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    Mutant delta-8 desaturase genes engineered by targeted mutagensis and their use in making polyunsaturated fatty acids
    1.
    发明授权
    Mutant delta-8 desaturase genes engineered by targeted mutagensis and their use in making polyunsaturated fatty acids 有权
    突变型delta-8去饱和酶基因由目标mutagensis工程化及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US08017838B2

    公开(公告)日：2011-09-13

    申请号：US11951697

    申请日：2007-12-06

    申请人： Howard G. Damude ,  Hongxian He ,  Der-ing Liao ,  Quinn Qun Zhu

    发明人： Howard G. Damude ,  Hongxian He ,  Der-ing Liao ,  Quinn Qun Zhu

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8247 ,  C12N9/0083

    摘要： The present invention relates to mutant delta-8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 omega-6, EDA] to dihomo-γ-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 omega-3, ETrA] to eicosatetraenoic acid [20:3 omega-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant delta-8 desaturases in plants and oleaginous yeast are disclosed.

    摘要翻译： 本发明涉及具有将二十碳二烯酸[20：2ω-6，EDA]转化为二高-γ-亚麻酸[20:3，DGLA]和/或二十碳三烯酸[...]的能力的突变体δ-8去饱和酶基因[ 20：3ω-3，ETrA]至二十碳四烯酸[20：3ω-3，ETA]。 公开了分离的核酸片段和重组构建体，其包含编码δ-8去饱和酶的片段以及使用植物中的这些突变型δ-8去饱和酶和油性酵母制备长链多不饱和脂肪酸（PUFA）的方法。

    Delta-5 desaturase and its use in making polyunsaturated fatty acids
    2.
    发明授权
    Delta-5 desaturase and its use in making polyunsaturated fatty acids 有权
    Delta-5去饱和酶及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US08049070B2

    公开(公告)日：2011-11-01

    申请号：US11749859

    申请日：2007-05-17

    申请人： Howard G. Damude ,  Quinn Qun Zhu

    发明人： Howard G. Damude ,  Quinn Qun Zhu

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C07H21/04

    CPC分类号： C12N9/0083

    摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-5 desaturase in plants and oleaginous yeast are disclosed.

    摘要翻译： 公开了分离的核酸片段和包含编码δ-5去饱和酶的片段的重组构建物，以及在植物和油质酵母中使用该δ-5去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。

    Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    7.
    发明授权
    Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效
    高山被孢霉溶血磷脂酸酰基转移酶同源物，用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US07524658B2

    公开(公告)日：2009-04-28

    申请号：US11717800

    申请日：2007-03-12

    申请人： Howard G. Damude ,  Zhixiong Xue

    发明人： Howard G. Damude ,  Zhixiong Xue

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    摘要： Lysophosphatidic acid acyltransferase (LPAAT) participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment (identified as “LPAAT2”) isolated from Mortierella alpina encoding a LPAAT homolog that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant LPAAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    摘要翻译： 溶血磷脂酰基转移酶（LPAAT）参与石油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 本申请提供了从编码LPAAT同源物的高山被孢霉分离的核酸片段（鉴定为“LPAAT2”），其适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的LPAAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母（Yarrowia lipolytica）中积累具有等于或大于C20链长的长链PUFA。