公开(公告)号：US5706498A

公开(公告)日：1998-01-06

申请号：US312689

申请日：1994-09-26

申请人： Hitoshi Fujimiya ,  Shinichiro Yamamoto ,  Norio Maru ,  Toshitsugu Okayama ,  Hisanori Nasu

发明人： Hitoshi Fujimiya ,  Shinichiro Yamamoto ,  Norio Maru ,  Toshitsugu Okayama ,  Hisanori Nasu

IPC分类号： C12N15/09 ,  G06F17/30 ,  G06F19/22 ,  G06F19/28 ,  G06F13/12

CPC分类号： G06F19/28 ,  Y10S707/99936

摘要： The gene database retrieval system, for making a retrieval for a gene sequence having a sequence similar to a sequence data from a gene database, contains a gene database for storing the sequence data of genes whose structures or sequences have already been analyzed and identified, a dynamic programming operation unit for determining the degree of similarity between target data and key data by utilizing the sequence data of the bases of the gene from the gene database as the target data and the sequence data of the bases as the key for retrieval, and a central processing device unit for executing the access process to make access to the gene database, in parallel to the operation process for determining the degree of similarity by transmitting the sequence data of the bases from the gene database continually one after another into the dynamic programming operation unit as the target data, by controlling the gene database and the dynamic programming operation unit.

摘要翻译： 用于对具有与基因数据库的序列数据类似的序列的基因序列的检索的基因数据库检索系统包含用于存储其结构或序列已被分析和鉴定的基因的序列数据的基因数据库， 用于通过利用来自基因数据库的基因的碱基的序列数据作为目标数据和碱基的序列数据作为检索关键来确定目标数据和关键数据之间的相似程度的动态规划操作单元，以及 中央处理装置单元，用于执行访问过程以访问基因数据库，与用于通过将基因的序列数据从基因数据库连续地依次发送到动态规划操作中来确定相似度的操作过程并行地进行 单元作为目标数据，通过控制基因数据库和动态编程操作单元。

公开(公告)号：US5246866A

公开(公告)日：1993-09-21

申请号：US849631

申请日：1992-03-02

申请人： Hisanori Nasu ,  Toshitsugu Okayama ,  Hitoshi Fujimiya

发明人： Hisanori Nasu ,  Toshitsugu Okayama ,  Hitoshi Fujimiya

IPC分类号： G01N27/447

CPC分类号： G01N27/44721 ,  Y10T436/143333

摘要： A DNA sequence transcribing method involves labeling a DNA fragment with a fluorescent substance, subjecting the labeled fragments to electrophoresis, exposing a gel containing the DNA fragment to light during electrophoresis, and detecting fluorescence generated upon exposure. An image corresponding to a fluorescent image on the gel is transcribed on a recording medium by entering a detecting signal in synchronization with operation of detecting fluorescence on the gel containing the DNA fragment after electrophoresis. This arrangement enables a crude fluorescent image on the gel containing the DNA fragment to be recorded as an intact image of the DNA sequencing on the basis of the fluorescent method, and the image can be retained.

摘要翻译： DNA序列转录方法包括用荧光物质标记DNA片段，对标记的片段进行电泳，在电泳期间将含有DNA片段的凝胶暴露于光，并检测在暴露时产生的荧光。 通过在电泳后含有DNA片段的凝胶上检测荧光的操作同步地输入检测信号，将与凝胶上的荧光图像对应的图像记录在记录介质上。 这种布置使得能够基于荧光方法将含有DNA片段的凝胶上的粗荧光图像记录为DNA测序的完整图像，并且可以保留图像。

公开(公告)号：US5190632A

公开(公告)日：1993-03-02

申请号：US854872

申请日：1992-03-20

申请人： Hitoshi Fujimiya ,  Hisanori Nasu

发明人： Hitoshi Fujimiya ,  Hisanori Nasu

IPC分类号： G01N21/64 ,  G01N27/447

CPC分类号： G01N27/44721

摘要： The multi-colored electrophoresis pattern reading system compares the patterns of electrophoresis without a warp to be caused upon electrophoresis by reading the patterns of electrophoresis which is carried out concurrently for plural samples and by labelling the samples with fluorescent substances having different fluorescent wavelengths. The patterns of electrophoresis are read by allowing fluorescence to emit from the plural samples. In reading the fluorescent patterns, optical signals are received by the light receiving section and then the fluorescence having a predetermined wavelength is separated by the fluorescent-wavelength separating section from the optical signals with the aid of an optical filter capable of controlling an angle of incidence relative to the optical axis of the optical filter. The optical signals passed through the fluorescent-wavelength separating section is then subjected to optoelectrical conversion into electric signals which in turn are subjected to signal processing, thereby converting the electric signals into a desired form of data representation.

