公开(公告)号：US20220396832A1

公开(公告)日：2022-12-15

申请号：US17748498

申请日：2022-05-19

Applicant: Illumina Cambridge Limited ,  Illumina, Inc.

Inventor： Angelica Mariani ,  Antoine Francais ,  Frederick James Topping ,  Christopher Winnard ,  Philip Balding ,  Chol Steven Yun ,  Patrizia Iavicoli

IPC: C12Q1/6869

Abstract: The present application relates to compositions and methods for sequencing by synthesis, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.

公开(公告)号：US20220145379A1

公开(公告)日：2022-05-12

申请号：US17599994

申请日：2020-11-25

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Brian D. Mather ,  Andrew A. Brown ,  Pietro Gatti Lafranconi ,  Maria Candelaria Rogert Bacigalupo ,  Antoine Francais ,  Xiaohai Liu

IPC: C12Q1/6848 ,  C12Q1/6869

Abstract: An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3′ OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.

公开(公告)号：US12227802B2

公开(公告)日：2025-02-18

申请号：US18488801

申请日：2023-10-17

Applicant: Illumina Cambridge Limited

Inventor： Antoine Francais ,  Elena Cressina ,  Adam Culley ,  Angelica Mariani ,  Xiaolin Wu ,  Xiaohai Liu

IPC: C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C12Q1/6869

Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3′ AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3′-OH blocking group for sequencing applications.

公开(公告)号：US20210198724A1

公开(公告)日：2021-07-01

申请号：US17191390

申请日：2021-03-03

Applicant: ILLUMINA CAMBRIDGE LIMITED

Inventor： Antoine Francais ,  Xiaohai Liu

IPC: C12Q1/6816 ,  C09B23/06 ,  C07D209/08 ,  C07D403/06 ,  C09B23/08 ,  G01N33/58 ,  C09B23/10

Abstract: The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

公开(公告)号：US10982261B2

公开(公告)日：2021-04-20

申请号：US15762073

申请日：2016-09-26

Applicant: ILLUMINA CAMBRIDGE LIMITED

Inventor： Antoine Francais ,  Xiaohai Liu

IPC: C12Q1/68 ,  C09B23/06 ,  C12Q1/6816 ,  C07D209/08 ,  C07D403/06 ,  C09B23/08 ,  G01N33/58 ,  C09B23/10

Abstract: The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

公开(公告)号：US20230348968A1

公开(公告)日：2023-11-02

申请号：US18313227

申请日：2023-05-05

Applicant: Illumina Cambridge Limited

Inventor： Elena Cressina ,  Antoine Francais ,  Xiaohai Liu

IPC: C12Q1/6869 ,  C07H19/06 ,  C07H21/00 ,  C07H19/16 ,  C07H19/20 ,  C07H19/10 ,  C07H19/073 ,  C07H19/14 ,  C07H21/04 ,  C12Q1/6809 ,  C12Q1/6816

CPC classification number: C12Q1/6869 ,  C07H19/06 ,  C07H21/00 ,  C07H19/16 ,  C07H19/20 ,  C07H19/10 ,  C07H19/073 ,  C07H19/14 ,  C07H21/04 ,  C12Q1/6809 ,  C12Q1/6816

Abstract: The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.

公开(公告)号：US20230167486A1

公开(公告)日：2023-06-01

申请号：US18062232

申请日：2022-12-06

Applicant: ILLUMINA CAMBRIDGE LIMITED

Inventor： Antoine Francais ,  Xiaohai Liu

IPC: C12Q1/6816 ,  C09B23/06 ,  C07D209/08 ,  C07D403/06 ,  C09B23/08 ,  G01N33/58 ,  C09B23/10

CPC classification number: C12Q1/6816 ,  C07D209/08 ,  C07D403/06 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  G01N33/582

Abstract: The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

公开(公告)号：US11597969B2

公开(公告)日：2023-03-07

申请号：US17103169

申请日：2020-11-24

Applicant: Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Xiaohai Liu ,  Nikolai Nikolaevich Romanov ,  Antoine Francais ,  Patrick McCauley ,  Michael Callingham ,  Carole Anastasi

IPC: C12Q1/68 ,  C09B57/02 ,  C12Q1/6869

Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.

公开(公告)号：US11981955B2

公开(公告)日：2024-05-14

申请号：US18062232

申请日：2022-12-06

Applicant: ILLUMINA CAMBRIDGE LIMITED

Inventor： Antoine Francais ,  Xiaohai Liu

IPC: C12Q1/68 ,  C07D209/08 ,  C07D403/06 ,  C09B23/06 ,  C09B23/08 ,  C09B23/10 ,  C12Q1/6816 ,  G01N33/58

CPC classification number: C12Q1/6816 ,  C07D209/08 ,  C07D403/06 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  G01N33/582 ,  C12Q1/6816 ,  C12Q2563/107 ,  C12Q2565/102

Abstract: The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

公开(公告)号：US11787831B2

公开(公告)日：2023-10-17

申请号：US17353512

申请日：2021-06-21

Applicant: Illumina Cambridge Limited

Inventor： Antoine Francais ,  Elena Cressina ,  Angelica Mariani ,  Adam Culley ,  Anno Koetje ,  Xiaohai Liu

IPC: C07H19/073 ,  C07H19/173 ,  C12Q1/6869

CPC classification number: C07H19/073 ,  C07H19/173 ,  C12Q1/6869

Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal 3′-OH blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3′ acetal blocking group for sequencing applications.