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公开(公告)号：US5731176A
公开(公告)日：1998-03-24
申请号：US28463
申请日：1993-03-09
IPC分类号： C12N15/53 , C07H21/04 , C12N20060101 , C12N1/21 , C12N9/34 , C12N9/78 , C12N15/09 , C12N15/31 , C12N15/55 , C12N15/60 , C12P13/02 , C12R1/01 , C12R1/19 , C12N1/00 , C12N5/00 , C12N15/63
摘要： The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.
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公开(公告)号：US5334519A
公开(公告)日：1994-08-02
申请号：US777641
申请日：1991-10-15
申请人： Hideaki Yamada , Toru Nagasawa
发明人： Hideaki Yamada , Toru Nagasawa
IPC分类号： C07C231/06 , C08J9/00 , C12N9/78 , C12P13/02 , C12P17/00 , C12P17/02 , C12P17/04 , C12P17/10 , C12P17/12 , C12R1/01 , C12N1/20 , C12N1/38
CPC分类号： C08J9/0023 , C12N9/78 , C12P13/02 , C08J2325/00
摘要： In a process for biological production of an amide wherein a nitrile is hydrated into a corresponding amide by the action of a nitrile hydratase originated in a microorganism, the improvement which comprises the use of nitrile hydratase which is obtained by culturing a microorganism of the species Rhodococcus rhodochrous in the presence of a cobalt ion.Aromatic nitriles such as a cyanopyridine as well as aliphatic nitrile such as acrylonitrile are hydrated into the corresponding amides.
摘要翻译： 在生物制备酰胺的方法中，其中腈通过起源于微生物的腈水合酶的作用被水合成相应的酰胺，其改进包括使用通过培养物种红球菌的微生物获得的腈水合酶 在钴离子存在下为紫红色。 芳族腈如氰基吡啶以及脂族腈如丙烯腈水合成相应的酰胺。
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3.发明授权Method of culturing a strain of Rhodococcus rhodochrous having nitrile hydratase activity 失效培养具有硝酸盐水解酶活性的RHODOCOCCUS RHODOCHRO的菌株的方法
公开(公告)号：US5089411A
公开(公告)日：1992-02-18
申请号：US417259
申请日：1989-10-05
申请人： Hideaki Yamada , Toru Nagasawa
发明人： Hideaki Yamada , Toru Nagasawa
CPC分类号： C12N9/78
摘要： Cells of bacteria of the species Rhodococcus rhodochrous having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one of urea and its derivatives and cobalt ion in the preparation of cells of bacteria having nitrile hydratase activity by cultivating Rhodococcus rhodochrous bacteria capable of producing nitrile hydratase.
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4.发明授权Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效编码具有腈水合酶活性的多肽的基因，含有该基因的转化体和使用转化体生产酰胺的方法
公开(公告)号：US5789211A
公开(公告)日：1998-08-04
申请号：US463470
申请日：1995-06-05
CPC分类号： C12N9/80
摘要： The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.
摘要翻译： 本发明涉及一种源自绿脓假单胞菌B23株的基因，该基因编码具有腈水合酶活性的能够将腈水合至酰胺的多肽。 本发明还涉及含有该基因的重组DNA和用重组DNA转化的转化体。 本发明还涉及使用腈水合酶使用转化体和酰胺生产腈水合酶的方法。
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5.发明授权Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效编码具有腈水合酶活性的多肽的基因，含有该基因的转化体和使用转化体生产酰胺的方法
公开(公告)号：US5648256A
公开(公告)日：1997-07-15
申请号：US233146
申请日：1994-04-22
CPC分类号： C12N9/80
摘要： The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.
