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1.发明授权Biological support for cell cultures constituted by plasma proteins coagulated by thrombin, its use in the preparation of keratocyte cultures, their recovery and their transport for therapeutic purposes 失效由凝血酶凝结的血浆蛋白构成的细胞培养物的生物支持，其用于制备角化细胞培养物，其回收及其用于治疗目的的运输
公开(公告)号：US5474770A
公开(公告)日：1995-12-12
申请号：US850260
申请日：1992-03-12
申请人： Herve Broly , Vincent Ronfard
发明人： Herve Broly , Vincent Ronfard
CPC分类号： A61L27/60 , C12N5/0068 , C12N2533/56 , Y10S623/915
摘要： The present invention relates to a biological support for cell cultures formed by the coagulated mixture of a concentrate of plasma proteins and thrombin.The protein concentrate is obtained by precipitating fresh plasma with ethanol and contains balanced proportions of fibrinogen, Factor XIII and fibronectin. The thrombin concentration is adjusted to obtain the desired consistency of the support coagulated in the form of a film.The biological support is preferably used for preparing a culture of keratinocytes, recovering them in the form of a reconstituted tissue and transporting same. The reconstituted tissue is thus particularly suitable for use as a graft.
摘要翻译： 本发明涉及由血浆蛋白和凝血酶浓缩物的凝固混合物形成的细胞培养物的生物支持体。 蛋白浓缩物通过用乙醇沉淀新鲜血浆并含有平衡比例的纤维蛋白原，因子XIII和纤连蛋白获得。 调节凝血酶浓度以获得以膜形式凝结的载体的期望稠度。 生物载体优选用于制备角质形成细胞的培养物，以重组组织的形式回收它们并进行运输。 因此，重组组织特别适合用作移植物。
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公开(公告)号：US20060134093A1
公开(公告)日：2006-06-22
申请号：US10526854
申请日：2003-09-05
申请人： Vincent Ronfard
发明人： Vincent Ronfard
IPC分类号： A61K38/48
CPC分类号： C12N5/0698 , A61K35/12 , A61K35/36 , A61K38/00 , A61K38/57 , A61L27/225 , A61L27/3813 , A61L27/60 , C12N5/0068 , C12N2502/094 , C12N2502/1323 , C12N2533/56 , A61K2300/00
摘要： The present invention relates to fibrin cell supports for cell cultures formed by the mixture of plasma proteins including fibrinogen and thrombin. The fibrin cell supports are preferably used for preparing a culture of cells such as keratinocytes, recovering the culture in the form of a reconstituted tissue, and transporting same. The reconstituted tissue is particularly suitable for use as a skin graft.
摘要翻译： 本发明涉及由血浆蛋白质（包括纤维蛋白原和凝血酶）的混合物形成的细胞培养物的纤维蛋白细胞支持体。 纤维蛋白细胞支持体优选用于制备细胞培养物，例如角质形成细胞，以复原组织的形式回收培养物并进行转运。 重组组织特别适合用作皮肤移植物。
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公开(公告)号：US06673603B2
公开(公告)日：2004-01-06
申请号：US09943114
申请日：2001-08-30
IPC分类号： C12N500
CPC分类号： A61L27/3804 , A61K35/12 , A61L15/40 , A61L26/0057 , A61L27/3633 , C12N5/0656 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2510/00
摘要： The present invention provides the use and composition of matter of angiogenic or other growth factors expressed by combining various types and stages of differentiation of allogeneic human cell strains or lines in unencapsulated pastes (mixed with or applied to extracellular matrix material or synthetic biocompatible substances) to be temporarily applied to wounds or defects in the skin or other tissues for the restoration of blood supplying connective tissue to enable organ-specific cells to reestablish organ integrity as well as to inhibit excessive scar formation.
摘要翻译： 本发明提供血管生成或其他生长因子的用途和组成，其通过将未包封的糊状物（与细胞外基质材料或合成的生物相容性物质混合或应用）中的同种异体人细胞株或细胞系的各种类型和阶段的分化相结合而表达， 临时应用于皮肤或其他组织中的伤口或缺陷，用于恢复供血结缔组织，以使器官特异性细胞重新建立器官完整性以及抑制过度的瘢痕形成。
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4.发明授权公开(公告)号：US07824913B2
公开(公告)日：2010-11-02
申请号：US09523809
申请日：2000-03-13
申请人： Michael P. Murphy , Vincent Ronfard
发明人： Michael P. Murphy , Vincent Ronfard
CPC分类号： C12N5/0698 , A61K35/12 , C12N2500/90 , C12N2502/094 , C12N2502/1323
摘要： Cultured tissue constructs comprising cultured cells and endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. Some tissue constructs of the invention are comprised of multiple cell layers or more than one cell type. The tissue constructs of the invention have morphological features and functions similar to tissues their cells are derived and their strength makes them easily handleable. Preferred cultured tissue constructs of the invention are prepared in defined media, that is, without the addition of chemically undefined components.
