公开(公告)号：US20240400605A1

公开(公告)日：2024-12-05

申请号：US18806031

申请日：2024-08-15

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US12084474B2

公开(公告)日：2024-09-10

申请号：US16411913

申请日：2019-05-14

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2523/107 ,  C12Q2525/113 ,  C12Q2565/543

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US20250109437A1

公开(公告)日：2025-04-03

申请号：US18895767

申请日：2024-09-25

Applicant: ILLUMINA, INC.

Inventor： Jolene Lei ,  Jie Mao ,  Daniel Solis ,  Jelena Stojakovic

IPC: C12Q1/6874 ,  B01L3/00

Abstract: A kit for biological sequencing operations includes a flow cell including a plurality of depressions having a first diameter. The kit further includes a suspension including: a liquid carrier; and a plurality of functionalized nanostructures dispersed throughout the liquid carrier. Each of the plurality of functionalized nanostructures has a second diameter that is equal to or less than the first diameter. Each of the plurality of functionalized nanostructures includes: a nanostructure core; and a plurality of primers attached to the nanostructure core. The kit further includes a plurality of mechanical loading beads having a third diameter that is greater than the first diameter.

公开(公告)号：US20220090179A1

公开(公告)日：2022-03-24

申请号：US17472038

申请日：2021-09-10

Applicant: ILLUMINA, INC.

Inventor： Jie Mao ,  Randall Smith

IPC: C12Q1/6834 ,  G01N21/64 ,  C12Q1/6816

Abstract: A method for detecting an abasic site is provided. The method may include flowing a solution over a substrate having a plurality of oligonucleotides coupled thereto. At least one of the oligonucleotides includes an abasic site. The solution may include a fluorophore coupled to a reactive group. The method may include reacting the reactive group with the abasic site to couple the fluorophore to the abasic site; and detecting the abasic site using fluorescence from the fluorophore.

公开(公告)号：US20190352327A1

公开(公告)日：2019-11-21

申请号：US16411913

申请日：2019-05-14

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6876

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.