公开(公告)号：US07384792B1

公开(公告)日：2008-06-10

申请号：US10852787

申请日：2004-05-24

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: G01N21/03 ,  G01N33/558 ,  B32B3/02 ,  B32B7/12 ,  H01J9/04

CPC classification number: G01N21/658 ,  G01J3/44 ,  Y10T428/218 ,  Y10T428/28

Abstract: A method to fabricate an optical scattering probe and the method includes the steps of a) depositing an conductive layer on a substrate followed by depositing a noble metal layer on top of the conductive layer and then an aluminum layer on top the noble metal layer; b) anodizing the aluminum layer to form a porous aluminum oxide layer having a plurality of pores; and c) etching the plurality of pores through the aluminum oxide layer and the noble metal layer for forming a nano-hole array. In a preferred embodiment, the step of etching the plurality of pores through the aluminum oxide layer and the noble metal layer further comprising a step of widening the pores followed by removing the aluminum oxide layer for forming a plurality of noble metal column on top of the conductive layer.

Abstract translation: 一种制造光散射探针的方法，包括以下步骤：a）在衬底上沉积导电层，然后在导电层的顶部沉积贵金属层，然后在贵金属层顶上沉积铝层; b）阳极氧化铝层以形成具有多个孔的多孔氧化铝层; 以及c）通过氧化铝层和用于形成纳米孔阵列的贵金属层蚀刻多个孔。 在优选实施例中，通过氧化铝层和贵金属层蚀刻多个孔的步骤还包括扩大孔的步骤，随后除去用于形成多个贵金属柱的氧化铝层 导电层。

公开(公告)号：US07892489B2

公开(公告)日：2011-02-22

申请号：US11754912

申请日：2007-05-29

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: G01N21/00 ,  G01N21/03 ,  G01J3/44

CPC classification number: G01N21/658

Abstract: A micro structure includes a silicon substrate, an adhesion layer on the silicon substrate, a bias layer on the adhesion layer, and structure layers on the adhesion layer. The two or more structure layers comprise different material compositions and a plurality of holes through at least two of the structure layers. Widths of the plurality of holes are in the range of 0.5-500 nm.

Abstract translation: 微结构包括硅衬底，硅衬底上的粘合层，粘合层上的偏置层和粘合层上的结构层。 两个或更多个结构层包括不同的材料组成和通过至少两个结构层的多个孔。 多个孔的宽度在0.5〜500nm的范围内。

公开(公告)号：US07242469B2

公开(公告)日：2007-07-10

申请号：US10987842

申请日：2004-11-12

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: G01J3/44

CPC classification number: G01J3/44 ,  G01N21/658 ,  G08B21/12

Abstract: New and improved applications of Raman Scattering are disclosed. These applications may be implemented with or without using an enhanced nano-structured surface that is trademarked as the RamanNanoChip™ disclosed in a pending patent. As a RamanNanoChip™ provides much higher sensitivity in SERS compared with conventional enhance surface, broader scopes of applications are now enabled and can be practically implemented as now disclosed in this application. Furthermore, a wide range of applications is achievable as new and improved Raman sensing applications. By applying the analysis of Raman scattering spectrum, applications can be carried out to identify unknown chemical compositions to perform the tasks of homeland security; food, drug and drinking materials safety; early disease diagnosis; environmental monitoring; industrial process monitoring, precious metal and gem authentications, etc.

Abstract translation: 公开了拉曼散射的新的和改进的应用。 这些应用可以在使用或不使用商标为未决专利中公开的RamanNanoChip TM的增强型纳米结构化表面的情况下实现。 由于与传统增强表面相比，RamanNanoChip（TM）在SERS中提供了更高的灵敏度，因此现在可以实现更广泛的应用范围，并且可以实际实现，如本应用中所公开的。 此外，作为新的和改进的拉曼传感应用可以实现广泛的应用。 通过应用拉曼散射光谱分析，可以应用于识别未知化学成分来执行国土安全任务; 食品，药品和饮料安全; 早期疾病诊断; 环境监测; 工业过程监控，贵金属和宝石认证等

公开(公告)号：US20050206892A1

公开(公告)日：2005-09-22

申请号：US10987842

申请日：2004-11-12

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01J3/44 ,  G01N21/658 ,  G08B21/12

Abstract: New and improved applications of Raman Scattering are disclosed. These applications may be implemented with or without using an enhanced nano-structured surface that is trademarked as the RamanNanoChip™ disclosed in a pending patent. As a RamanNanoChip™ provides much higher sensitivity in SERS compared with conventional enhance surface, broader scopes of applications are now enabled and can be practically implemented as now disclosed in this application. Furthermore, a wide range of applications is achievable as new and improved Raman sensing applications. By applying the analysis of Raman scattering spectrum, applications can be carried out to identify unknown chemical compositions to perform the tasks of homeland security; food, drug and drinking materials safety; early disease diagnosis; environmental monitoring; industrial process monitoring, precious metal and gem authentications, etc.

