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1.发明授权Test kit for intracellular introduction of protein and/or peptide and method of intracellular introduction 有权用于细胞内引入蛋白质和/或肽的测试试剂盒和细胞内引入方法公开(公告)号：US07252960B2
公开(公告)日：2007-08-07
申请号：US10671881
申请日：2003-09-29
摘要： A test kit for intracellular introduction of a protein and/or peptide and a method of such intracellular introduction, make it possible for a protein and/or peptide to be introduced into cells efficiently and in an expedient manner while preventing the function of the introduction target protein and/or peptide from being reduced. The above test kit comprises a container containing a cationic group-containing polymer and a container containing a reagent for coupling or binding the cationic group-containing polymer to the protein and/or peptide, or comprises a container containing a cationized conjugate to be coupled to the protein and/or peptide. The above method comprises using a conjugate generated by coupling or binding a cationic group-containing polymer to a protein and/or peptide as a carrier for the introduction target protein and/or peptide.
摘要翻译： 用于细胞内引入蛋白质和/或肽的测试试剂盒和这种细胞内引入的方法使得蛋白质和/或肽可以有效地和方便地引入细胞中，同时防止引入靶的功能 蛋白质和/或肽被还原。 上述测试试剂盒包括含有含阳离子基团的聚合物的容器和含有用于将含阳离子基团的聚合物与蛋白质和/或肽偶联或结合的试剂的容器，或包含含有阳离子化缀合物的容器， 蛋白质和/或肽。 上述方法包括使用通过将含阳离子基团的聚合物与蛋白质和/或肽偶联或结合作为引入靶蛋白和/或肽的载体产生的缀合物。
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公开(公告)号：US20120040390A1
公开(公告)日：2012-02-16
申请号：US11597675
申请日：2005-05-20
CPC分类号： G01N33/5005 , C12N5/0006 , G01N33/68
摘要： The present invention provides a method of intracellular introduction by which a protein and/or peptide can be efficiently introduced into living cells and which makes it possible to effectively analyze the behavior and function of the protein and/or peptide introduced and can be suitably utilized/applied, for example in the technology “live cell imaging” which realizes the immunohistochemical visualization in living cells under physiological conditions.The method of intracellular introduction is a method for introducing a protein and/or peptide into a cell, which comprises the process of introducing a conjugate generated by binding of a compound adsorbable on a cell surface to a protein and/or peptide via a polysulfide bond into a cell and treating with a reducing agent.
摘要翻译： 本发明提供了一种细胞内引入的方法，通过该方法可以将蛋白质和/或肽能够有效地引入活细胞中，并且这使得可以有效地分析引入的蛋白质和/或肽的行为和功能，并且可以适当地利用/ 应用于例如在生理条件下实现活细胞中的免疫组织化学可视化技术的“活细胞成像”。 细胞内引入的方法是将蛋白质和/或肽导入细胞的方法，其包括通过将可吸附在细胞表面上的化合物与蛋白质和/或肽经由多硫键结合产生的缀合物引入的方法 进入细胞并用还原剂处理。
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公开(公告)号：US07824910B2
公开(公告)日：2010-11-02
申请号：US10306165
申请日：2002-11-29
IPC分类号： C12N5/00
CPC分类号： A61K47/59
摘要： The invention relates to a method for transducing a pharmaceutical protein or polypeptide that may be fluorescently labeled into a cell by forming a conjugate with a cationic polymer having cation values of more than 2 and no more than 250 and transducing the conjugate into the cell. The protein or peptide pharmaceutical agent is directly covalently bound to the cationic polymer or is covalently bound to the cationic polymer through a bifunctional crosslinking agent.
摘要翻译： 本发明涉及通过与阳离子值大于2且不超过250并且将缀合物转导入细胞的阳离子聚合物形成缀合物来转导可以被荧光标记到细胞中的药物蛋白质或多肽的方法。 蛋白质或肽药剂直接共价结合阳离子聚合物，或通过双功能交联剂共价结合阳离子聚合物。
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4.发明申请THIOSULFONATE COMPOUND, REVERSIBLE CATIONIZATION AGENT FOR PROTEIN AND/OR PEPTIDE, AND METHOD FOR SOLUBILIZATION 有权蛋白质组合物，蛋白质和/或肽的可逆加速剂，以及溶解方法公开(公告)号：US20130096278A1
公开(公告)日：2013-04-18
申请号：US13636789
申请日：2011-03-24
申请人： Junichiro Futami , Hidenori Yamada , Go Kyuragi , Keiichiro Yagi
发明人： Junichiro Futami , Hidenori Yamada , Go Kyuragi , Keiichiro Yagi
IPC分类号： C07C381/04 , C07K2/00
CPC分类号： C07C381/04 , C07K1/145 , C07K2/00
摘要： The object of the present invention is to provide a novel thiosulfonate compound, a reversible cationization agent for protein and/or peptide, which can reversibly cationize a wider range of proteins and peptides with high stability of quality and accuracy and which are useful for a high degree of purification and recovery, as well as, a method for solubilization for protein and/or peptide using the agent.The present invention is a thiosulfonate compound having three or more cations derived from a quaternary ammonium group within one molecule.
