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1.发明授权Test kit for intracellular introduction of protein and/or peptide and method of intracellular introduction 有权用于细胞内引入蛋白质和/或肽的测试试剂盒和细胞内引入方法公开(公告)号：US07252960B2
公开(公告)日：2007-08-07
申请号：US10671881
申请日：2003-09-29
摘要： A test kit for intracellular introduction of a protein and/or peptide and a method of such intracellular introduction, make it possible for a protein and/or peptide to be introduced into cells efficiently and in an expedient manner while preventing the function of the introduction target protein and/or peptide from being reduced. The above test kit comprises a container containing a cationic group-containing polymer and a container containing a reagent for coupling or binding the cationic group-containing polymer to the protein and/or peptide, or comprises a container containing a cationized conjugate to be coupled to the protein and/or peptide. The above method comprises using a conjugate generated by coupling or binding a cationic group-containing polymer to a protein and/or peptide as a carrier for the introduction target protein and/or peptide.
摘要翻译： 用于细胞内引入蛋白质和/或肽的测试试剂盒和这种细胞内引入的方法使得蛋白质和/或肽可以有效地和方便地引入细胞中，同时防止引入靶的功能 蛋白质和/或肽被还原。 上述测试试剂盒包括含有含阳离子基团的聚合物的容器和含有用于将含阳离子基团的聚合物与蛋白质和/或肽偶联或结合的试剂的容器，或包含含有阳离子化缀合物的容器， 蛋白质和/或肽。 上述方法包括使用通过将含阳离子基团的聚合物与蛋白质和/或肽偶联或结合作为引入靶蛋白和/或肽的载体产生的缀合物。
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公开(公告)号：US07824910B2
公开(公告)日：2010-11-02
申请号：US10306165
申请日：2002-11-29
IPC分类号： C12N5/00
CPC分类号： A61K47/59
摘要： The invention relates to a method for transducing a pharmaceutical protein or polypeptide that may be fluorescently labeled into a cell by forming a conjugate with a cationic polymer having cation values of more than 2 and no more than 250 and transducing the conjugate into the cell. The protein or peptide pharmaceutical agent is directly covalently bound to the cationic polymer or is covalently bound to the cationic polymer through a bifunctional crosslinking agent.
摘要翻译： 本发明涉及通过与阳离子值大于2且不超过250并且将缀合物转导入细胞的阳离子聚合物形成缀合物来转导可以被荧光标记到细胞中的药物蛋白质或多肽的方法。 蛋白质或肽药剂直接共价结合阳离子聚合物，或通过双功能交联剂共价结合阳离子聚合物。
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公开(公告)号：US20120040390A1
公开(公告)日：2012-02-16
申请号：US11597675
申请日：2005-05-20
CPC分类号： G01N33/5005 , C12N5/0006 , G01N33/68
摘要： The present invention provides a method of intracellular introduction by which a protein and/or peptide can be efficiently introduced into living cells and which makes it possible to effectively analyze the behavior and function of the protein and/or peptide introduced and can be suitably utilized/applied, for example in the technology “live cell imaging” which realizes the immunohistochemical visualization in living cells under physiological conditions.The method of intracellular introduction is a method for introducing a protein and/or peptide into a cell, which comprises the process of introducing a conjugate generated by binding of a compound adsorbable on a cell surface to a protein and/or peptide via a polysulfide bond into a cell and treating with a reducing agent.
摘要翻译： 本发明提供了一种细胞内引入的方法，通过该方法可以将蛋白质和/或肽能够有效地引入活细胞中，并且这使得可以有效地分析引入的蛋白质和/或肽的行为和功能，并且可以适当地利用/ 应用于例如在生理条件下实现活细胞中的免疫组织化学可视化技术的“活细胞成像”。 细胞内引入的方法是将蛋白质和/或肽导入细胞的方法，其包括通过将可吸附在细胞表面上的化合物与蛋白质和/或肽经由多硫键结合产生的缀合物引入的方法 进入细胞并用还原剂处理。
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4.发明申请THERMOPLASTIC RESIN COMPOSITION, RESIN MOLDED ARTICLE AND POLARIZER PROTECTIVE FILM EACH USING THE SAME, AND METHOD OF PRODUCING THE RESIN MOLDED ARTICLE 审中-公开热塑性树脂组合物，树脂模制品和使用其的极性保护膜，以及生产树脂模制品的方法公开(公告)号：US20100182689A1
公开(公告)日：2010-07-22
申请号：US12664167
申请日：2008-06-13
申请人： Hidetaka Nakanishi , Akio Naka
发明人： Hidetaka Nakanishi , Akio Naka
IPC分类号： G02B1/08 , C08L33/08 , C08K5/3492 , C08L37/00 , C08L39/00
CPC分类号： C08G18/12 , C08G18/0823 , C08G18/4286 , C08G18/664 , C08K5/3492 , C09J175/12 , G02B1/04 , G02B1/105 , G02B1/14 , G02B5/208 , G02B5/3033 , C08L33/08 , C08G18/3246 , C08G18/706 , C08L33/064
摘要： Provided is a resin composition containing a thermoplastic acrylic resin and an ultraviolet absorber (UVA). While this resin composition has excellent heat resistance because of its high glass transition temperature, foaming and bleed-out can be suppressed and the problems arising from the evaporation of the UVA can be reduced even during the molding of the resin composition at a high temperature. The resin composition is a thermoplastic resin composition containing a thermoplastic acrylic resin and an ultraviolet absorber having a molecular weight of 700 or more, and having a glass transition temperature of 110° C. or higher. It is preferable that the ultraviolet absorber has a hydroxyphenyltriazine skeleton. It is preferable that the acrylic resin has a ring structure in its main chain. The ring structure is, for example, at least one selected from a lactone ring structure, a glutaric anhydride structure, a glutarimide structure, an N-substituted maleimide structure, and a maleic anhydride structure.