Abstract translation: 公开了拉曼散射的新的和改进的应用。 这些应用可以在使用或不使用商标为未决专利中公开的RamanNanoChip TM的增强型纳米结构化表面的情况下实现。 由于与传统增强表面相比，RamanNanoChip（TM）在SERS中提供了更高的灵敏度，因此现在可以实现更广泛的应用范围，并且可以实际实现，如本应用中所公开的。 此外，作为新的和改进的拉曼传感应用可以实现广泛的应用。 通过应用拉曼散射光谱分析，可以应用于识别未知化学成分来执行国土安全任务; 食品，药品和饮料安全; 早期疾病诊断; 环境监测; 工业过程监控，贵金属和宝石认证等

公开(公告)号：US08323580B2

公开(公告)日：2012-12-04

申请号：US13030274

申请日：2011-02-18

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: B01J19/08 ,  G01N33/553 ,  G01J3/44

CPC classification number: G01N21/658

Abstract: A micro structure for sensing a substance using light scattering includes a substrate, a first layer on the substrate, wherein the first layer comprises a metallic material, a second layer over the first layer, and a mask layer over the second layer. A plurality of nano holes are formed through the mask layer and the second layer, wherein the plurality of holes are defined in part by internal surfaces on the second layer and the mask layer. Two or more structure layers are formed on the mask layer and the internal surfaces in the plurality of holes. The two or more structure layers comprise different material compositions.

Abstract translation: 用于使用光散射感测物质的微结构包括基底，基底上的第一层，其中第一层包括金属材料，第一层上的第二层和第二层上的掩模层。 通过掩模层和第二层形成多个纳米孔，其中多个孔部分地由第二层上的内表面和掩模层限定。 在掩模层和多个孔中的内表面上形成两层以上的结构层。 两个或多个结构层包括不同的材料组成。

公开(公告)号：US20070153269A1

公开(公告)日：2007-07-05

申请号：US11681157

申请日：2007-03-01

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01J3/44 ,  G01N21/658 ,  G08B21/12

Abstract: New and improved applications of Raman Scattering are disclosed. These applications may be implemented with or without using an enhanced nano-structured surface that is trademarked as the RamanNanoChip™ disclosed in a pending patent. As a RamanNanoChip™ provides much higher sensitivity in SERS compared with conventional enhance surface, broader scopes of applications are now enabled and can be practically implemented as now disclosed in this application. Furthermore, a wide range of applications is achievable as new and improved Raman sensing applications. By applying the analysis of Raman scattering spectrum, applications can be carried out to identify unknown chemical compositions to perform the tasks of homeland security; food, drug and drinking materials safety; early disease diagnosis environmental monitoring; industrial process monitoring, precious metal and gem authentications, etc.

Abstract translation: 公开了拉曼散射的新的和改进的应用。 这些应用可以在使用或不使用商标为未决专利中公开的RamanNanoChip TM的增强型纳米结构化表面的情况下实现。 由于与传统增强表面相比，RamanNanoChip（TM）在SERS中提供了更高的灵敏度，因此现在可以实现更广泛的应用范围，并且可以实际实现，如本应用中所公开的。 此外，作为新的和改进的拉曼传感应用可以实现广泛的应用。 通过应用拉曼散射光谱分析，可以应用于识别未知化学成分来执行国土安全任务; 食品，药品和饮料安全; 早期疾病诊断环境监测; 工业过程监控，贵金属和宝石认证等

公开(公告)号：US20110140209A1

公开(公告)日：2011-06-16

申请号：US13030274

申请日：2011-02-18

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: H01L29/66 ,  H01L21/302

CPC classification number: G01N21/658

Abstract: A micro structure for sensing a substance using light scattering includes a substrate, a first layer on the substrate, wherein the first layer comprises a metallic material, a second layer over the first layer, and a mask layer over the second layer. A plurality of nano holes are formed through the mask layer and the second layer, wherein the plurality of holes are defined in part by internal surfaces on the second layer and the mask layer. Two or more structure layers are formed on the mask layer and the internal surfaces in the plurality of holes. The two or more structure layers comprise different material compositions.