摘要翻译： 本发明的目的是提供一种新型的硫代磺酸盐化合物，用于蛋白质和/或肽的可逆阳离子化剂，其可以以高质量和准确性的高稳定性可逆地阳离子化更宽范围的蛋白质和肽，并且其对于高 纯化和回收程度，以及使用该试剂溶解蛋白质和/或肽的方法。 本发明是在1分子内具有季铵基的3个以上阳离子的硫代磺酸盐化合物。
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5.发明授权Thiosulfonate compound, reversible cationization agent for protein and/or peptide, and method for solubilization 有权用于蛋白质和/或肽的硫代磺酸盐化合物，可逆阳离子化剂和溶解方法公开(公告)号：US08653240B2
公开(公告)日：2014-02-18
申请号：US13636789
申请日：2011-03-24
申请人： Junichiro Futami , Hidenori Yamada , Go Kyuragi , Keiichiro Yagi
发明人： Junichiro Futami , Hidenori Yamada , Go Kyuragi , Keiichiro Yagi
CPC分类号： C07C381/04 , C07K1/145 , C07K2/00
摘要： The object of the present invention is to provide a novel thiosulfonate compound, a reversible cationization agent for protein and/or peptide, which can reversibly cationize a wider range of proteins and peptides with high stability of quality and accuracy and which are useful for a high degree of purification and recovery, as well as, a method for solubilization for protein and/or peptide using the agent.The present invention is a thiosulfonate compound having three or more cations derived from a quaternary ammonium group within one molecule.
摘要翻译： 本发明的目的是提供一种新型的硫代磺酸盐化合物，用于蛋白质和/或肽的可逆阳离子化剂，其可以以高质量和准确性的高稳定性可逆地阳离子化更宽范围的蛋白质和肽，并且其对于高 纯化和回收程度，以及使用该试剂溶解蛋白质和/或肽的方法。 本发明是在1分子内具有季铵基的3个以上阳离子的硫代磺酸盐化合物。
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公开(公告)号：US10822384B2
公开(公告)日：2020-11-03
申请号：US14389016
申请日：2013-03-29
IPC分类号： C07K17/00 , G01N33/543 , C07K14/47 , G01N33/574 , G01N33/68 , C07K1/113
摘要： The present invention provides the following: a method for efficiently producing a reagent for detecting an antibody that specifically binds with an insoluble antigen protein present in a liquid sample; a reagent for antibody detection produced by the production method; and a use of the antibody. In a step for solubilizing an antigen protein, it is possible to efficiently solubilize and recover the antigen protein by using a cationizing agent; therefore, when compared to conventional methods, it is possible to efficiently produce a reagent for detecting an antibody that has bound to multiple antigen protein molecules in a carrier.
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7.发明申请公开(公告)号：US20150064801A1
公开(公告)日：2015-03-05
申请号：US14389016
申请日：2013-03-29
IPC分类号： C07K14/47 , G01N33/574
CPC分类号： C07K14/4748 , C07K1/113 , C07K14/4703 , G01N33/543 , G01N33/574 , G01N33/57426 , G01N33/57492 , G01N33/6854 , G01N2333/47 , G01N2333/4704
摘要： The present invention provides the following: a method for efficiently producing a reagent for detecting an antibody that specifically binds with an insoluble antigen protein present in a liquid sample; a reagent for antibody detection produced by the production method; and a use of the antibody. In a step for solubilizing an antigen protein, it is possible to efficiently solubilize and recover the antigen protein by using a cationizing agent; therefore, when compared to conventional methods, it is possible to efficiently produce a reagent for detecting an antibody that has bound to multiple antigen protein molecules in a carrier.
摘要翻译： 本发明提供以下方法：一种用于有效地制备用于检测与存在于液体样品中的不溶性抗原蛋白特异性结合的抗体的试剂的方法; 通过该制造方法生产的抗体检测试剂; 和抗体的用途。 在溶解抗原蛋白的步骤中，可以通过使用阳离子化剂有效地溶解并回收抗原蛋白质; 因此，与常规方法相比，可以有效地制备用于检测在载体中与多个抗原蛋白质分子结合的抗体的试剂。
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公开(公告)号：US20130267025A1
公开(公告)日：2013-10-10
申请号：US13807747
申请日：2011-07-01
申请人： Hiromi Kumon , Masami Watanabe , Junichiro Futami , Yasuyuki Fujii , Hideo Ueki , Kazuhiko Ochiai
发明人： Hiromi Kumon , Masami Watanabe , Junichiro Futami , Yasuyuki Fujii , Hideo Ueki , Kazuhiko Ochiai
IPC分类号： C07K14/47
CPC分类号： C07K14/4747 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4703
摘要： A polypeptide capable of strongly inducing and activating dendritic-cell-like cells for treating or prevent cancer by immunotherapy, and DNA encoding the polypeptide. The polypeptide is a polypeptide (a) or (b) consisting of a partial region of the REIC/Dkk-3 protein.
摘要翻译： 能够强烈诱导和激活用于通过免疫治疗治疗或预防癌症的树突状细胞样细胞的多肽和编码该多肽的DNA。 多肽是由REIC / Dkk-3蛋白的部分区域组成的多肽（a）或（b）。
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