochmann
发明人： Edgar Diessel , Ingmar Dorn , Michael Habig , Mike Küster , Klaus Ochmann
IPC分类号： G01N35/00
CPC分类号： G01N35/028 , G01N2035/00306 , G01N2035/00326 , G01N2035/1051 , Y10T436/11
摘要： The present invention relates to an apparatus and a method for automatically performing chemical, biochemical and biological analyses.
摘要翻译： 本发明涉及一种用于自动执行化学，生物化学和生物学分析的装置和方法。
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公开(公告)号：US20050064578A1
公开(公告)日：2005-03-24
申请号：US10505149
申请日：2003-02-20
CPC分类号： C12M35/02
摘要： The invention relates to a container 20, 30 for receiving an aqueous solution, which is formed at least partially by an outer limit 21 forming an inner chamber 22, 32 for receiving the solution, and which comprises at least one area which acts as an electrode 25, 26, 33, 34 when an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrode 25, 26, 33, 34 is made of a conductive synthetic material at least based on a plastic material which is doped with at least one conductive substance. A container 20, 30 of the above-mentioned kind is created this way, which is simple and economical to produce and also, for example, enables an efficient transfection of living cells by means of electroporation or an effective electrofusion.
摘要翻译： 本发明涉及用于接收水溶液的容器20,30，其至少部分地由形成用于接收溶液的内室22,32的外限制21形成，并且包括至少一个充当电极的区域 25,26,33,34，当施加电压并发生随后的放电时，其中至少一个电极25,26,33,34由导电合成材料制成，至少基于掺杂在其上的塑料材料 至少一种导电物质。 以这种方式产生上述类型的容器20,30，其生产简单和经济，并且还例如能够通过电穿孔或有效的电融合有效转染活细胞。
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