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    HIV-2 transmembrane glycoprotein homodimer (gp 80)
    2.
    发明申请
    HIV-2 transmembrane glycoprotein homodimer (gp 80) 审中-公开
    HIV-2跨膜糖蛋白同源二聚体（gp 80）

    公开(公告)号：US20040010125A1

    公开(公告)日：2004-01-15

    申请号：US10422791

    申请日：2003-04-25

    申请人： INSTITUT PASTEUR

    发明人： Ara G. Hovanessian ,  Marie-Anne Rey ,  Anne G. Laurent ,  Bernard Krust ,  Luc Montagnier

    IPC分类号： C07K014/16 ,  A61K039/21

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K16/1063 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12N2740/16222 ,  Y10S435/974 ,  Y10S435/975 ,  Y10S530/826

    摘要： Characterization of the envelope transmembrane protein of human immunodeficiency virus type 2 (HIV-2) was carried out using murine polyclonal and monoclonal antibodies or patient sera specific for HIV-2 proteins. A 80-Mr glycoprotein (gp80) was produced in HIV-2 infected cells along with three other glycoproteins that were recently reported: the extracellular glycoprotein (gp125), the envelope glycoprotein precursor (gp140), and the transient dimeric form of gp140 (gp300). The gp125 and gp80 were detectable after the synthesis of gp140 and the formation of gp300. Among these four glycoproteins, only gp80 and gp125 were associated with HIV-2 virions. As the other glycoproteins, gp80 was recognized by all HIV-2 positive sera. A murine polyclonal antibody raised against the purified gp300 recognized all four glycoproteins. On the other hand, a monoclonal antibody raised against a synthetic polypeptide deduced from the sequence of the transmembrane glycoprotein of HIV-2, recognized gp140, gp300 and gp80; thus indicating that gp80 should be related to the transmembrane protein of the envelope. Dimerization of envelope glycoprotein precursor and the transmembrane glycoprotein was also observed in cells infected with simian immunodeficiency virus (SIV), a virus closely related to HIV-2. Dimerization of the envelope precursors might be essential for the processing of these glycoproteins into the mature products extracellular and transmembrane glycoproteins. Furthermore the dimeric form of the transmembrane glycoproteins might be important for the optimal structure of the virus and thus for its infectivity.

    摘要翻译： 使用鼠多克隆和单克隆抗体或HIV-2蛋白特异性的患者血清进行人类免疫缺陷病毒2型（HIV-2）的包膜跨膜蛋白的表征。 在HIV-2感染的细胞中产生80-Mr糖蛋白（gp80）以及最近报道的三种其他糖蛋白：细胞外糖蛋白（gp125），包膜糖蛋白前体（gp140）和gp140（gp140）的瞬时二聚体形式 ）。 在gp140的合成和gp300的形成之后，gp125和gp80是可检测的。 在这四种糖蛋白中，只有gp80和gp125与HIV-2病毒粒子相关。 作为其他糖蛋白，gp80被所有HIV-2阳性血清识别。 针对纯化的gp300产生的鼠多克隆抗体识别所有四种糖蛋白。 另一方面，从HIV-2的跨膜糖蛋白序列推断出的合成多肽产生的单克隆抗体，识别为gp140，gp300和gp80; 从而表明gp80应与信封的跨膜蛋白相关。 在与HIV-2密切相关的猿猴免疫缺陷病毒（SIV）感染的细胞中也观察到包膜糖蛋白前体和跨膜糖蛋白的二聚体。 包膜前体的二聚化可能对于将这些糖蛋白加工成细胞外和跨膜糖蛋白的成熟产物是至关重要的。 此外，跨膜糖蛋白的二聚形式对于病毒的最佳结构以及因此其感染性可能是重要的。

    Immunological reagents and diagnostic methods for the detection of human immunodeficiency virus type 2 utilizing multimeric forms of the envelope proteins GP300, P200, and P90/80
    10.
    发明申请
    Immunological reagents and diagnostic methods for the detection of human immunodeficiency virus type 2 utilizing multimeric forms of the envelope proteins GP300, P200, and P90/80 失效
    使用多聚体形式的包膜蛋白GP300，P200和P90 / 80检测人类免疫缺陷病毒2型的免疫学试剂和诊断方法

    公开(公告)号：US20010006641A1

    公开(公告)日：2001-07-05

    申请号：US09739289

    申请日：2000-12-19

    申请人： Institut Pasteur

    发明人： Luc Montagnier ,  Anne G. Laurent-Crawford ,  Bernard Krust ,  Ara G. Hovanessian ,  Marie-Anne Rey-Cuille

    IPC分类号： A61K039/21 ,  C12P021/08 ,  C07K016/00 ,  C12Q001/70

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  C07K16/1063 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222

    摘要： Four glycoproteins of apparent molecular weights 300,000, 140,000, 125,000, and 36,000 (gp300, gp140, gp125, and gp36) are detectable in human immunodeficiency virus type 2 (HIV-2) infected cells. The gp125 and gp36 are the external and transmembrane components, respectively, of the envelope glycoproteins of HIV-2 mature virions. The gp300, which is a dimeric form of gp14O, the precursor of HIV-2 envelope glycoprotein, is probably formed by a pH dependent fusion in the endoplasmic reticulum. Such a doublet is also observed in cells infected with simian immunodeficiency virus (SIV), a virus closely related to HIV-2. On the other hand, the envelope glycoprotein precursor of HIV-1 does not form a dimer during its processing. Experiments carried out with various inhibitors of oligosaccharide trimming enzymes suggest that transient dimerization of the glycoprotein precursor is required for its efficient transport to the Golgi apparatus and for its processing. The gp300 is useful for detecting antibodies to HIV-2 antigens in human body fluids and for raising antibodies to gp300.

    摘要翻译： 在人类免疫缺陷病毒2型（HIV-2）感染细胞中可检测到四种表观分子量为30万，14万，125,000和36,000（gp300，gp140，gp125和gp36）的糖蛋白。 gp125和gp36分别是HIV-2成熟病毒粒子的包膜糖蛋白的外部和跨膜组分。 作为HIV-2包膜糖蛋白的前体的gp14O的二聚体形式的gp300可能通过内质网中的pH依赖融合形成。 在感染了与HIV-2密切相关的猿猴免疫缺陷病毒（SIV）的细胞中也观察到这样的双峰。 另一方面，HIV-1的包膜糖蛋白前体在其加工过程中不形成二聚体。 用各种寡糖修饰酶抑制剂进行的实验表明，糖蛋白前体的瞬时二聚需要其有效转运到高尔基体并进行加工。 gp300可用于检测人体液中HIV-2抗原的抗体和提高gp300的抗体。