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    PYROPHOSPHOROLYTIC SEQUENCING
    1.
    发明申请
    PYROPHOSPHOROLYTIC SEQUENCING 审中-公开

    公开(公告)号：US20200157619A1

    公开(公告)日：2020-05-21

    申请号：US16657931

    申请日：2019-10-18

    申请人： Illumina Cambridge Limited

    发明人： Wouter Meuleman

    IPC分类号： C12Q1/6869

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.

    Pyrophosphorolytic sequencing
    2.
    发明授权
    Pyrophosphorolytic sequencing 有权

    公开(公告)号：US10450605B2

    公开(公告)日：2019-10-22

    申请号：US15847362

    申请日：2017-12-19

    申请人： Illumina Cambridge Limited

    发明人： Wouter Meuleman

    IPC分类号： C12P19/34 ,  C12Q1/6869

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.

    Pyrophosphorolytic sequencing
    3.
    发明授权
    Pyrophosphorolytic sequencing 有权

    公开(公告)号：US11286521B2

    公开(公告)日：2022-03-29

    申请号：US16657931

    申请日：2019-10-18

    申请人： Illumina Cambridge Limited

    发明人： Wouter Meuleman

    IPC分类号： C12Q1/6869

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.

    PYROPHOSPHOROLYTIC SEQUENCING
    7.
    发明申请
    PYROPHOSPHOROLYTIC SEQUENCING 审中-公开

    公开(公告)号：US20180105874A1

    公开(公告)日：2018-04-19

    申请号：US15847362

    申请日：2017-12-19

    申请人： Illumina Cambridge Limited

    发明人： Wouter Meuleman

    IPC分类号： C12Q1/6869

    CPC分类号： C12Q1/6869 ,  C12Q2521/319 ,  C12Q2521/525 ,  C12Q2565/631

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.

    PYROPHOSPHOROLYTIC SEQUENCING
    8.
    发明申请
    PYROPHOSPHOROLYTIC SEQUENCING 有权

    公开(公告)号：US20220177960A1

    公开(公告)日：2022-06-09

    申请号：US17677844

    申请日：2022-02-22

    申请人： Illumina Cambridge Limited

    发明人： Wouter Meuleman

    IPC分类号： C12Q1/6869

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.

    Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
    9.
    发明授权
    Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing 有权

    公开(公告)号：US10378051B2

    公开(公告)日：2019-08-13

    申请号：US13627134

    申请日：2012-09-26

    申请人： ILLUMINA CAMBRIDGE LIMITED

    发明人： Wouter Meuleman ,  Elena Cressina ,  Geoffrey Paul Smith ,  Rosamond Jackson ,  Xiaohai Liu

    IPC分类号： C40B20/00 ,  C12Q1/68 ,  C12Q1/6874

    摘要： A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.

    CONTINUOUS EXTENSION AND DEBLOCKING IN REACTIONS FOR NUCLLEIC ACIDS SYNTHESIS AND SEQUENCING
    10.
    发明申请
    CONTINUOUS EXTENSION AND DEBLOCKING IN REACTIONS FOR NUCLLEIC ACIDS SYNTHESIS AND SEQUENCING 审中-公开
    连续延伸和解除核酸合成和测序的反应

    公开(公告)号：US20130085073A1

    公开(公告)日：2013-04-04

    申请号：US13627134

    申请日：2012-09-26

    申请人： Illumina Cambridge Limited

    发明人： Wouter Meuleman ,  Elena Cressina ,  Geoffrey Paul Smith ,  Rosamond Jackson ,  Xiaohai Liu

    IPC分类号： C40B20/00 ,  C12Q1/68 ,  G01N21/64 ,  C12P19/34

    CPC分类号： C12Q1/6874 ,  C12Q2521/101 ,  C12Q2523/107 ,  C12Q2525/101 ,  C12Q2525/186 ,  C12Q2565/1015

    摘要： A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.

    摘要翻译： 一种反应混合物，其包含（a）具有与模板杂交的引物的核酸，（b）核苷酸类似物，其中所述核苷酸类似物具有阻断部分; （c）通过向引物中加入核苷酸类似物，能够形成延伸引物的聚合酶，和（d）能够从延伸引物除去阻断部分的解封剂。 还提供了合成多核苷酸的方法，所述多核苷酸包括在反应混合物中通过几个反应循环顺序加入多个不同的核苷酸类似物，其中每个反应循环包括（i）将核苷酸类似物加入到核酸中 以形成包含阻断部分的瞬时核酸物质，和（ii）修饰瞬态核酸物质以去除阻断部分的解封端剂。