公开(公告)号：US20240219835A1

公开(公告)日：2024-07-04

申请号：US18530025

申请日：2023-12-05

申请人： Illumina, Inc.

发明人： Tanmay Ghonge ,  David Prescott ,  Daniel Wright ,  Yekaterina Rokhlenko ,  Alexandra Szemjonov ,  Francesca Patel-Burrows ,  Gavriela Moskowitz

IPC分类号： G03F7/11 ,  B01J19/00 ,  B01L3/00 ,  G03F7/038

CPC分类号： G03F7/11 ,  B01J19/0046 ,  B01L3/502707 ,  G03F7/038 ,  B01J2219/00722 ,  B01L2200/12

摘要： Examples of flow cells include substrates. Embodiments of the present disclosure also relate to methods of fabricating flow cell substrates. Some example workflows exploit light blocking properties of an imprint layer such that the process does not include etch steps. Such processes may be used to create substrates compatible with simultaneous paired-end sequencing methods.

公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

申请人： ILLUMINA, INC.

发明人： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC分类号： B01L3/00

CPC分类号： B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

摘要： An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US20240207839A1

公开(公告)日：2024-06-27

申请号：US18542470

申请日：2023-12-15

申请人： ILLUMINA, INC.

发明人： Johan Sebastian Basuki ,  Brian D. Mather ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov

IPC分类号： B01L3/00

CPC分类号： B01L3/502 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/165

摘要： An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a composite that includes a thermo-responsive polymer and a photo-thermal additive.