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公开(公告)号：US20230235410A1
公开(公告)日：2023-07-27
申请号：US18089565
申请日：2022-12-28
Applicant: Industrial Technology Research Institute
Inventor： Jian-Hao Li , Hui-Chu Hsieh , Po-Chang Chen , Pei-Shin Jiang , Chih-Lung Lin
IPC: C12Q1/6886
CPC classification number: C12Q1/6886 , C12Q2600/158
Abstract: An identification system of circulating biomarkers for cancer detection, a development method of circulating biomarkers for cancer detection, a cancer detection method and a kit are provided in the present disclosure, and the development method includes the following steps. Expression levels of multiple genes in normal tissue samples and tumor tissue samples are identified, and genes with high expression levels in the tumor tissue samples are selected. Afterwards, a weight of each human tissue’s contribution to plasma exosomes is calculated using tissue-specific genes and group-enriched genes. Next, expression levels of plasma exosome genes of healthy people and cancer patients are compared by an overlapping index, and circulating biomarkers and combinations thereof suitable for detection and evaluation of plasma exosomes are selected.
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公开(公告)号：US11959101B2
公开(公告)日：2024-04-16
申请号：US17535694
申请日：2021-11-26
Applicant: Industrial Technology Research Institute
Inventor： Ting-Hsuan Chen , Kuo-Hsing Wen , Ya-Hui Chiu , Nien-Tzu Chou , Ching-Fang Lu , Cheng-Tai Chen , Ting-Shuo Chen , Pei-Shin Jiang
Abstract: A cell activation reactor and a cell activation method are provided. The cell activation reactor includes a body, a rotating part, an upper cover, a microporous film, and multiple baffles. The body has an accommodating space, which is suitable for accommodating multiple cells and multiple magnetic beads. The rotating part is disposed in the accommodating space and includes multiple impellers. The microporous film is disposed in the accommodating space and covers multiple holes of the accommodating space. The baffles are disposed in the body. When the rotating part is driven to rotate, the interaction between the baffles and the impellers separates the cells and the magnetic beads.
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公开(公告)号：US20210214780A1
公开(公告)日：2021-07-15
申请号：US17134502
申请日：2020-12-28
Applicant: Industrial Technology Research Institute
Inventor： Pei-Shin Jiang , Jenn-Yeh Fann , Hung-Chi Chien , Yu-Yu Lin , Chih-Lung Lin
IPC: C12Q1/6855 , C12Q1/6869
Abstract: Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.
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公开(公告)号：US11859176B2
公开(公告)日：2024-01-02
申请号：US16228763
申请日：2018-12-21
Inventor： Cheng-Tai Chen , Chien-An Chen , Wen-Ting Chiang , Su-Feng Chiu , Pei-Shin Jiang , Jih-Luh Tang , Chien-Ting Lin , Xuan-Hui Lin
IPC: C12N5/00 , C12N13/00 , G01N33/543 , C12N5/0783 , C12N5/078 , A61K9/14
CPC classification number: C12N13/00 , C12N5/0634 , C12N5/0636 , C12N5/0646 , G01N33/54333 , C12N2501/505 , C12N2501/515 , C12N2501/998 , C12N2529/00 , C12N2533/10 , C12N2533/30 , C12N2535/00 , C12N2535/10
Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.
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公开(公告)号：US20190241936A1
公开(公告)日：2019-08-08
申请号：US16227735
申请日：2018-12-20
Applicant: Industrial Technology Research Institute
Inventor： Tseng-Huang Liu , Chia-Ying Tang , Pei-Shin Jiang
IPC: C12Q1/689 , C07K17/02 , C12Q1/6895 , G01N33/543
CPC classification number: C12Q1/689 , C07K17/02 , C12Q1/04 , C12Q1/37 , C12Q1/6895 , G01N33/523 , G01N33/54306
Abstract: A test strip for microorganism detection includes: a porous substrate, a surface of which a surface has a first color; and a microorganism detection composition, which is at least adhered to a part of a surface of the porous substrate or at least adhered to a part of a surface of the porous substrate and a part of an interior of the porous substrate. The microorganism detection composition includes: a plurality of colored particles having a second color different than the first color; and a plurality of specific peptides immobilized on the surfaces of the plurality of colored particles, in which the plurality of specific peptides connect the plurality of colored particles to each other to make the plurality of colored particles aggregate to cover the part of the surface of the porous substrate and present the second color on the part of the surface of the porous substrate.
