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16 条记录 (耗时 0.037 秒)

    Methods and compositions for targeting sequences of interest to the chloroplast
    3.
    发明授权
    Methods and compositions for targeting sequences of interest to the chloroplast 有权
    靶向叶绿体感兴趣序列的方法和组合物

    公开(公告)号：US09139842B2

    公开(公告)日：2015-09-22

    申请号：US13208960

    申请日：2011-08-12

    申请人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    发明人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    IPC分类号： C12N15/82 ,  C12N9/02

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

    摘要翻译： 提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上 活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。 还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。 组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。 这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。 进一步提供包含这些序列的细胞，植物细胞，植物和种子。 还提供了使用各种序列的方法。

    Method for detection of herbicides
    5.
    发明授权
    Method for detection of herbicides 失效
    检测除草剂的方法

    公开(公告)号：US5780254A

    公开(公告)日：1998-07-14

    申请号：US767363

    申请日：1996-12-18

    申请人： Daniel L. Siehl ,  Venkiteswaran Subramanian ,  Anne G. Toschi

    发明人： Daniel L. Siehl ,  Venkiteswaran Subramanian ,  Anne G. Toschi

    IPC分类号： C12Q1/48 ,  C12Q1/00 ,  C12Q1/32 ,  C12Q1/34

    CPC分类号： C12Q1/48

    摘要： This invention relates to novel screening methods for identifying compounds that specifically inhibit a metabolic target site or pathway in plants. Enzymes which are specifically affected by the novel screening method include plant purine biosynthetic pathway enzymes and particularly the enzymes involved in the conversion of IMP to AMP and the conversion of IMP to GMP. Further the invention relates to a method for screening and identifying potential novel compounds that function by the same mode of action as hydantocidin, and the compositions and method of use comprising said compounds.

    摘要翻译： 本发明涉及用于鉴定特异性抑制植物中代谢靶位点或途径的化合物的新型筛选方法。 受新型筛选方法特异性影响的酶包括植物嘌呤生物合成途径酶，特别是涉及IMP转化为AMP和将IMP转化为GMP的酶。 此外，本发明涉及筛选和鉴定通过与乙内酰素相同作用模式起作用的潜在新型化合物的方法，以及包含所述化合物的组合物和使用方法。

    Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast
    10.
    发明申请
    Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast 有权
    用于靶向叶绿体感兴趣的方法和组合物

    公开(公告)号：US20120042412A1

    公开(公告)日：2012-02-16

    申请号：US13208960

    申请日：2011-08-12

    申请人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    发明人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    IPC分类号： A01H1/06 ,  C12N15/82 ,  C12N5/10 ,  C12N9/96 ,  A01H5/10 ,  C07K19/00 ,  C12N9/02 ,  C12N15/62 ,  A01H5/00

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

    摘要翻译： 提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上 活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。 还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。 组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。 这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。 进一步提供包含这些序列的细胞，植物细胞，植物和种子。 还提供了使用各种序列的方法。