公开(公告)号：US12227802B2

公开(公告)日：2025-02-18

申请号：US18488801

申请日：2023-10-17

Applicant: Illumina Cambridge Limited

Inventor： Antoine Francais ,  Elena Cressina ,  Adam Culley ,  Angelica Mariani ,  Xiaolin Wu ,  Xiaohai Liu

IPC: C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C12Q1/6869

Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3′ AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3′-OH blocking group for sequencing applications.

公开(公告)号：US11787831B2

公开(公告)日：2023-10-17

申请号：US17353512

申请日：2021-06-21

Applicant: Illumina Cambridge Limited

Inventor： Antoine Francais ,  Elena Cressina ,  Angelica Mariani ,  Adam Culley ,  Anno Koetje ,  Xiaohai Liu

IPC: C07H19/073 ,  C07H19/173 ,  C12Q1/6869

CPC classification number: C07H19/073 ,  C07H19/173 ,  C12Q1/6869

Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal 3′-OH blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3′ acetal blocking group for sequencing applications.

公开(公告)号：US20220396832A1

公开(公告)日：2022-12-15

申请号：US17748498

申请日：2022-05-19

Applicant: Illumina Cambridge Limited ,  Illumina, Inc.

Inventor： Angelica Mariani ,  Antoine Francais ,  Frederick James Topping ,  Christopher Winnard ,  Philip Balding ,  Chol Steven Yun ,  Patrizia Iavicoli

IPC: C12Q1/6869

Abstract: The present application relates to compositions and methods for sequencing by synthesis, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.

公开(公告)号：US11827931B2

公开(公告)日：2023-11-28

申请号：US17708498

申请日：2022-03-30

Applicant: Illumina Cambridge Limited

Inventor： Antoine Francais ,  Elena Cressina ,  Adam Culley ,  Angelica Mariani ,  Xiaolin Wu ,  Xiaohai Liu

IPC: C12Q1/68 ,  C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00

CPC classification number: C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00

Abstract: Embodiments of the present disclosure relate to methods of preparing growing polynucleotides using nucleotide molecules with a 3′ AOM blocking group. Also provided herein are kits related to such methods.

公开(公告)号：US11293061B2

公开(公告)日：2022-04-05

申请号：US16724088

申请日：2019-12-20

Applicant: Illumina Cambridge Limited

Inventor： Antoine Francais ,  Elena Cressina ,  Adam Culley ,  Angelica Mariani ,  Xiaolin Wu ,  Xiaohai Liu

IPC: C12Q1/68 ,  C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00

Abstract: Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3′ AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.