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公开(公告)号:US20060223186A1
公开(公告)日:2006-10-05
申请号:US11369497
申请日:2006-03-07
申请人: Igor Goryshin , Todd Naumann , William Reznikoff
发明人: Igor Goryshin , Todd Naumann , William Reznikoff
摘要: Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.
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公开(公告)号:US20190040382A1
公开(公告)日:2019-02-07
申请号:US15519482
申请日:2015-10-16
申请人: Frank J. STEEMERS , Kevin L. GUNDERSON , Fan ZHANG , Jason Richard BETLEY , Niall Anthony GORMLEY , Wouter MEULEMAN , Jacqueline WEIR , Avgousta IOANNOU , Gareth JENKINS , Rosamond JACKSON , Natalie MORRELL , Dmitry K. POKHOLOK , Steven J. NORBERG , Molly HE , Amirali KIA , Igor GORYSHIN , Rigo PANTOJA , ILLUMINA CAMBRIDGE LIMITED
发明人: Frank J. Steemers , Kevin L. Gunderson , Fan Zhang , Jason Richard Betley , Niall Anthony Gormley , Wouter Meuleman , Jacqueline Weir , Avgousta Ioannou , Gareth Jenkins , Rosamond Jackson , Natalie Morrell , Dmitry K. Pokholok , Steven J. Norberg , Molly He , Amirali Kia , Igor Goryshin , Rigo Pantoja
摘要: Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.
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3.
公开(公告)号:US09238671B2
公开(公告)日:2016-01-19
申请号:US13979346
申请日:2012-01-30
申请人: Igor Goryshin , Bradley Baas , Ramesh Vaidyanathan , Mark Maffitt
发明人: Igor Goryshin , Bradley Baas , Ramesh Vaidyanathan , Mark Maffitt
CPC分类号: C12N15/1065 , C07H21/00 , C12N9/1241 , C12N15/1093 , C12P19/34 , C12Q1/6855 , C40B50/06
摘要: Transposomes and oligonucleotide replacement methods to make DNA libraries that have distinct 5′ and 3′ tags, and to make directional libraries that are enriched for a desired strand.
摘要翻译: 用于制备具有不同5'和3'标签的DNA文库的转座子和寡核苷酸替代方法,并制备富集所需链的定向文库。
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