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公开(公告)号：US20120231490A1
公开(公告)日：2012-09-13
申请号：US13504149
申请日：2010-10-22
IPC分类号： C12N15/861 , C12Q1/02 , C12N5/10
CPC分类号： C12N5/067 , C12N2500/25 , C12N2500/36 , C12N2500/44 , C12N2500/46 , C12N2501/115 , C12N2501/119 , C12N2501/155 , C12N2501/16 , C12N2501/60 , C12N2501/91 , C12N2506/02 , C12N2506/45 , C12N2510/00
摘要： Disclosed are: a gene transduction method for use in the induction of the differentiation of stem cells such as ES cells or iPS cells into hepatocytes effectively; stem cells into each of which a gene useful for the induction of the differentiation into hepatocytes is introduced; and hepatocytes produced from stem cells each having the gene introduced therein. A specific gene can be introduced into stem cells such as ES cells or iPS cells using an adenovirus vector. The effective induction of the differentiation into hepatocytes can be achieved by introducing the gene. Specifically, the effective induction of the differentiation of stem cells such as ES cells or iPS cells into hepatocytes can be achieved by introducing at least one gene selected from HEX gene, HNF4A gene, HNF6 gene and SOX17 gene into the stem cells.
摘要翻译： 公开的是：用于诱导干细胞如ES细胞或iPS细胞分化成肝细胞的基因转导方法; 引入干细胞，其中每一种可用于诱导分化成肝细胞的基因; 和从其中引入基因的干细胞产生的肝细胞。 可以使用腺病毒载体将特定基因导入干细胞如ES细胞或iPS细胞。 可以通过引入基因来实现分化成肝细胞的有效诱导。 具体地说，通过将至少一种选自HEX基因，HNF4A基因，HNF6基因和SOX17基因的基因导入干细胞，可以有效诱导干细胞如ES细胞或iPS细胞分化成肝细胞。
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公开(公告)号：US20130309768A1
公开(公告)日：2013-11-21
申请号：US13982127
申请日：2011-08-24
申请人： Miho Furue , Masaki Kinehara
发明人： Miho Furue , Masaki Kinehara
IPC分类号： C12N5/0735
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2500/25 , C12N2500/44 , C12N2500/46 , C12N2501/998
摘要： An aim of the present invention is to provide a method for culturing a human pluripotent stem cell while maintaining an undifferentiated state, more efficiently than conventional methods, and a kit therefor. The pluripotency of a stem cell was found to be maintained at a high rate, regardless of experimenter's proficiency in culturing techniques, by (a) culturing a human pluripotent stem cell in a first medium which a medium for pluripotent stem cell comprising activin; (b) replacing the first medium with a second medium which is a medium for pluripotent stem cell comprising no activin, and culturing the human pluripotent stem cell, (c) subculturing the human pluripotent stem cell into the first medium, and then repeating the above (b) and (c) sequentially.
摘要翻译： 本发明的目的是提供一种培养人多能干细胞同时保持未分化状态，比常规方法更有效的方法及其用途。 通过（a）在第一培养基中培养人多能干细胞，发现干细胞的多能性以高速率维持，而不管实验者对培养技术的熟练程度，培养人多能干细胞是一种包含激活素的多能干细胞培养基; （b）用第二培养基替换第一培养基，第二培养基是不含激活素的多能干细胞的培养基，培养人多能干细胞，（c）将人多能干细胞传代培养到第一培养基中，然后重复上述 （b）和（c）。
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公开(公告)号：US20080050817A1
公开(公告)日：2008-02-28
申请号：US10584371
申请日：2004-12-27
申请人： Miho Furue , Tetsuji Okamoto , Makoto Asashima
发明人： Miho Furue , Tetsuji Okamoto , Makoto Asashima
IPC分类号： C12N5/02
CPC分类号： C12N5/0606 , C12N5/0037 , C12N2500/90
摘要： The present invention discloses a medium for a serum-free medium capable of culturing ES cells for a long period while maintaining their undifferentiated state without using feeder cells, and a basal medium for producing the medium thus described. The basal medium of the present invention is characterized by that it has composition shown by Table I. Further, the present invention discloses a medium for ES cells produced with the basal medium.
摘要翻译： 本发明公开了一种能够长时间培养ES细胞，同时保持其未分化状态而不使用饲养细胞的无血清培养基，以及用于生产如此描述的培养基的基础培养基。 本发明的基础培养基的特征在于其具有表I所示的组成。此外，本发明公开了一种用基础培养基生产的ES细胞培养基。
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公开(公告)号：US07923245B2
公开(公告)日：2011-04-12
申请号：US10584371
申请日：2004-12-27
申请人： Miho Furue , Tetsuji Okamoto , Makoto Asashima
发明人： Miho Furue , Tetsuji Okamoto , Makoto Asashima
CPC分类号： C12N5/0606 , C12N5/0037 , C12N2500/90
摘要： The present invention discloses a medium for a serum-free medium capable of culturing ES cells for a long period while maintaining their undifferentiated state without using feeder cells, and a basal medium for producing the medium thus described. The basal medium of the present invention is characterized by that it has composition shown by Table I. Further, the present invention discloses a medium for ES cells produced with the basal medium.
摘要翻译： 本发明公开了一种能够长时间培养ES细胞，同时保持其未分化状态而不使用饲养细胞的无血清培养基，以及用于生产如此描述的培养基的基础培养基。 本发明的基础培养基的特征在于其具有表I所示的组成。此外，本发明公开了一种用基础培养基生产的ES细胞培养基。
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