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    Methods for indexing samples and sequencing multiple polynucleotide templates
    2.
    发明授权
    Methods for indexing samples and sequencing multiple polynucleotide templates 有权
    索引样本和测序多个多核苷酸模板的方法

    公开(公告)号：US08822150B2

    公开(公告)日：2014-09-02

    申请号：US13444136

    申请日：2012-04-11

    申请人： Helen Bignell ,  Louise Fraser ,  Niall Anthony Gormley

    发明人： Helen Bignell ,  Louise Fraser ,  Niall Anthony Gormley

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6874 ,  C12Q1/6837 ,  C12Q1/6855 ,  C12Q2537/143 ,  C12Q2525/161

    摘要： The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.

    摘要翻译： 本发明涉及在多核苷酸模板的测序期间对样品进行索引的方法，导致特异于每个核酸样品的来源的标签的附着，使得在测序运行之后，可以确定每个多核苷酸的来源和序列。 因此，本发明涉及复杂基因组（例如，人类基因组）的分析，以及复合较不复杂的基因组，例如细菌，病毒，线粒体等的分析。

    Preparation of nucleic acid templates for solid phase amplification
    3.
    发明授权
    Preparation of nucleic acid templates for solid phase amplification 有权
    制备用于固相扩增的核酸模板

    公开(公告)号：US08168388B2

    公开(公告)日：2012-05-01

    申请号：US12085508

    申请日：2006-11-24

    申请人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerardo Turcatti ,  Colin Lloyd Barnes

    发明人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerardo Turcatti ,  Colin Lloyd Barnes

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6855 ,  C12Q2565/537

    摘要： The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

    摘要翻译： 本发明涉及制备用于固相核酸扩增的模板构建体的方法以及在固相核酸扩增方法中使用该模板的方法。 该方法包括进行两个连接反应：（a）连接反应，其中一个或多个靶多核苷酸分子的第一末端连接到表面结合的衔接子多核苷酸分子，和（b）连接反应，其中溶液相适配器 将多核苷酸分子连接到所述靶多核苷酸分子的第二末端，以便产生连接到固体支持物上的一个或多个模板构建体。

    End Modification to Prevent Over-Representation of Fragments
    6.
    发明申请
    End Modification to Prevent Over-Representation of Fragments 有权
    最终修改以防止片段过度表示

    公开(公告)号：US20090176662A1

    公开(公告)日：2009-07-09

    申请号：US12223761

    申请日：2007-02-07

    申请人： Roberto Rigatti ,  Niall Anthony Gormley ,  Helen Rachel Bignell

    发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Helen Rachel Bignell

    IPC分类号： C40B40/08 ,  C40B50/06

    CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12Q1/6855 ,  C12Q2525/119 ,  C12Q2525/186

    摘要： The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from whence the 5′ and 3′ modified library is generated is greatly reduced or prevented.

    摘要翻译： 本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，以及在固相核酸扩增方法中使用5'和3'修饰的模板文库。 特别地，本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，该文库在其5'末端和3'末端具有共同序列，其中主要“末端”序列的过表达 产生5'和3'修饰的文库的多核苷酸分子大大降低或防止。

    Method of target enrichment
    7.
    发明申请
    Method of target enrichment 审中-公开
    目标浓缩方法

    公开(公告)号：US20080274904A1

    公开(公告)日：2008-11-06

    申请号：US11891290

    申请日：2007-08-09

    申请人： Niall Anthony Gormley ,  John Stephen West

    发明人： Niall Anthony Gormley ,  John Stephen West

    IPC分类号： C40B10/00

    CPC分类号： C12Q1/6869 ,  C12Q2565/501 ,  C12Q2525/191

    摘要： The present invention is directed to a method for reducing the complexity of a nucleic acid sample in a reproducible manner by enriching for specific nucleic acid target sequences in the population of nucleic acids. More specifically, the invention relates to a method for enriching specific target sequences in a population using libraries of oligonucleotides.

    摘要翻译： 本发明涉及通过富集核酸群体中的特定核酸靶序列，以可再现的方式降低核酸样品的复杂性的方法。 更具体地，本发明涉及使用寡核苷酸文库丰富群体中特异性靶序列的方法。

    Methods for sequencing polynucleotides
    8.
    发明授权
    Methods for sequencing polynucleotides 有权
    多核苷酸测序方法

    公开(公告)号：US09029103B2

    公开(公告)日：2015-05-12

    申请号：US13219422

    申请日：2011-08-26

    申请人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

    发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12Q1/6874 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2565/513 ,  C12Q2565/543

    摘要： Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.

    摘要翻译： 本文提供了用于测序多核苷酸分子的方法。 该方法包括提供连接到表面的多个多核苷酸分子的步骤，其中每个多核苷酸分子的第一部分连接到该表面的第一位置，并且每个多核苷酸分子的第二部分连接到该多核苷酸分子的第二位置 表面，第一和第二位置的相对接近度与第一和第二部分配对的概率相关，分离表面上的多核苷酸分子的第一和第二部分，确定第一和第二部分的序列 多核苷酸分子，并比较相对接近度和序列以确定哪个第一和第二部分配对并确定靶多核苷酸分子的序列。