公开(公告)号：US20250011834A1

公开(公告)日：2025-01-09

申请号：US18753740

申请日：2024-06-25

Applicant: Illumina, Inc.

Inventor： Teodora Gal ,  Xavier von Hatten ,  Gianluca Artioli ,  Will Tovey ,  Nam Nguyen ,  Angelo La Rosa

IPC: C12Q1/6806 ,  B01J19/00 ,  C12Q1/6869 ,  C12Q1/6876

Abstract: This application relates to functionalized magnetic particles and methods of using magnetic particles to generate amplicons. In some examples, a method of modifying a magnetic particle comprising a first functional group includes contacting the magnetic particle with a first molecule comprising a polymer coupled to second and third functional groups, wherein the first functional group reacts with the second functional group to form a bond via which the polymer is coupled to the magnetic particle. The method may include contacting the magnetic particle with a second molecule, the second molecule comprising an oligonucleotide coupled to a fourth functional group, wherein the third functional group reacts with the fourth functional group to form a bond via which the oligonucleotide is coupled to the magnetic particle.

公开(公告)号：US20240150508A1

公开(公告)日：2024-05-09

申请号：US18482417

申请日：2023-10-06

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Nam Nguyen ,  Xavier von Hatten ,  Wayne George ,  Gianluca Artioli ,  Brian Mather ,  Johan Basuki ,  Shima Gholizadeh

IPC: C08F220/56 ,  C08J3/24 ,  C08J3/28

CPC classification number: C08F220/56 ,  C08J3/245 ,  C08J3/28 ,  C08F2810/20 ,  C08F2810/40 ,  C08J2333/26

Abstract: In some examples, novel photochemically-reversible hydrogels and nanogel particles are described comprising copolymer chains including at least one reactive alkene or reactive 1,4-diene end group capable of [2+2] or [2+2+2+2] photodimerization, respectively, at wavelengths >270 nm. In various examples, the photochemically-reversible hydrogels comprise copolymer chains including at least one —N3, —C≡CH or —CO2H end group for dual functionality and/or pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to photochemically-reversible hydrogels or nanogel particles reversibly bound to surfaces within a flow cell. After sequencing is complete, the photochemically-reversible hydrogel or nanogel particles is/are removable from the flow cell surfaces by irradiation, enabling the flow cell to be reusable.

公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US20250163505A1

公开(公告)日：2025-05-22

申请号：US18947955

申请日：2024-11-14

Applicant: Illumina, Inc.

Inventor： Nam Nguyen ,  Iuliana Petruta Maria ,  Andrew A. Brown ,  Wayne N. George ,  Xiaolin Wu ,  Xavier Von Hatten ,  Madushani Dharmarwardana

IPC: C12Q1/6874 ,  C08F134/04 ,  G01N33/58

Abstract: Embodiments of the present disclosure relate to compositions and methods for labeling of nucleotides, oligonucleotides, or polynucleotides with conjugated polymers or polymer dots. In particular, conjugated polymers or polymer dots can be used as detectable labels for nucleotides, oligonucleotides, or polynucleotides in sequencing by synthesis.

公开(公告)号：US20240117426A1

公开(公告)日：2024-04-11

申请号：US18469440

申请日：2023-09-18

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Nam Nguyen ,  Xavier von Hatten ,  Will Tovey ,  Andrew Brown ,  Wayne George ,  Eric Brustad ,  Gianluca Artioli

IPC: C12Q1/6874 ,  C08F120/06 ,  C08F120/56 ,  C08F120/60 ,  C08F122/38 ,  C08F138/02

CPC classification number: C12Q1/6874 ,  C08F120/06 ,  C08F120/56 ,  C08F120/60 ,  C08F122/385 ,  C08F138/02

Abstract: In some examples, novel nanogel particles are described having dual functionality, temperature responsiveness and pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to nanogel particles to form primer-grafted nanogel particles, and the primer-grafted nanogel particles are captured onto surfaces within a flow cell. Within flow cells such as used in SBS nucleic acid sequencing, each primer-grafted nanogel particle functions as a nano-well in the flow cell, thus eliminating the need for nano-wells in some examples.