公开(公告)号：US6077670A

公开(公告)日：2000-06-20

申请号：US39278

申请日：1998-03-12

申请人： Kenji Yamamoto ,  Hisanori Nasu ,  Hitoshi Fujimiya ,  Noriko Yurino

发明人： Kenji Yamamoto ,  Hisanori Nasu ,  Hitoshi Fujimiya ,  Noriko Yurino

IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/533 ,  G01N37/00

CPC分类号： C12Q1/6818

摘要： A method is disclosed which is capable of increasing a S/N ratio without subjecting a sample to any treatment to thereby detect hybridization with high sensitivity. A fragmentarily divided probe is used which is prepared by dividing a single probe 12 into a plurality of fragmentary probes 12a, 12b, and labeling the fragmentary probes with different fluorescent materials 14a, 14b, respectively. When the probe is hybridized with a sample 11, the fragmentary probes 12a,12b reconstitute the single probe. An intermittent light beam is used as excitation light 19. The fluorescent material 14a is excited therewith, and fluorescence 18 emitted from the fluorescent material 14b through multistep excitation is detected while irradiation with the excitation light is intermitted.

摘要翻译： 公开了能够提高S / N比而不对样品进行任何处理从而以高灵敏度检测杂交的方法。 使用通过将单个探针12分成多个部分探针12a，12b并分别用不同的荧光材料14a，14b标记碎片探针来制备的片断分离的探针。 当探针与样品11杂交时，片段探针12a，12b重构单个探针。 使用间歇光束作为激发光19.荧光材料14a被激发，并且通过多脉冲激发从荧光材料14b发射的荧光18被检测，同时激发光的照射被中断。

公开(公告)号：US5213673A

公开(公告)日：1993-05-25

申请号：US800847

申请日：1991-11-29

申请人： Hitoshi Fujimiya ,  Hisanori Nasu

发明人： Hitoshi Fujimiya ,  Hisanori Nasu

IPC分类号： G01N21/17 ,  G01N27/447

CPC分类号： G01N27/44721

摘要： A multi-colored electrophoresis pattern reading apparatus is capable of labelling each of plural samples separately with each of plural fluorescent substances having different fluorescence wavelengths, by electrophoresing the plural samples to develop a pattern, exciting the fluorescent substances labelled on the samples to emit fluorescence, and reading a fluorescent pattern emitting the fluorescence. The apparatus contains a source for the light for exciting the fluorescent substances; a light scanning mechanism for scanning the light to irradiate a gel in the direction of its thickness with the light; a light receiving section for receiving fluorescence resulting from the light source separated from the scattered light resulting from a reading surface on the basis of a spatial position relationship of a light receiving path by setting a light receiving surface in a direction different from an optical axis of the irradiating light; an optical filter section for separating the resulting optical signals section into plural wavelengths; an optoelectric conversion section for converting optical signals into electrical signals; and an amplifier for amplifying the electrical signals by an integrating operation corresponding to scanning of the irradiating light, and for generating electrical signals indicative of fluorescence from the pattern one after another.

公开(公告)号：US06576104B1

公开(公告)日：2003-06-10

申请号：US08212818

申请日：1994-03-15

申请人： Hisanori Nasu ,  Yoshitaka Nakamura ,  Hitoshi Fujimiya ,  Kenji Yamamoto

发明人： Hisanori Nasu ,  Yoshitaka Nakamura ,  Hitoshi Fujimiya ,  Kenji Yamamoto

IPC分类号： G01N2726

CPC分类号： G01N27/44721

摘要： An apparatus and a method for reading a gel electrophoresis pattern can read the gel electrophoresis pattern of a sample, such as a nucleic acid and a protein, with high sensitivity and without requiring the use of an expensive device structure such as a laser light source of unique type. The gel electrophoresis apparatus is structured by a sample supplying section for supplying a sample labeled with a fluorescent substance from an inlet side of an electrophoresis gel unit; an electrophoresis unit for subjecting the sample to electrophoresis by applying an electrophoresis voltage to the electrophoresis gel unit; a carrying section for withdrawing the electrophoresed sample continually from an outlet side of the electrophoresis gel unit into a carrying fluid and carrying the fluid a predetermined distance; a mixing unit for mixing the carrying fluid with a luminous liquid; and a light receiving section for receiving fluorescence emitted from the sample in the luminous liquid at a position to which the carrying fluid has been carried the predetermined distance.