摘要翻译： 培养的组织构建体包含培养的细胞和内源性产生的细胞外基质组分，而不需要外源基质组分或网络支持物或支架构件。 本发明的一些组织构建体由多个细胞层或多于一种细胞类型组成。 本发明的组织构建体具有与其细胞衍生的组织相似的形态特征和功能，并且它们的强度使得它们易于处理。 在限定的培养基中制备本发明优选的培养的组织构建体，即不添加化学未定义的组分。
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公开(公告)号：US20060240555A1
公开(公告)日：2006-10-26
申请号：US11362379
申请日：2006-02-24
申请人： Vincent Ronfard
发明人： Vincent Ronfard
CPC分类号： C12N5/0698 , A61K35/12 , A61K35/36 , A61K38/00 , A61K38/57 , A61L27/225 , A61L27/3813 , A61L27/60 , C12N5/0068 , C12N2502/094 , C12N2502/1323 , C12N2533/56 , A61K2300/00
摘要： The present invention relates to fibrin cell supports for cell cultures formed by the mixture of plasma proteins including fibrinogen and thrombin. The fibrin cell supports are preferably used for preparing a culture of cells such as keratinocytes, recovering the culture in the form of a reconstituted tissue, and transporting same. The reconstituted tissue is particularly suitable for use as a skin graft.
摘要翻译： 本发明涉及由血浆蛋白质（包括纤维蛋白原和凝血酶）的混合物形成的细胞培养物的纤维蛋白细胞支持体。 纤维蛋白细胞支持体优选用于制备细胞培养物，例如角质形成细胞，以复原组织的形式回收培养物并进行转运。 重组组织特别适合用作皮肤移植物。
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6.发明申请公开(公告)号：US20080108134A1
公开(公告)日：2008-05-08
申请号：US11932052
申请日：2007-10-31
申请人： Michael Murphy , Vincent Ronfard
发明人： Michael Murphy , Vincent Ronfard
IPC分类号： C12N5/06
CPC分类号： C12N5/0698 , A61K35/12 , C12N2500/90 , C12N2502/094 , C12N2502/1323
摘要： Cultured tissue constructs comprising cultured cells and endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. Some tissue constructs of the invention are comprised of multiple cell layers or more than one cell type. The tissue constructs of the invention have morphological features and functions similar to tissues their cells are derived and their strength makes them easily handleable. Preferred cultured tissue constructs of the invention are prepared in defined media, that is, without the addition of chemically undefined components.
摘要翻译： 包含培养的细胞和内源产生的细胞外基质组分的培养的组织构建体，而不需要外源基质组分或网络支持物或支架构件。 本发明的一些组织构建体由多个细胞层或多于一种细胞类型组成。 本发明的组织构建体具有与其细胞衍生的组织相似的形态特征和功能，并且它们的强度使得它们易于处理。 在限定的培养基中制备本发明优选的培养的组织构建体，即不添加化学未定义的组分。
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公开(公告)号：US20080009064A1
公开(公告)日：2008-01-10
申请号：US11774275
申请日：2007-07-06
IPC分类号： C12N5/00
CPC分类号： C12N5/0075 , C12N2531/00 , C12N2533/30 , C12N2539/10
摘要： The invention relates to compositions and methods useful for cell culture in which cell adherence and release of cultured cells can be performed in a temperature-responsive manner.
摘要翻译： 本发明涉及用于细胞培养的组合物和方法，其中细胞粘附和培养细胞的释放可以以温度响应的方式进行。
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公开(公告)号：US20070243158A1
公开(公告)日：2007-10-18
申请号：US11761893
申请日：2007-06-12
申请人： Vincent Ronfard , Alan Tuck , Leon Wilkins
发明人： Vincent Ronfard , Alan Tuck , Leon Wilkins
CPC分类号： C12N5/0629 , A61K8/99 , A61K35/12 , A61Q19/08 , C12N2502/094 , C12N2502/1323
摘要： The invention is directed to compositions containing growth agents synthesized from cultured cells from skin. Skin cells such as keratinocytes and dermal fibroblasts are cultured in vitro in cell medium and in the course of culture the cultured cells synthesize and secrete agents into the cell medium. The medium containing agents are collected and incorporated into pharmaceutical or cosmetic preparations to treat an individual. The preparation is applied and has a rejuvenating effect on the cells and tissue.
摘要翻译： 本发明涉及含有从皮肤培养的细胞合成的生长剂的组合物。 皮肤细胞如角质形成细胞和真皮成纤维细胞在细胞培养基中体外培养，培养过程中培养的细胞合成并将细胞分泌到细胞培养基中。 收集含有培养基的试剂，并将其并入药物或化妆品制剂中以治疗个体。 应用制剂，对细胞和组织具有恢复活力。
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