Abstract translation: 用于使用光散射感测物质的微结构包括基底，基底上的第一层，其中第一层包括金属材料，第一层上的第二层和第二层上的掩模层。 通过掩模层和第二层形成多个纳米孔，其中多个孔部分地由第二层上的内表面和掩模层限定。 在掩模层和多个孔中的内表面上形成两层以上的结构层。 两个或多个结构层包括不同的材料组成。

公开(公告)号：US07428046B2

公开(公告)日：2008-09-23

申请号：US11681157

申请日：2007-03-01

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: G01J3/44

CPC classification number: G01J3/44 ,  G01N21/658 ,  G08B21/12

Abstract: New and improved applications of Raman Scattering are disclosed. These applications may be implemented with or without using an enhanced nano-structured surface that is trademarked as the RamanNanoChip™ disclosed in a pending patent. As a RamanNanoChip™ provides much higher sensitivity in SERS compared with conventional enhance surface, broader scopes of applications are now enabled and can be practically implemented as now disclosed in this application. Furthermore, a wide range of applications is achievable as new and improved Raman sensing applications. By applying the analysis of Raman scattering spectrum, applications can be carried out to identify unknown chemical compositions to perform the tasks of homeland security; food, drug and drinking materials safety; early disease diagnosis environmental monitoring; industrial process monitoring, precious metal and gem authentications, etc.

Abstract translation: 公开了拉曼散射的新的和改进的应用。 这些应用可以在使用或不使用商标为未决专利中公开的RamanNanoChip TM的增强型纳米结构化表面的情况下实现。 由于与传统增强表面相比，RamanNanoChip（TM）在SERS中提供了更高的灵敏度，因此现在可以实现更广泛的应用范围，并且可以实际实现，如本应用中所公开的。 此外，作为新的和改进的拉曼传感应用可以实现广泛的应用。 通过应用拉曼散射光谱分析，可以应用于识别未知化学成分来执行国土安全任务; 食品，药品和饮料安全; 早期疾病诊断环境监测; 工业过程监控，贵金属和宝石认证等

公开(公告)号：US20070229817A1

公开(公告)日：2007-10-04

申请号：US11754912

申请日：2007-05-29

Applicant: Hong Wang ,  Zhimin Liu

Inventor： Hong Wang ,  Zhimin Liu

IPC: G01J3/44

CPC classification number: G01N21/658

Abstract: A micro structure includes a silicon substrate, an adhesion layer on the silicon substrate, a bias layer on the adhesion layer, and structure layers on the adhesion layer. The two or more structure layers comprise different material compositions and a plurality of holes through at least two of the structure layers. Widths of the plurality of holes are in the range of 0.5-500 nm.

Abstract translation: 微结构包括硅衬底，硅衬底上的粘合层，粘合层上的偏置层和粘合层上的结构层。 两个或更多个结构层包括不同的材料组成和通过至少两个结构层的多个孔。 多个孔的宽度在0.5〜500nm的范围内。

公开(公告)号：US09807986B2

公开(公告)日：2017-11-07

申请号：US15083269

申请日：2016-03-28

Applicant: Rui Hou ,  Yuwei Wang ,  Huifang Liang ,  Zhanguo Zhang ,  Zhimin Liu ,  Binhao Zhang ,  Bixiang Zhang ,  Xiaoping Chen

Inventor： Rui Hou ,  Yuwei Wang ,  Huifang Liang ,  Zhanguo Zhang ,  Zhimin Liu ,  Binhao Zhang ,  Bixiang Zhang ,  Xiaoping Chen

IPC: A01K67/027 ,  A61K49/00 ,  G01N33/50 ,  C12N5/09 ,  C12N9/02

CPC classification number: A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  C12N5/0693 ,  C12N9/0069 ,  C12Y113/12007

Abstract: A method for establishing an animal model of hepatocellular carcinoma (HCC) bone metastasis, the method including: 1) establishing 97H and LM3 cell clones with stable expression of firefly luciferase (LUC); 2) allowing HCC cells to form bone metastasis in nude mice via intratibial injection; 3) reproducing HCC bone metastasis in nude mice via intracardiac injection of tumor cells; and 4) isolating a sub-population of tumor cells that targets metastasis to bone. The 97H and the LM3 are highly metastatic HCC cell lines transfected with luciferase gene. BALB/cA-nu mice are 4-5 weeks old and maintained in laminar flow cabinets under SPF conditions and received human care throughout an entire study. A cell number for intratibial injection is 0.5×106, and a cell number for intracardiac injection is 1×106.