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    GENOMIC LIBRARY PREPARATION AND TARGETED EPIGENETIC ASSAYS USING CAS-GRNA RIBONUCLEOPROTEINS
    2.
    发明公开
    GENOMIC LIBRARY PREPARATION AND TARGETED EPIGENETIC ASSAYS USING CAS-GRNA RIBONUCLEOPROTEINS 审中-公开

    公开(公告)号：US20240287504A1

    公开(公告)日：2024-08-29

    申请号：US18549344

    申请日：2022-03-08

    申请人： Illumina, Inc. ,  Illumina Cambridge Limited

    发明人： Andrew Kennedy ,  Sarah Shultzaberger ,  Emma Bell ,  Oliver Miller ,  Kim Schneider ,  Esther Musgrave-Brown ,  Niall Gormley ,  Andrew Slatter ,  Feng Chen

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1065

    摘要： Genomic library preparation using Cas-gRNA RNPs, and targeted epigenetic assays, are provided herein. Some compositions include, from a first species, substantially only single-stranded polynucleotides; from a second species, substantially only double-stranded polynucleotides; and amplification primers ligated to ends of the second double-stranded polynucleotides and substantially not ligated to any ends of the first double-stranded polynucleotides. Some compositions include first and second molecules of a target polynucleotide having a sequence, the first molecule having a first end at a first subsequence, the second molecule having a first end at a second subsequence, wherein the first subsequence only partially overlaps with the second subsequence. Some examples provide a composition that includes a target polynucleotide and a first fusion protein including a Cas-gRNA RNP coupled to a transposase having an amplification adapter coupled thereto. The Cas-gRNA RNP may be hybridized to a subsequence in the target polynucleotide.

    KITS FOR GENOTYPING
    4.
    发明申请
    KITS FOR GENOTYPING 有权

    公开(公告)号：US20210262028A1

    公开(公告)日：2021-08-26

    申请号：US17185730

    申请日：2021-02-25

    申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

    发明人： Andrew Slatter ,  Eric Hans Vermaas

    IPC分类号： C12Q1/6876 ,  C12Q1/6869

    摘要： An example of a kit includes a flow cell and a genotyping probe fluid. The flow cell includes a substrate, and first and second capture primers attached to the substrate. The genotyping probe fluid includes a liquid carrier, and a genotyping oligonucleotide in the liquid carrier. The genotyping oligonucleotide includes a first primer sequence; a probe sequence that is representative of a target genotyping locus; a restriction endonuclease site; and a second primer sequence that is at least partially complementary to the second capture primer.

    Separating polynucleotide fragments
    8.
    发明授权
    Separating polynucleotide fragments 有权

    公开(公告)号：US11760994B2

    公开(公告)日：2023-09-19

    申请号：US17358892

    申请日：2021-06-25

    申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

    发明人： Maria Candelaria Rogert Bacigalupo ,  Frank Steemers ,  Jeffrey Fisher ,  Andrew Slatter ,  Lewis Kraft ,  Niall Gormley ,  M. Shane Bowen

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1065 ,  C12N15/1093

    摘要： Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.