摘要:
Plants, particularly cereal plants, which have altered ability to synthesise starch are produced by inserting into the genome of the plant a gene encoding an enzyme of the starch biosynthetic pathway such that capacity to produce starch is improved or reduced. The gene encoding the enzyme ADP-glucose pyrophosphorylase has been isolated from wheat leaf and wheat endosperm tissue. The enzyme is essential to the biosynthesis of starch in plants. Addition of extra copies of the gene to a plant by transformation enhances starch production, increasing the food value of the crop. Conversely, insertion of a gene encoding mRNA antisense to the mRNA encoded by the endogenous ADP-glucose pyrophosphorylase gene will reduce starch production. cDNA sequences are provided which may be used to identify homologous sequences in other tissue and in other plants. The ADP-glucose pyrophosphorylase gene promoter may be used in the genetic manipulation of plants to alter their starch-synthesising ability.
摘要:
Plants with an altered starch synthesizing ability are produced by incorporating into the genome of the plant at least one donor gene encoding a starch primer. The starch primer is an enzyme capable of initiating starch synthesis, such as an amylogenin and/or glycogenin. DNA constructs encoding a starch primer are provided, particularly constructs encoding amylogenin from maize.
摘要:
Novel plants having an altered ability to produce starch, including an improved ability to produce structurally-altered starch or starch of altered quality are disclosed. The invention further relates to processes for obtaining such plants, wherein mutant plants are intercrossed to affect starch biosynthesis.
摘要:
The invention relates to a transgenic or mutated plant having genomic material which alters the normal starch synthesis pathway within the plant. More specifically, the present invention relates to a plant having a genotype which creates new forms of starch in significant quantity. Particularly, the invention relates to grain having an embryo with a genotype heterozygous for two or more wild type genes (for example, Aa/Bb) and an endosperm having a genotype heterozygous for such genes (for example, AAa/BBb or AAa/bbB or aaA/BBb or aaA/bbB) and the starch produced therefrom.
摘要:
A single controller interface (Smart-Stat) integrates the control of heating or cooling in buildings by simultaneously controlling Heating, Ventilation and Air Conditioning (HVAC) systems in concert with separate fresh air ventilation (FAV) systems. The Smart-Stat reduces costs and the carbon footprint of typical HVAC systems by optimizing the use of FAV. User-programmable set-points are incorporated with time-of-day and day-of-week as well as data from multiple sensors, thermostats and weather information. Mathematical algorithms are used to determine control signals to the HVAC or FAV systems. The Smart-Stat integrates the two separate systems into a single system that is able to direct the call for cooling or heating to the HVAC or FAV systems, depending on appropriate outside weather conditions. Any building can replace its existing HVAC system controller with the Smart-Stat controller and incorporate a FAV system to create a single integrated HVAC and FAV system.
摘要:
The present invention is related to the field of heating, ventilation and air conditioning (HVAC). More particularly, the present invention is related to methods and systems for controlled heating and cooling in order to reduce costs and the carbon footprint of said heating and cooling by optimizing the use of fresh air ventilation. The present invention is directed to mathematical algorithms incorporated into a controller and a method of determining control signals that are dependent on said mathematical algorithms and user programming that integrates information from multiple sensors, thermostats as well as weather information. Used in any home or building, the controller controls heating, cooling and ventilation systems in order to reduce costs and the carbon footprint of said heating and cooling by optimizing the use of fresh air ventilation. The controller works with typical HVAC systems generally in buildings and homes. The Smart-Stat algorithms are programmed into the controller and enable the controller to identify user-determined set-points alongside data from one or multiple internal temperature sensors. The user-determined set-points are also linked to time of day and day of week in a manner typical for typical thermostat devices available today. In such typical thermostat devices the controller will call for cooling or heating depending on the set points and conditions determined by the sensors in the building. The present invention is capable of interrupting the call for cooling or heating depending on whether the mathematical algorithms identify suitable outside weather conditions that permit the use of outside air cooling or outside air heating. Thus the call for heating or cooling can be redirected to call for ventilation instead of heating or cooling.
摘要:
The invention relates to a transgenic or mutated plant having genomic material which alters the normal starch synthesis pathway within the plant. More specifically, the present invention relates to a plant having a genotype which creates new forms of starch in significant quantity. Particularly, the invention relates to grain having an embryo with a genotype heterozygous for two or more wild type genes (for example, Aa/Bb) and an endosperm having a genotype heterozygous for such genes (for example, AAa/BBb or AAa/bbB or aaA/BBb or aaA/bbB) and the starch produced therefrom.
摘要:
A method of producing a plant with novel starch-synthesising ability comprises stably incorporating into the genome of a recipient plant at least one target gene encoding an enzyme involved in a starch or glycogen biosynthetic pathway. A plant with novel starch-synthesising ability may have novel starch quality (eg altered fine structure). Starch or glycogen biosynthetic enzymes include soluble starch synthase, branching enzyme, glycogen synthase, ADP-glucose pyrophosphorylase, self-glucosylating protein, glycogenin and amylogenin. Mutants involving said enzymes for use in this method are described, including specific gene-dosage combinations which provide novel starch fine structures and starch properties. DNA constructs for use in this method are described, as well as plants transformed with said DNA constructs, the seeds and progeny of such plants, and hybrids whose pedigree includes such plants.