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Patent Agency Ranking
公开(公告)号：US20190206598A1
公开(公告)日：2019-07-04
申请号：US16226651
申请日：2018-12-20
Applicant: Industrial Technology Research Institute
Inventor： Chien-An Chen , Cheng-Tai Chen , Pei-Shin Jiang
CPC classification number: H01F1/26 , G01N33/5434 , H01F1/0054 , H01F1/37
Abstract: Described herein is a magnetic particle, which includes a knobby copolymer core, a polymer layer, a magnetic substance layer and a silicon-based layer. The polymer layer covers the knobby copolymer core and has at least one functional group. In addition, the magnetic substance layer covers the polymer layer and the silicon-based layer covers the magnetic substance layer. Furthermore, a manufacturing method of the magnetic particle is also described.
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公开(公告)号：US20190203195A1
公开(公告)日：2019-07-04
申请号：US16228763
申请日：2018-12-21
Inventor： Cheng-Tai Chen , Chien-An Chen , Wen-Ting Chiang , Su-Feng Chiu , Pei-Shin Jiang , Jih-Luh Tang , Chien-Ting Lin , Xuan-Hui Lin
CPC classification number: C12N13/00 , C12N5/0634 , C12N5/0636 , C12N2501/505 , C12N2501/515 , C12N2501/998 , C12N2529/00 , C12N2533/10 , C12N2533/30 , C12N2535/00 , C12N2535/10 , G01N33/54333
Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.
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公开(公告)号：US09664664B2
公开(公告)日：2017-05-30
申请号：US14582844
申请日：2014-12-24
Applicant: Industrial Technology Research Institute
Inventor： Cheng-Tai Chen , Pei-Shin Jiang
CPC classification number: G01N33/20 , G01N21/6428 , G01N31/22 , G01N33/54346 , G01N33/553 , G01N33/587 , G01N33/84 , G01N2021/7786
Abstract: A method for detecting metal ions and chemical/biochemical molecules is provided. The method includes providing a probe, wherein the probe includes: a gold nanocluster; a reducing agent and a chelating agent partially capped on a surface of the gold nanocluster, wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9. The probe may interact with several metal ions of an aqueous solution to produce different changes of fluorescent spectra. Chemical/biochemical molecules can be detected by the fluorescent spectra difference caused by the interaction between the metal ions and the chemical/biochemical molecules.
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9.发明授权Kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence 有权用于检测靶核苷酸序列中特定区域的突变和/或多态性的试剂盒公开(公告)号：US09228231B2
公开(公告)日：2016-01-05
申请号：US13725273
申请日：2012-12-21
Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
Inventor： Pei-Shin Jiang , Tzu-Hui Wu , Chia-Chun Chen , Su-Jan Lee , Chien-An Chen , Chien-Ming Hsu , Chung-Ya Liao , Yu-Yu Lin
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , C12Q1/6827 , C12Q2600/156
Abstract: One embodiment of the disclosure provides a kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence, including: at least one first primer consisting of a first segment and a second segment, wherein the first segment is a complementary strand of a first sequence and the second segment is a second sequence, and the 3′ end of the first segment connects to the 5′ end of the second segment; a second primer being a third sequence; at least one third primer consisting of a third segment and a fourth segment, wherein the third segment is a fourth sequence and the fourth segment is a complementary strand of a fifth sequence, and the 3′ end of the third segment connects to the 5′ end of the fourth segment; and a fourth primer being a complementary strand of a sixth sequence, wherein the specific region includes rs1799853, rs1057910, rs2108622, rs9923231 and rs9934438.
Abstract translation: 本公开的一个实施方案提供了用于检测靶核苷酸序列中特定区域的突变和/或多态性的试剂盒，其包括：至少一种由第一片段和第二片段组成的第一引物，其中第一片段是互补的 第二段是第二序列，第一段的3'端连接到第二段的5'端; 第二引物是第三序列; 至少一个第三引物由第三片段和第四片段组成，其中第三片段是第四序列，第四片段是第五序列的互补链，第三片段的3'末端连接到5' 第四段结束; 第四引物是第六序列的互补链，其中特异性区包括rs1799853，rs1057910，rs2108622，rs9923231和rs9934438。
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公开(公告)号：US20140186894A1
公开(公告)日：2014-07-03
申请号：US14138155
申请日：2013-12-23
Applicant: Industrial Technology Research Institute
Inventor： Tseng-Huang Liu , Pei-Shin Jiang , Chih-Lung Lin , Su-Jan Lee , Chao-Hung Kao
CPC classification number: C12N9/1252
Abstract: Isolated DNA polymerase and the mutant DNA polymerases thereof are provided. The DNA polymerases have good thermostability.
Abstract translation: 提供了分离的DNA聚合酶及其突变型DNA聚合酶。 DNA聚合酶具有良好的热稳定性。
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