摘要:
A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q10 was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as Escherichia coli and expressing therein, coenzyme Q10 can be effectively produced.
摘要:
The present invention has for its object to isolate a gene coding for the enzyme synthesizing the coenzyme Q10 side chain from a fungal strain of the genus Saitoella and exploit it to advantage for the efficient microbial production of coenzyme Q10. The present invention provides; a DNA having the nucleotide sequence shown under SEQ ID NO:1 a DNA having a nucleotide sequence derived from the nucleotide sequence of SEQ ID NO:1 by the deletion, addition, insertion and/or substitution of one or a plurality of nucleotides and coding for a protein having decaprenyl diphosphate synthase activity a DNA which hybridizes with the DNA having the nucleotide sequence of SEQ ID NO:1 under a stringent condition and codes for a protein having decaprenyl diphosphate synthase activity.
摘要翻译:本发明的目的是从编码斋藤本的真菌菌株中分离编码合成辅酶Q10侧链的酶的基因,并有利于辅酶Q10的有效微生物产生。本发明提供了一种DNA 具有SEQ ID NO:1a所示的核苷酸序列,其具有通过删除,添加,插入和/或取代一个或多个核苷酸而编码具有SEQ ID NO:1的核苷酸序列的核苷酸序列的DNA,并编码具有SEQ ID NO: 癸酰二磷酸合酶活性a在严格条件下与具有SEQ ID NO:1的核苷酸序列的DNA杂交的DNA,并编码具有十聚丙二酰二磷酸合成酶活性的蛋白质的DNA。
摘要:
The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity.
摘要翻译:本发明的目的是通过利用源自属于曲霉属的真菌种的辅酶Q 10 N侧链合成基因提供在微生物中高效生产辅酶Q 10的方法,以及 白僵菌属。 本发明涉及具有SEQ ID NO:1和2所述的DNA序列或通过上述序列衍生的DNA序列的DNA,其通过一个或几个碱基的缺失,添加,插入和/或取代并编码具有十聚泼尼酯二磷酸合成酶活性的蛋白质 。
摘要:
The present disclosure describes a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present disclosure also describes a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a cell of a host organism.
摘要翻译:本公开描述了使用基因和蛋白质生产长链异戊烯基二磷酸合成酶(特别是十聚癸酸二磷酸合酶和solanesyl二磷酸合酶)的方法,所述基因和蛋白质是实现或增强真核生物长链的活性表达所必需的 异戊二烯基二磷酸合酶,以及使用微生物有效生产其侧链具有长链类异戊二烯(特别是辅酶Q9或辅酶Q10)的辅酶Q的方法。 本公开还描述了具有SEQ ID NO:1,3或5所示碱基序列的DNA和通过其一至几个碱基的缺失,添加,插入和/或取代从上述碱基序列衍生的DNA序列,以及 编码能够(功能)或增强真核生物衍生的长链异戊烯基二磷酸合成酶在宿主生物细胞中的活性表达的蛋白质。
摘要:
The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present invention relates to a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a host microorganism.
摘要翻译:本发明提供使用基因和蛋白质生产长链异戊烯基二磷酸合成酶(特别是十聚癸酸二磷酸合成酶和茄酰基二磷酸合成酶)的方法,所述基因和蛋白质是实现或增强真核生物长链的活性表达所必需的 异戊二烯基二磷酸合酶以及在其侧链(特别是辅酶Q 9或辅酶Q 10 N)中有效生产具有长链类异戊二烯的辅酶Q的方法,其使用 微生物。 本发明涉及具有SEQ ID NO:1,3或5所示碱基序列的DNA和通过其一至几个碱基的缺失,添加,插入和/或取代而衍生自上述碱基序列的DNA序列,以及 编码能够(功能)或增强宿主微生物中真核生物衍生的长链异戊二烯基二磷酸合成酶的活性表达的蛋白质。
摘要:
The present invention relates to production of ubiquinone-10 in E. coli by isolating and sequencing a structural gene dds1 of decaprenyl diphosphate synthase derived from photosynthetic bacteria such as Rhodobacter capsulatus., and expressing it in E. coli.
摘要:
A job execution system comprises a job execution apparatus and a server apparatus interconnected via a network. The server apparatus stores in itself, counter data defining the range of using the job execution apparatus, which is allowed for a user who logs in the job execution apparatus by authentication. The job execution apparatus obtains entirely or partially the counter data of the login user from the server apparatus; returns the counter data that is reduced by job execution to the server apparatus when the user logs out thereof; judges according to conditions set on the job execution apparatus itself, whether or not the counter data returning operation should be suspended; and suspends the counter data returning operation if judges that it should be suspended.
摘要:
The present invention provides a computer-readable recording medium having recorded therein an address book transmission program for causing a computer, which includes a storage that stores therein one or more pieces of address book data, to transmit the pieces of address book data to a data transmission apparatus, each of the pieces of address book data corresponding to one or more senders, the address book transmission program causing the computer to execute: a receiving step of receiving, from the data transmission apparatus, apparatus information indicating a state of the data transmission apparatus; a reading step of reading a piece of address book data that corresponds to a sender out of the pieces of address book data stored in the storage; a judging step of, based on the apparatus information received in the receiving step, specifying a use mode that shows how the data transmission apparatus uses address book data, and judging whether or not the piece of address book data read in the reading step is appropriate to the specified use mode; an editing step of, if a result of the judging step is negative, editing the read piece of address book data so as to be appropriate to the specified use mode; and a transmitting step of transmitting the edited piece of address book data to the data transmission apparatus.
摘要:
A subsystem of a first kind includes a first fee table receiving portion for receiving a first fee table, a user-restricted provision portion for providing a service, and an uncollected history record transmitting portion to transmit to a summation server an uncollected history record including a history record of providing a service. A subsystem of a second kind includes a post-collection provision portion for providing a service if a fee defined by a second fee table is collected, a collected history record transmitting portion to transmit to the summation server a collected history record including a history record of providing a service, a proxy provision portion for providing a service before a fee for the service is collected, and a switch control portion for comparing the first fee table with the second fee table and allowing the post-collection provision portion or the proxy provision portion to provide a service.
摘要:
An image processing system includes an information processing apparatus and an image processing apparatus connected to each other via a network. The information processing apparatus has an application installed thereon to give a new function to the image processing apparatus. The image processing apparatus transmits to the information processing apparatus, voice data obtained by a microphone of the image processing apparatus and data set via an operation screen customized according to the application. The information processing apparatus determines answer information indicating an action to be taken by the image processing apparatus, based on the received voice data, a dictionary owned by the application and the data set via the operation screen, and then transmits the determined answer information to the image processing apparatus. The image processing apparatus takes an action according to the answer information received therefrom.