摘要翻译： 本发明涉及一种源自绿脓假单胞菌B23株的基因，该基因编码具有腈水合酶活性的能够将腈水合至酰胺的多肽。 本发明还涉及含有该基因的重组DNA和用重组DNA转化的转化体。 本发明还涉及使用腈水合酶使用转化体和酰胺生产腈水合酶的方法。
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6.发明授权Manufacture of optically active 2-phenylpropionic acid and 2-phenylpropionamide from the nitrile using Rhodococcus equi 失效使用红球菌等从腈制备光学活性的2-苯基丙酸和2-苯基丙酰胺
公开(公告)号：US5258305A
公开(公告)日：1993-11-02
申请号：US849768
申请日：1992-03-12
申请人： Hideaki Yamada , Toru Nagasawa
发明人： Hideaki Yamada , Toru Nagasawa
CPC分类号： C12R1/01 , C12P13/02 , C12P41/006 , C12P7/40 , Y10S435/843
摘要： The present invention provides a method of obtaining optically active 2-phenylpropionic acid and 2-phenylpropionamide such as S-(+)-2-phenylpropionic acid, R-(-)-2-phenylpropionamide, and R-(-)-2-phenylpropionic acid from S-2-phenylpropionitrile or R,S-2-phenylpropionamide using a bacterium belonging to Rhodococcus equi.Optically active 2-phenylpropionic acid and 2-phenylpropionamide are recovered in high yield and in quantities according to the method of the invention.
摘要翻译： 本发明提供了获得光学活性的2-苯基丙酸和2-苯基丙酰胺如S - （+） - 2-苯基丙酸，R - （ - ） - 2-苯基丙酰胺和R - （ - ） - 2- 苯基丙酸由S-2-苯基丙腈或R，S-2-苯基丙酰胺使用属于红球菌属的细菌。 根据本发明的方法，以高产率和数量回收光学活性的2-苯基丙酸和2-苯基丙酰胺。
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公开(公告)号：US5135858A
公开(公告)日：1992-08-04
申请号：US659878
申请日：1991-02-22
申请人： Hideaki Yamada , Toru Nagasawa , Tetsuji Nakamura
发明人： Hideaki Yamada , Toru Nagasawa , Tetsuji Nakamura
CPC分类号： C12P17/10 , C12P17/00 , C12P17/04 , C12P7/40 , Y10S435/822
摘要： An improved biological conversion of a nitrile such as acrylonitrile or a cyanopyridine into the corresponding carboxylic acid such as acrylic acid or a nicotinic acid by the action upon the nitrile of a nitrilase enzyme, in which the improvement resides in the use as the source of the enzyme of a microorganism of Rhodococcus, such as Rh. rhodochrous J-1, FERM BP-1478, which is cultured in the presence of a lactam compound.
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8.发明授权DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效编码具有腈水合酶活性的多肽的DNA片段，含该基因的转化体和使用转化体生产酰胺的方法
公开(公告)号：US5753472A
公开(公告)日：1998-05-19
申请号：US461836
申请日：1995-06-05
CPC分类号： C12P13/02 , C12N9/78 , Y10S435/874
摘要： The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.
摘要翻译： 本发明公开了源自玫瑰色红球菌J-1的两种腈水合酶的α-和β-亚基的氨基酸序列和核苷酸序列。 将编码腈水合酶的DNA片段插入表达载体中，并使用重组载体进行转化。 与常规使用的微生物相比，转化体含有多个拷贝的基因，可以产生更高水平的腈水合酶。
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公开(公告)号：US07666635B2
公开(公告)日：2010-02-23
申请号：US11931639
申请日：2007-10-31
申请人： Karen Tracey Robins , Toru Nagasawa
发明人： Karen Tracey Robins , Toru Nagasawa
CPC分类号： C12N9/88 , C12N9/78 , C12P13/02 , C12R1/01 , C12R1/03 , Y10S435/822 , Y10S435/829
摘要： A novel biotechnological process for the preparation of nitriles, starting from amides, is described. Micro-organisms of the genus Amycolatopsis, Actinomadura or Rhodococcus are employed for this process.
摘要翻译： 描述了从酰胺开始制备腈的新型生物技术方法。 该方法采用无菌纲，放线菌属或红球菌属的微生物。
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公开(公告)号：US06444451B1
公开(公告)日：2002-09-03
申请号：US09463203
申请日：2000-03-27
申请人： Karen Tracey Robins , Toru Nagasawa
发明人： Karen Tracey Robins , Toru Nagasawa
IPC分类号： C12P104
CPC分类号： C12N9/88 , C12N9/78 , C12P13/02 , C12R1/01 , C12R1/03 , Y10S435/822 , Y10S435/829
摘要： A novel biotechnological process for the preparation of nitriles, starting from amides, is described. Microorganisms of the genus Amycolatopsis, Actinomadura or Rhodococcus are employed for this process.
摘要翻译： 描述了从酰胺开始制备腈的新型生物技术方法。 该过程采用无菌纲，放线菌属或红球菌属的微生物。
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