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公开(公告)号：US11291998B2
公开(公告)日：2022-04-05
申请号：US17165757
申请日：2021-02-02
发明人： Wesley A. Cox-Muranami , Darren Robert Segale , Oliver Jon Miller , Jennifer Olivia Foley , Tarun Kumar Khurana , Paul Crivelli , Gary Watts
摘要： An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.
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公开(公告)号：US11179724B2
公开(公告)日：2021-11-23
申请号：US16127882
申请日：2018-09-11
发明人： Bradley Kent Drews , Gudrun Stengel , James Christopher Blake , Mohammed Kafeel Ahamed , Michael Steven Becker , Michael Dangelo , Mark J. Nibbe , Daniel L. Fuller , Oliver Jon Miller
摘要： The assembly includes a docking console and a manifold. The docking console includes a cartridge support surface having a first end and a second end. The manifold has one or more wells defined therein. The docking console further includes a manifold retention bracket to releasably hold the manifold against a fluid cartridge supported on the cartridge support surface at an interface position such that the one or more wells are in fluid communication with the fluid cartridge and a biased seal bar to press the fluid cartridge against the manifold held by the manifold retention bracket. A hydrophilic porous frit disposed within at least one of the wells and is to permit liquid to flow through the outlet aperture but prevent gas from passing through the outlet aperture.
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公开(公告)号：US20210154667A1
公开(公告)日：2021-05-27
申请号：US17165757
申请日：2021-02-02
发明人： Wesley A. Cox-Muranami , Darren Robert Segale , Oliver Jon Miller , Jennifer Olivia Foley , Tarun Kumar Khurana , Paul Crivelli , Gary Watts
摘要： An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.
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公开(公告)号：US20230279469A1
公开(公告)日：2023-09-07
申请号：US18146320
申请日：2022-12-23
发明人： Johan Sebastian Basuki , Jonathan Mark Boutell , Jeffrey S. Fisher , Louise Jane Fraser , Wayne N. George , Niall Anthony Gormley , David Jones , Xiaoyu Ma , Maria Ines Martins Vitoriano , Zhong Mei , Oliver Jon Miller , Andrew Price , Sebastien Georg Gabriel Ricoult , Vicki S. Thomson , Jacqueline C. Weir , Xiaoy Ma , Weihua Chang , Hui Han
IPC分类号： C12Q1/6806 , C12N15/10 , B01L3/00
CPC分类号： C12Q1/6806 , B01L3/502761 , C12N15/1065 , B01L2200/0647 , B01L2300/0893
摘要： An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.
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公开(公告)号：US20210164027A1
公开(公告)日：2021-06-03
申请号：US17267107
申请日：2019-08-13
发明人： Andrew F. Slatter , Junhua Zhao , Grace DeSantis , Stephen M. Gross , Hayley Marianne Hogan , Jian-Sen Li , Oliver Jon Miller
IPC分类号： C12Q1/6832 , C12Q1/6869
摘要： This disclosure describes a hybridization buffer including a crowding agent, a method that includes using the hybridization buffer, and a kit including the hybridization buffer. This disclosure also describes blockers for use in hybrid capture methods, methods of using those blockers, and a kit including those blockers. Additionally, this disclosure describes a method of hybrid capture that does not include amplifying the library members using PCR prior to sequencing the library members.
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公开(公告)号：US20230314322A1
公开(公告)日：2023-10-05
申请号：US18190809
申请日：2023-03-27
IPC分类号： G01N21/64 , C12Q1/6874
CPC分类号： G01N21/6428 , C12Q1/6874
摘要： The application relates to DNA sequencing systems and methods. Systems and methods for determining the nucleotide sequence of a polynucleotide include attaching three different fluorescent dyes to three different nucleotides during incorporation. In particular, long Stokes shifted dyes may be used to determine the sequence of polynucleotides in a sequencing by synthesis system.
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公开(公告)号：US20230212667A1
公开(公告)日：2023-07-06
申请号：US18089867
申请日：2022-12-28
发明人： Jonathan Mark Boutell , Eli M. Carrami , Pietro Gatti Lafranconi , Philip Balding , Oliver Jon Miller , Kay Klausing , Justin Robbins , Xiaolin Wu
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874 , C12Q1/6806
摘要： Polynucleotide sequencing methods for sequencing one or more polynucleotide templates use primers bound to a surface as sequencing primers. The surface primers may include at least a portion of a surface oligonucleotide used during cluster formation. The sequencing methods may be used for single stranded sequencing or double stranded sequencing. Double stranded sequencing methods may employ an enzyme that has nick-translation activity. A kit includes all the reagents needed for sequencing does not include sequencing primers. The kit may be used to accomplish the sequencing methods of the present disclosure.
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公开(公告)号：US11975328B2
公开(公告)日：2024-05-07
申请号：US17507254
申请日：2021-10-21
发明人： Bradley Kent Drews , Gudrun Stengel , James Christopher Blake , Mohammed Kafeel Ahamed , Michael Steven Becker , Michael Dangelo , Mark J. Nibbe , Daniel L. Fuller , Oliver Jon Miller
CPC分类号： B01L3/50853 , B01L3/502715 , B01L9/523 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0684 , B01L2200/0689 , B01L2300/041 , B01L2300/047 , B01L2300/048 , B01L2300/0681 , B01L2300/0819 , B01L2300/0829
摘要： The assembly includes a docking console and a manifold. The docking console includes a cartridge support surface having a first end and a second end. The manifold has one or more wells defined therein. The docking console further includes a manifold retention bracket to releasably hold the manifold against a fluid cartridge supported on the cartridge support surface at an interface position such that the one or more wells are in fluid communication with the fluid cartridge and a biased seal bar to press the fluid cartridge against the manifold held by the manifold retention bracket. A hydrophilic porous frit disposed within at least one of the wells and is to permit liquid to flow through the outlet aperture but prevent gas from passing through the outlet aperture.
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公开(公告)号：US20240026348A1
公开(公告)日：2024-01-25
申请号：US18476719
申请日：2023-09-28
发明人： Susan C. Verity , Robert Scott Kuersten , Niall Anthony Gormley , Andrew B. Kennedy , Sarah E. Shultzaberger , Andrew Slatter , Emma Bell , Sebastien Georg Gabriel Ricoult , Grace DeSantis , Fiona Kaper , Han-Yu Chuang , Oliver Jon Miller , Jason Richard Betley , Stephen M. Gross , Mats Ekstrand
IPC分类号： C12N15/10
CPC分类号： C12N15/1065 , C12N15/1068
摘要： Materials and methods for preparing nucleic acid libraries for next-generation sequencing are described herein. A variety of approaches are described relating to the use of unique molecular identifiers with transposon-based technology in the preparation of sequencing libraries. Also described herein are sequencing materials and methods for identifying and correcting amplification and sequencing errors.
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公开(公告)号：US10953399B2
公开(公告)日：2021-03-23
申请号：US16244776
申请日：2019-01-10
发明人： Wesley A. Cox-Muranami , Darren Robert Segale , Oliver Jon Miller , Jennifer Olivia Foley , Tarun Kumar Khurana , Paul Crivelli , Gary Watts
摘要： An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.
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