中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

28 records (took 0.016 seconds)

    Flow cells and methods for making the same
    1.
    发明授权
    Flow cells and methods for making the same 有权

    公开(公告)号：US12298665B2

    公开(公告)日：2025-05-13

    申请号：US17486568

    申请日：2021-09-27

    Applicant: ILLUMINA, INC.

    Inventor： Sahngki Hong ,  Lewis J. Kraft

    IPC: C08F2/46 ,  C08F2/50 ,  C08G61/04 ,  G03F7/038 ,  G03F7/039 ,  G03F7/20 ,  G03F7/34 ,  G03F7/38

    Abstract: An example flow cell includes a multi-layer stack including a transparent base support; a patterned sacrificial layer over the transparent base support; and a transparent layer over the patterned sacrificial layer. The flow cell further includes first and second functionalized layers over different portions of the transparent layer, wherein at least one of the first and second functionalized layers aligns with a pattern of the patterned sacrificial layer; and first and second primer sets respectively attached to the first and second functionalized layer.

    CAPTURING AND AMPLIFYING POLYNUCLEOTIDES USING PARTICLES
    4.
    发明申请
    CAPTURING AND AMPLIFYING POLYNUCLEOTIDES USING PARTICLES 有权

    公开(公告)号：US20250011862A1

    公开(公告)日：2025-01-09

    申请号：US18753228

    申请日：2024-06-25

    Applicant: Illumina, Inc.

    Inventor： Sahngki Hong ,  Fei Shen ,  Lewis Kraft

    IPC: C12Q1/6874 ,  C12Q1/6844

    Abstract: In some examples, a method for capturing and amplifying a polynucleotide includes capturing the polynucleotide at a particle comprising a first region and a second region. The first region may include a first moiety that captures the polynucleotide. The second region may include a plurality of amplification primers and have a surface area which is substantially larger than the surface area of the first portion. The method includes using the plurality of amplification primers to amplify the captured polynucleotide.

    FLOW CELLS AND METHODS FOR MAKING THE SAME
    7.
    发明申请
    FLOW CELLS AND METHODS FOR MAKING THE SAME 有权

    公开(公告)号：US20230137978A1

    公开(公告)日：2023-05-04

    申请号：US17739976

    申请日：2022-05-09

    Applicant: ILLUMINA, INC.

    Inventor： Jeffrey S. Fisher ,  Anthony Flannery ,  Sahngki Hong ,  Brinda Kodira Cariappa ,  Lewis J. Kraft ,  Brian D. Mather

    IPC: B01L3/00 ,  G03F7/038 ,  G03F7/039 ,  G03F7/00

    Abstract: A metal film is formed over a resin layer including a plurality of multi-depth depressions (MDP) separated by interstitial regions, each MDP including a deep portion and an adjacent shallow portion. A sacrificial layer is formed over the metal film. The sacrificial layer and metal film are sequentially dry etched to expose a resin layer surface at the shallow portion and interstitial regions. Resin layer portions are removed i) at the shallow portion to form a depression region having a surface directly adjacent to a surface at the deep portion and ii) at the interstitial regions to form new interstitial regions surrounding the deep portion and the depression region. First functionalized layer is deposited over the metal film, depression region, and new interstitial regions. The metal film is removed from the deep portion. Second functionalized layer is deposited over the surface at the deep portion. New interstitial regions are polished.

    FLOW CELLS AND METHODS FOR MAKING THE SAME
    8.
    发明申请
    FLOW CELLS AND METHODS FOR MAKING THE SAME 有权

    公开(公告)号：US20220382147A1

    公开(公告)日：2022-12-01

    申请号：US17825989

    申请日：2022-05-26

    Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

    Inventor： Brinda Kodira Cariappa ,  Wayne N. George ,  Sahngki Hong ,  Lewis J. Kraft

    IPC: G03F7/00 ,  B01J19/00 ,  G03F7/038 ,  G03F7/20

    Abstract: In an example of a method for making a flow cell, a light sensitive material is deposited over a resin layer including depressions separated by interstitial regions, wherein the depressions overlie a first resin portion having a first thickness and the interstitial regions overlie a second resin portion having a second thickness that is greater than the first thickness. A predetermined ultraviolet light dosage that is based on the first and second thicknesses is directed through the resin layer, whereby the light sensitive material overlying the depressions is exposed to ultraviolet light and the second resin portion absorbs the ultraviolet light, thereby defining an altered light sensitive material at a first predetermined region over the resin layer. The altered light sensitive material is utilized to generate a functionalized layer at the first predetermined region or at a second predetermined region over the resin layer.

    FLOW CELL COATING METHODS
    9.
    发明申请
    FLOW CELL COATING METHODS 有权

    公开(公告)号：US20220187710A1

    公开(公告)日：2022-06-16

    申请号：US17550681

    申请日：2021-12-14

    Applicant: ILLUMINA, INC.

    Inventor： Neil Brahma ,  Tyler J. Dill ,  Michelle Kate Fu ,  Brian A. Hanos ,  Sahngki Hong ,  Brinda Kodira Cariappa ,  Lewis J. Kraft

    IPC: G03F7/20 ,  G01N21/64 ,  G03F7/16

    Abstract: In an example method, a water-soluble protective coating solution is applied over a bonding region and either i) a patterned region of a patterned structure or ii) a lane region of a non-patterned structure. The patterned region includes depressions having at least a polymeric hydrogel therein, and interstitial regions separating the depressions. The lane region includes a lane having at least the polymeric hydrogel therein. The water-soluble protective coating solution is dried to form a solid coating or a gel coating over the bonding region and over either i) the patterned region or ii) the lane region. Portions of the solid coating or the gel coating are selectively removed from the bonding region while leaving other portions of the solid coating or the gel coating over either i) the patterned region or ii) the lane region.

Patent Agency Ranking