摘要翻译： 读取凝胶电泳图案的装置和方法可以高灵敏度地读取样品（例如核酸和蛋白质）的凝胶电泳图案，并且不需要使用昂贵的器件结构，例如激光光源 独特的类型。 凝胶电泳装置由用于从电泳凝胶单元的入口侧供给标记有荧光物质的样品的样品供给部构成， 电泳单元，其通过向电泳凝胶单元施加电泳电压使样品进行电泳; 用于将电泳样品从电泳凝胶单元的出口侧连续地抽出到携带流体中并将流体携带预定距离的承载部分; 用于将运载流体与发光液体混合的混合单元; 以及受光部，用于在所述承载流体已经承载了所述预定距离的位置处接收从所述发光液体中的所述样品发射的荧光。

公开(公告)号：US5991030A

公开(公告)日：1999-11-23

申请号：US66875

申请日：1998-04-28

申请人： Kenji Yamamoto ,  Hisanori Nasu ,  Toshimasa Watanabe ,  Yuuji Tsukamoto ,  Tateo Kondou ,  Takehiko Nishida ,  Hitoshi Fujimiya ,  Noriko Yurino

发明人： Kenji Yamamoto ,  Hisanori Nasu ,  Toshimasa Watanabe ,  Yuuji Tsukamoto ,  Tateo Kondou ,  Takehiko Nishida ,  Hitoshi Fujimiya ,  Noriko Yurino

IPC分类号： G01N27/447 ,  G01R9/02 ,  F21V9/16

CPC分类号： G01N27/44721

摘要： The apparatus for reading a luminescence pattern is equipped with a stage on which the sample to be read is mounted. A photoreceptor disk is installed in the lower part of the stage and scans and condenses light from the luminescent pattern of the sample at positions determined by the rotation of a rotary plate. A transport mechanism causes the stage and the photoreceptor disk to undergo relative motion. An optical guide part guides the light from the luminescent pattern to a photoelectric converter. The optical guide part may include mirrors or one or more optical fibers. The photoelectric converter converts the light into an electrical signal. A controller controls how scanning is performed by the photoreceptor disk and transport mechanism and generates a read scanning position signal. A data processor performs data processing by converting the electrical signal into a digital signal and obtains a scanning position signal from the controller. Additionally, a laser light source may be used to shine laser light onto an electrophoresis gel of the sample.

摘要翻译： 用于读取发光图案的装置装备有待安装样品的阶段。 感光盘安装在舞台的下部，并且通过旋转板旋转确定的位置扫描并冷凝来自样品的发光图案的光。 传送机构使舞台和感光盘发生相对运动。 光导部分将光从发光图案引导到光电转换器。 光导部分可以包括反射镜或一个或多个光纤。 光电转换器将光转换成电信号。 控制器控制感光盘和传送机构的扫描方式，并生成读取扫描位置信号。 数据处理器通过将电信号转换为数字信号并从控制器获得扫描位置信号来执行数据处理。 此外，可以使用激光源将激光照射到样品的电泳凝胶上。

公开(公告)号：USRE36826E

公开(公告)日：2000-08-22

申请号：US160747

申请日：1993-12-03

申请人： Hitoshi Fujimiya ,  Shigeo Nakajima ,  Hisanori Nasu ,  Keigi Koga

发明人： Hitoshi Fujimiya ,  Shigeo Nakajima ,  Hisanori Nasu ,  Keigi Koga

IPC分类号： G01N21/64 ,  G01N21/17 ,  G01N27/447 ,  C25B7/00 ,  C25B9/00

CPC分类号： G01N27/44704 ,  G01N27/44721

摘要： An electrophoresis pattern reading system of fluorescent type is comprised of a detachable migration unit comprising a gel functioning as a base for electrophoresis and a gel-supporting body for supporting the gel; an electrophoresis unit, to which the migration unit is mounted, for performing electrophoresis by applying migrating voltage to the gel to which a sample labeled with a fluorescent substance is added; and a reading unit including an instrumentation subunit for reading an electrophoresis pattern, to which the migration unit is mounted after electrophoresis and which receives fluorescence emitted from the fluorescent substance of the sample on the gel upon application of light to the gel. For example, a plurality of the migration units and the electrophoresis units are provided, and each of the plural migration units are mounted to one reading unit in order after electrophoresis has been performed with the respective electrophoresis units for a long period of time, thereby reading the electrophoresis pattern in a short period of time.

摘要翻译： 荧光型电泳图案读取系统由包含用作电泳基底的凝胶和用于支撑凝胶的凝胶支撑体构成的可拆卸迁移单元构成; 电泳单元，安装有迁移单元的电泳单元，用于通过向添加有荧光物质标记的样品的凝胶施加迁移电压来进行电泳; 以及读取单元，其包括用于读取电泳图案的仪器子单元，电泳后安装迁移单元，并且在向凝胶施加光时接收从凝胶上的样品的荧光物质发出的荧光。 例如，设置多个迁移单元和电泳单元，并且在各电泳单元长时间进行电泳以后，将多个迁移单元中的每一个依次安装到一个读取单元，从而读取 电泳模式在短时间内。

