公开(公告)号：US20240026348A1

公开(公告)日：2024-01-25

申请号：US18476719

申请日：2023-09-28

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Susan C. Verity ,  Robert Scott Kuersten ,  Niall Anthony Gormley ,  Andrew B. Kennedy ,  Sarah E. Shultzaberger ,  Andrew Slatter ,  Emma Bell ,  Sebastien Georg Gabriel Ricoult ,  Grace DeSantis ,  Fiona Kaper ,  Han-Yu Chuang ,  Oliver Jon Miller ,  Jason Richard Betley ,  Stephen M. Gross ,  Mats Ekstrand

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12N15/1068

Abstract: Materials and methods for preparing nucleic acid libraries for next-generation sequencing are described herein. A variety of approaches are described relating to the use of unique molecular identifiers with transposon-based technology in the preparation of sequencing libraries. Also described herein are sequencing materials and methods for identifying and correcting amplification and sequencing errors.

公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US20240207839A1

公开(公告)日：2024-06-27

申请号：US18542470

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Johan Sebastian Basuki ,  Brian D. Mather ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/165

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a composite that includes a thermo-responsive polymer and a photo-thermal additive.

公开(公告)号：US20230279469A1

公开(公告)日：2023-09-07

申请号：US18146320

申请日：2022-12-23

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC. ,  Illumina Singapore Pte. Ltd.

Inventor： Johan Sebastian Basuki ,  Jonathan Mark Boutell ,  Jeffrey S. Fisher ,  Louise Jane Fraser ,  Wayne N. George ,  Niall Anthony Gormley ,  David Jones ,  Xiaoyu Ma ,  Maria Ines Martins Vitoriano ,  Zhong Mei ,  Oliver Jon Miller ,  Andrew Price ,  Sebastien Georg Gabriel Ricoult ,  Vicki S. Thomson ,  Jacqueline C. Weir ,  Xiaoy Ma ,  Weihua Chang ,  Hui Han

IPC: C12Q1/6806 ,  C12N15/10 ,  B01L3/00

CPC classification number: C12Q1/6806 ,  B01L3/502761 ,  C12N15/1065 ,  B01L2200/0647 ,  B01L2300/0893

Abstract: An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.

公开(公告)号：US20200325467A1

公开(公告)日：2020-10-15

申请号：US16626221

申请日：2018-06-28

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Brian D. Mather ,  Cyril Delattre ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Pallavi Daggumati ,  Behnam Javanmardi ,  Filiz Gorpe-Yasar ,  Sebastien Georg Gabriel Ricoult ,  Xavier von Hatten ,  Daniel Leonard Fuller

IPC: C12N15/10 ,  C08F226/10

Abstract: An example of a nucleic acid extraction material includes a substrate. The nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. The polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.