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公开(公告)号：US11634697B2
公开(公告)日：2023-04-25
申请号：US17226393
申请日：2021-04-09
发明人： Kay Klausing , Hamed Tabatabaei Ghomi , Misha Golynskiy , Saurabh Nirantar , Seth McDonald , Sergio Peisajovich
IPC分类号： C12N9/12
摘要： Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
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公开(公告)号：US20210348141A1
公开(公告)日：2021-11-11
申请号：US17226393
申请日：2021-04-09
发明人： Kay Klausing , Hamed Tabatabaei Ghomi , Misha Golynskiy , Saurabh Nirantar , Seth McDonald , Sergio Peisajovich
IPC分类号： C12N9/12
摘要： Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
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公开(公告)号：US20230212667A1
公开(公告)日：2023-07-06
申请号：US18089867
申请日：2022-12-28
发明人： Jonathan Mark Boutell , Eli M. Carrami , Pietro Gatti Lafranconi , Philip Balding , Oliver Jon Miller , Kay Klausing , Justin Robbins , Xiaolin Wu
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874 , C12Q1/6806
摘要： Polynucleotide sequencing methods for sequencing one or more polynucleotide templates use primers bound to a surface as sequencing primers. The surface primers may include at least a portion of a surface oligonucleotide used during cluster formation. The sequencing methods may be used for single stranded sequencing or double stranded sequencing. Double stranded sequencing methods may employ an enzyme that has nick-translation activity. A kit includes all the reagents needed for sequencing does not include sequencing primers. The kit may be used to accomplish the sequencing methods of the present disclosure.
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公开(公告)号：US20230047225A1
公开(公告)日：2023-02-16
申请号：US17829866
申请日：2022-06-01
发明人： Misha Golynskiy , Mariko Matsuura , Saurabh Nirantar , Hamed Tabatabaei Ghomi , Seth McDonald , Ryan Craig , Sergio Peisajovich , Kyrie Johnson , Kay Klausing , Eric Murtfeldt , Alexandra Exner
IPC分类号： C12N9/12 , C12Q1/6874
摘要： Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.
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公开(公告)号：US12104182B2
公开(公告)日：2024-10-01
申请号：US18120639
申请日：2023-03-13
发明人： Kay Klausing , Hamed Tabatabaei Ghomi , Misha Golynskiy , Saurabh Nirantar , Seth McDonald , Sergio Peisajovich
IPC分类号： C12N9/12
CPC分类号： C12N9/1252 , C12Y207/07007
摘要： Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
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公开(公告)号：US20230357731A1
公开(公告)日：2023-11-09
申请号：US18120639
申请日：2023-03-13
发明人： Kay Klausing , Hamed Tabatabaei Ghomi , Misha Golynskiy , Saurabh Nirantar , Seth McDonald , Sergio Peisajovich
IPC分类号： C12N9/12
CPC分类号： C12N9/1252 , C12Y207/07007
摘要： Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
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公开(公告)号：US11001816B2
公开(公告)日：2021-05-11
申请号：US16703569
申请日：2019-12-04
发明人： Kay Klausing , Hamed Tabatabaei Ghomi , Misha Golynskiy , Saurabh Nirantar , Seth McDonald , Sergio Peisajovich
IPC分类号： C12N9/12
摘要： Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
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公开(公告)号：US20200181587A1
公开(公告)日：2020-06-11
申请号：US16703569
申请日：2019-12-04
发明人： Kay Klausing , Hamed Tabatabael Ghomi , Misha Golynskiy , Saurabh Nirantar , Seth McDonald , Sergio Peisajovich
IPC分类号： C12N9/12
摘要： Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
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公开(公告)号：US20240141428A1
公开(公告)日：2024-05-02
申请号：US18489788
申请日：2023-10-18
申请人： Illumina, Inc.
发明人： Robert C. Kain , Xiaohai Liu , Wenyi Feng , Bernard Hirschbein , Helmy A. Eltoukhy , Xiaolin Wu , Geoffrey Paul Smith , Jonathan Mark Boutell , Thomas Joseph , Randall Smith , Min-Jui Richard Shen , Carolyn Tregidgo , Kay Klausing
IPC分类号： C12Q1/6874 , C12Q1/6869
CPC分类号： C12Q1/6874 , C12Q1/6869
摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
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10.发明公开公开(公告)号：US20230272457A1
公开(公告)日：2023-08-31
申请号：US18179026
申请日：2023-03-06
申请人： ILLUMINA, INC.
发明人： Boyan Boyanov , Liangliang Qiang , Kevin L. Gunderson , Kay Klausing , Lea Pickering , Cyril Delattre , Tarun Khurana
IPC分类号： C12Q1/6818 , C12Q1/6874 , C12Q1/6816
CPC分类号： C12Q1/6818 , C12Q1/6874 , C12Q1/6816 , C12Q2533/101 , C12Q2535/113 , C12Q2535/122 , C12Q2563/103 , C12Q2563/125
摘要： Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.
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