公开(公告)号：US20240219835A1

公开(公告)日：2024-07-04

申请号：US18530025

申请日：2023-12-05

Applicant: Illumina, Inc.

Inventor： Tanmay Ghonge ,  David Prescott ,  Daniel Wright ,  Yekaterina Rokhlenko ,  Alexandra Szemjonov ,  Francesca Patel-Burrows ,  Gavriela Moskowitz

IPC: G03F7/11 ,  B01J19/00 ,  B01L3/00 ,  G03F7/038

CPC classification number: G03F7/11 ,  B01J19/0046 ,  B01L3/502707 ,  G03F7/038 ,  B01J2219/00722 ,  B01L2200/12

Abstract: Examples of flow cells include substrates. Embodiments of the present disclosure also relate to methods of fabricating flow cell substrates. Some example workflows exploit light blocking properties of an imprint layer such that the process does not include etch steps. Such processes may be used to create substrates compatible with simultaneous paired-end sequencing methods.

公开(公告)号：US20240375075A1

公开(公告)日：2024-11-14

申请号：US18658753

申请日：2024-05-08

Applicant: ILLUMINA, INC.

Inventor： Francesca Patel-Burrows ,  Alexandre Richez ,  Alexandra Szemjonov

IPC: B01J19/00

Abstract: In an example of a method of making a flow cell, a functional material is deposited over a resin layer including depressions separated by interstitial regions. The resin layer includes an ultraviolet (UV) light blocking additive. The depressions overlie a first portion of the resin layer having a first thickness, and the interstitial regions overlie a second portion of the resin layer having a second thickness that is greater than the first thickness. The functional material is susceptible to interaction with the resin layer when exposed to UV light. A predetermined UV light dosage is directed through the resin layer, whereby the functional material within the depressions is exposed to the UV light and attaches to the resin layer within the depressions. The functional material overlying the interstitial regions is blocked from being exposed to the UV light by the second resin portion.

公开(公告)号：US20250010291A1

公开(公告)日：2025-01-09

申请号：US18753229

申请日：2024-06-25

Applicant: Illumina, Inc.

Inventor： Daniel Wright ,  Alexandra Szemjonov ,  Wayne N. George ,  Francesca Patel-Burrows

IPC: B01L3/00

Abstract: Embodiments of the present disclosure relate to patterned substrates with functionalized surface such as flow cells, as well as methods of fabricating the patterned substrate. In particular, patterned substrates of the present disclosure may be prepared using two or more imprint resin layers, one of which acts as a photomask for the photoresist during substrate patterning, without the need of any metallic photomask. Embodiments of the patterned substrate may be used for simultaneous paired-end sequencing methods.

公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US20240207839A1

公开(公告)日：2024-06-27

申请号：US18542470

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Johan Sebastian Basuki ,  Brian D. Mather ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/165

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a composite that includes a thermo-responsive polymer and a photo-thermal additive.