公开(公告)号：US20240352048A1

公开(公告)日：2024-10-24

申请号：US18760347

申请日：2024-07-01

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Michael Callingham ,  Nikolai Nikolaevich Romanov ,  Xiaohai Liu ,  Justyna Piekos

IPC分类号： C07F5/02 ,  C07H23/00

CPC分类号： C07F5/027 ,  C07H23/00

摘要： The present application relates to substituted dyes containing bis-boron fused heterocycles and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

公开(公告)号：US12071662B2

公开(公告)日：2024-08-27

申请号：US18313227

申请日：2023-05-05

申请人： Illumina Cambridge Limited

发明人： Elena Cressina ,  Antoine Francais ,  Xiaohai Liu

IPC分类号： C12Q1/68 ,  C07H19/06 ,  C07H19/073 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6869

CPC分类号： C12Q1/6869 ,  C07H19/06 ,  C07H19/073 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2535/122

摘要： The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.

公开(公告)号：US11827931B2

公开(公告)日：2023-11-28

申请号：US17708498

申请日：2022-03-30

申请人： Illumina Cambridge Limited

发明人： Antoine Francais ,  Elena Cressina ,  Adam Culley ,  Angelica Mariani ,  Xiaolin Wu ,  Xiaohai Liu

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00

CPC分类号： C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00

摘要： Embodiments of the present disclosure relate to methods of preparing growing polynucleotides using nucleotide molecules with a 3′ AOM blocking group. Also provided herein are kits related to such methods.

公开(公告)号：US20220145379A1

公开(公告)日：2022-05-12

申请号：US17599994

申请日：2020-11-25

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Wayne N. George ,  Brian D. Mather ,  Andrew A. Brown ,  Pietro Gatti Lafranconi ,  Maria Candelaria Rogert Bacigalupo ,  Antoine Francais ,  Xiaohai Liu

IPC分类号： C12Q1/6848 ,  C12Q1/6869

摘要： An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3′ OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.

公开(公告)号：US11293061B2

公开(公告)日：2022-04-05

申请号：US16724088

申请日：2019-12-20

申请人： Illumina Cambridge Limited

发明人： Antoine Francais ,  Elena Cressina ,  Adam Culley ,  Angelica Mariani ,  Xiaolin Wu ,  Xiaohai Liu

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00

摘要： Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3′ AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.

公开(公告)号：US11028116B2

公开(公告)日：2021-06-08

申请号：US17035053

申请日：2020-09-28

申请人： Illumina Cambridge Limited

发明人： Xiaohai Liu ,  John Milton ,  Silke Ruediger ,  Xiaolin Wu

IPC分类号： C12Q1/68 ,  C07H19/04 ,  C07H19/16 ,  C07H19/20 ,  C07H19/14 ,  C07H19/06 ,  C07H21/00 ,  C07H19/10 ,  C12Q1/6869 ,  C07H21/04 ,  C12Q1/6874

摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).

公开(公告)号：US11008359B2

公开(公告)日：2021-05-18

申请号：US16916887

申请日：2020-06-30

申请人： Illumina Cambridge Limited

发明人： Xiaohai Liu ,  John Milton ,  Silke Ruediger ,  Xiaolin Wu

IPC分类号： C07H19/04 ,  C07H19/06 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/68

摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).

公开(公告)号：US10480025B2

公开(公告)日：2019-11-19

申请号：US15202456

申请日：2016-07-05

申请人： Illumina Cambridge Limited

发明人： Shankar Balasubramanian ,  Colin Lloyd Barnes ,  Xiaohai Liu ,  John Milton ,  Xiaolin Wu ,  Harold Swerdlow

IPC分类号： C07H21/00 ,  C12Q1/6869 ,  C07H19/10 ,  C07H19/20 ,  C12P19/34 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/6818 ,  C12Q1/6834 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/68

摘要： Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.

公开(公告)号：US20190153529A1

公开(公告)日：2019-05-23

申请号：US16258401

申请日：2019-01-25

申请人： Illumina Cambridge Limited

发明人： Xiaolin Wu ,  Xiaohai Liu

IPC分类号： C12Q1/6869 ,  C07H19/14

摘要： Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.

公开(公告)号：US20180353926A1

公开(公告)日：2018-12-13

申请号：US16010902

申请日：2018-06-18

申请人： Illumina Cambridge Limited

发明人： Xiaohai Liu ,  John Milton ,  Geoffrey Paul Smith ,  Colin Lloyd Barnes ,  Isabelle Rasolonjatovo ,  Roberto Rigatti ,  Xiaolin Wu ,  Tobias William Barr Ost ,  Graham John Worsley ,  David James Earnshaw ,  Gerardo Turcatti ,  Anthony Romieu

IPC分类号： B01J19/00 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

CPC分类号： B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00675 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2535/101 ,  C12Q2523/113 ,  C12Q2523/107 ,  C12Q2521/331 ,  C12Q2521/307

摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.