摘要翻译： 提供含有热塑性丙烯酸树脂和紫外线吸收剂（UVA）的树脂组合物。 虽然该树脂组合物由于其玻璃化转变温度高而具有优异的耐热性，但是可以抑制发泡和渗出，并且即使在高温下在树脂组合物的成型期间也可以降低由UVA的蒸发引起的问题。 树脂组合物是含有热塑性丙烯酸树脂和分子量为700以上，玻璃化转变温度为110℃以上的紫外线吸收剂的热塑性树脂组合物。 紫外线吸收剂优选具有羟基苯基三嗪骨架。 优选丙烯酸树脂在其主链中具有环结构。 环结构例如为选自内酯环结构，戊二酸酐结构，戊二酰亚胺结构，N-取代马来酰亚胺结构和马来酸酐结构中的至少一种。
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5.发明申请Lactone ring-containing polymer having few foreign matters and not easily causing gelation, and its applications 审中-公开内含物少，不容易引起凝胶化的内酯环聚合物及其应用公开(公告)号：US20070037962A1
公开(公告)日：2007-02-15
申请号：US11502355
申请日：2006-08-11
申请人： Ken-ichi Ueda , Shigeo Otome , Nobuhiro Maeda , Hidetaka Nakanishi , Hiroko Izumi , Hideaki Nagano
发明人： Ken-ichi Ueda , Shigeo Otome , Nobuhiro Maeda , Hidetaka Nakanishi , Hiroko Izumi , Hideaki Nagano
IPC分类号： C08G63/08
CPC分类号： C08F8/16 , C07C67/60 , C08F220/18 , C08F220/26 , C07C69/732
摘要： The lactone ring-containing polymer of the present invention meets the following conditions that: (1) the content of polymer gels of 50 μm or greater in average particle diameter is lower than or equal to 100 pieces/100 g, and the weight average molecular weight of the polymer is 50,000 to 170,000; and/or (2) an increasing rate of viscosity after heated at 280° C. for 30 minutes is 2.0 times or smaller. The lactone ring-containing polymer of the present invention contains very few foreign matters and does not easily cause gelation, in addition to being excellent in transparency and heat resistance as well as having the desired properties such as mechanical strength and forming and processing properties. The forming material obtained by heating and granulating such a polymer are suitable for use in, for example, optical components such as light guide materials, optical lenses, and optical films.
摘要翻译： 本发明的含内酯环的聚合物满足以下条件：（1）平均粒径为50μm以上的聚合物凝胶的含量为100个/ 100g以下，重均分子量 聚合物的重量为50,000至170,000; 和/或（2）在280℃下加热30分钟后的粘度增加率为2.0倍以下。 本发明的含内酯环的聚合物除了具有优异的透明性和耐热性以及具有期望的性能如机械强度和成型加工性能外，还含有极少量的异物并且不容易引起凝胶化。 通过加热造粒这种聚合物而获得的成形材料适用于例如导光材料，光学透镜和光学膜等光学部件。
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公开(公告)号：US06730746B2
公开(公告)日：2004-05-04
申请号：US10113557
申请日：2002-04-02
IPC分类号： C08F22062
CPC分类号： B41M5/52 , B41M5/5218 , B41M5/5254 , C08F8/00 , C09D11/107 , C09D133/064 , C08F20/00 , C08L2666/54
摘要： The present invention has for its object to provide a curable resin composition having excellent film characteristics such as film-forming properties, drying properties at room temperature and excellent adhesion to various substrates and, as such, being applicable with advantage in a broad range of uses inclusive of paints, surface treating agents, such as primers, anchor-coating agents, etc., adhesives, pressure sensitive adhesives, printing inks, and ink binders and receptor layers for ink-jet printing. A curable resin composition comprising a carboxyl-containing polymer and a polyvalent metal compound, said carboxyl-containing polymer having a primary amino and/or secondary amino group.
摘要翻译： 本发明的目的是提供一种具有优异的膜特性如成膜性能，在室温下的干燥性能以及对各种基材的优异粘附性的固化性树脂组合物，并且因此可广泛应用于广泛的用途 包括油漆，表面处理剂，如底漆，锚涂剂等，粘合剂，压敏粘合剂，印刷油墨，油墨粘合剂和用于喷墨印刷的接受层。1.一种可固化树脂组合物，其包含含羧基的 聚合物和多价金属化合物，所述含羧基聚合物具有伯氨基和/或仲氨基。
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