公开(公告)号：US5953133A

公开(公告)日：1999-09-14

申请号：US641424

申请日：1996-05-01

申请人： Hitoshi Fujimiya ,  Kenji Yamamoto ,  Toshiaki Ito ,  Hisanori Nasu

发明人： Hitoshi Fujimiya ,  Kenji Yamamoto ,  Toshiaki Ito ,  Hisanori Nasu

IPC分类号： G01N27/447 ,  G02F1/055 ,  G06T1/00 ,  H04N1/04 ,  G01B11/00 ,  G01N21/00 ,  H01L31/14

CPC分类号： G01N27/44721

摘要： A scanning apparatus for scanning and reading a luminescent pattern of a sample in a flat-plate shape, having a placing member for placing the sample as an object for reading; a light condensing member for condensing light emitted from the luminescent pattern of the sample; a movement member for moving the light condensing member relative to the placing member; a light receiving member for dividing the light of the luminescent pattern of the sample condensed by the light condensing member into predetermined segments and receiving the light by scanning the light from the segments in a one-dimensional way; a photoelectrical conversion member for converting optical signals of the light received by the light receiving member into electrical signals; a control member for controlling a scan by the light receiving member in accordance with the electrical signals from the photoelectrical conversion member; and a data processing member for converting the electrical signals from the photoelectrical conversion member into digital signals and reconfiguring an image from the optical signals of the light from the segment received selectively in a one-dimensional way. The scanning apparatus can likewise scan and read a non-luminous or less luminous pattern of a sample by allowing light from a plane light source to transmit through the pattern of the sample.

摘要翻译： 一种用于扫描和读取平板形状的样品的发光图案的扫描装置，具有用于放置样品作为读取对象的放置构件; 用于聚集从样品的发光图案发出的光的聚光部件; 用于使所述聚光部件相对于所述放置部件移动的移动部件; 光接收部件，用于将通过聚光部件冷凝的样品的发光图案的光分成预定的段，并以一维方式扫描来自该段的光; 光电转换构件，用于将由光接收构件接收的光的光信号转换为电信号; 控制构件，用于根据来自光电转换构件的电信号控制受光构件的扫描; 以及数据处理部件，用于将来自光电转换部件的电信号转换成数字信号，并且以一维方式从选择性地接收来自光盘的光的光信号重构图像。 扫描装置同样可以通过允许来自平面光源的光透过样品的图案来扫描和读取样品的非发光或更少的发光图案。

公开(公告)号：US5616228A

公开(公告)日：1997-04-01

申请号：US535955

申请日：1995-09-28

申请人： Hisanori Nasu ,  Kenji Yamamoto ,  Hitoshi Fujimiya

发明人： Hisanori Nasu ,  Kenji Yamamoto ,  Hitoshi Fujimiya

IPC分类号： G01N21/64 ,  G01N27/447 ,  G01N33/50 ,  G01N27/26

CPC分类号： G01N27/44721

摘要： The electrophoresis apparatus has a capillary filled with gel for use in electrophoresis. A first buffer solution container is provided for storing a buffer solution and introducing a sample labelled with a fluorescent substance into an inlet of said capillary for electrophoresis. A second buffer solution container is provided for storing a buffer solution containing a luminous solution, into which said sample is continually introduced from an outlet of said capillary after electrophoresis. Electrophoresis means is provided for subjecting said sample to electrophoresis by applying a predetermined value of voltage to said gel through which said sample is being transferred while being electrophoresed. Light receiving means is disposed underneath the outlet of said capillary to read fluorescence emitted from said sample from bottom in a direction in which said sample is approaching closer. This electrophoresis apparatus has benefits in that it can read an electrophoresis pattern of a sample of a nucleic acid or a protein obtained by subjecting the sample to electrophoresis with a high sensitivity without requiring any expensive device structure such as a laser beam source system. Thus, it is cheaper than conventional systems requiring such a laser beam system and is easier to handle.

摘要翻译： 电泳装置具有填充有用于电泳的凝胶的毛细管。 提供第一缓冲溶液容器用于储存缓冲溶液，并将标记有荧光物质的样品引入所述毛细管的入口进行电泳。 提供第二缓冲溶液容器，用于储存含有发光溶液的缓冲溶液，所述样品在电泳后从所述毛细管的出口连续地引入其中。 提供电泳装置，用于对所述样品进行电泳，通过在电泳时向所述样品转移所述凝胶施加预定值的电压。 光接收装置设置在所述毛细管的出口下方，以从所述样品靠近的方向从底部读取从所述样品发出的荧光。 该电泳装置的优点在于，可以读取通过使样品以高灵敏度进行电泳而获得的核酸或蛋白样品的电泳图案，而不需要任何昂贵的器件结构，例如激光束源系统。 因此，它比需要这种激光束系统的传统系统便宜，并且更容易处理。