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公开(公告)号：US20070141064A1
公开(公告)日：2007-06-21
申请号：US11510490
申请日：2006-08-25
申请人： Ilya Trakht , Gavreel Kalantarov
发明人： Ilya Trakht , Gavreel Kalantarov
CPC分类号： C12N5/163 , C07K16/00 , C07K2317/21
摘要： Certain aspects of the present invention are directed to new fully human fusion partner cell lines called human Karyochi cells, and to methods for making them. Human Karyochi cells are then fused with human antibody-secreting lymphoid cells to make fully human hybridomas called Karyochi-based hybridomas, which likewise secrete fully human monoclonal antibodies. Human Karyochi cells are made by isolating a donor nucleus that is substantially free of cytoplasm from either a first malignant B-lymphocyte cell line or a normal B-lymphocyte, and transferring the donor nucleus into the cytoplasm of a recipient cell from a second T- or B-lymphoid cell line. With time the nuclei synchronize and fuse to form the chimeric Karyochi fusion partner cell line. Nuclear transfer can be accomplished using intra-cytosolic nucleus injection or by impact-induced nucleus administration.
摘要翻译： 本发明的某些方面涉及称为人类Karyochi细胞的新的完全人融合伙伴细胞系，以及制备它们的方法。 然后将人类Karyochi细胞与人抗体分泌淋巴样细胞融合以制备完全人类杂交瘤，称为基于核型的杂交瘤，其同样分泌完全的人单克隆抗体。 通过从第一恶性B淋巴细胞细胞系或正常B淋巴细胞中分离基本上不含细胞质的供体核，并将供体核从第二T细胞转移到受体细胞的细胞质中制备人类核型细胞， 或B淋巴细胞系。 随着时间的推移，核同步并融合形成嵌合的Karyochi融合伙伴细胞系。 核转移可以使用胞内核注射或通过冲击诱导的核给药来完成。
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公开(公告)号：US08859278B2
公开(公告)日：2014-10-14
申请号：US11510490
申请日：2006-08-25
申请人： Ilya Trakht , Gavreel Kalantarov
发明人： Ilya Trakht , Gavreel Kalantarov
IPC分类号： C12N5/0781 , C12N5/09 , C12N5/10 , C07K16/00 , C12N5/16
CPC分类号： C12N5/163 , C07K16/00 , C07K2317/21
摘要： Certain aspects of the present invention are directed to new fully human fusion partner cell lines called human Karyochi cells, and to methods for making them. Human Karyochi cells are then fused with human antibody-secreting lymphoid cells to make fully human hybridomas called Karyochi-based hybridomas, which likewise secrete fully human monoclonal antibodies. Human Karyochi cells are made by isolating a donor nucleus that is substantially free of cytoplasm from either a first malignant B-lymphocyte cell line or a normal B-lymphocyte, and transferring the donor nucleus into the cytoplasm of a recipient cell from a second T- or B-lymphoid cell line. With time the nuclei synchronize and fuse to form the chimeric Karyochi fusion partner cell line. Nuclear transfer can be accomplished using intra-cytosolic nucleus injection or by impact-induced nucleus administration.
摘要翻译： 本发明的某些方面涉及称为人类Karyochi细胞的新的完全人融合伙伴细胞系，以及制备它们的方法。 然后将人类Karyochi细胞与人抗体分泌淋巴样细胞融合以制备完全人类杂交瘤，称为基于核型的杂交瘤，其同样分泌完全的人单克隆抗体。 通过从第一恶性B淋巴细胞细胞系或正常B淋巴细胞中分离基本上不含细胞质的供体核，并将供体核从第二T细胞转移到受体细胞的细胞质中制备人类核型细胞， 或B淋巴细胞系。 随着时间的推移，核同步并融合形成嵌合的Karyochi融合伙伴细胞系。 核转移可以使用胞内核注射或通过冲击诱导的核给药来完成。
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公开(公告)号：US20100323907A1
公开(公告)日：2010-12-23
申请号：US12446575
申请日：2006-10-27
CPC分类号： A01N1/0231 , A01N1/0221
摘要： The invention is directed to a new composition for making a housing block for cryosectioning comprising agarose and optimal cutting temperature medium. The invention is further directed to new methods for making a frozen section microarray of fresh non-fixed frozen cell or tissue samples that undergo only one freeze-thaw cycle before being used in a biological assay.
摘要翻译： 本发明涉及用于制备用于冷冻切片的壳体块的新组合物，其包括琼脂糖和最佳切割温度培养基。 本发明进一步涉及用于制造新鲜未固定的冷冻细胞或组织样品的冷冻切片微阵列的新方法，其仅在生物测定中使用之前仅经历一个冻融循环。
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公开(公告)号：US07820400B2
公开(公告)日：2010-10-26
申请号：US11453186
申请日：2006-06-13
IPC分类号： G01N33/574
CPC分类号： G01N33/57407 , C07K16/28 , C07K16/3015 , C07K2317/21 , C07K2317/77 , C12N5/163 , C12N5/166 , C12N2510/02 , C12N2510/04 , G01N33/57492
摘要： The present invention provides monoclonal antibody-producing hybridomas designated 27.F7 and 27.B1. The invention provides a method of detecting TIP-2 antigen bearing cancer cells in a sample. The invention provides a method of detecting TIP-2 antigen on the surface of cancer cells. The invention provides a method for diagnosing cancer in a subject. The invention provides a method for delivering exogenous material to TIP-2 antigen-bearing cancer cells of a human subject. The invention provides a method for treating cancer in a human subject. The invention provides isolated peptides having the amino acid sequences Lys Leu Leu Gly Gly Gln Ile Gly Leu (SEQ. ID No.) and Ser Leu Leu Gly Cys Arg His Tyr Glu Val (SEQ. ID No.). The invention provides a method for immunohistochemical screening of a tissue section for the presence of TIP-2 antigen bearing cancer cells. The invention provides a kit for detecting the presence of TIP-2 antigen-bearing cancer cells. The invention provides a method for detecting the presence of TIP-2 antigen. The invention provides a method for immunohistochemical screening of tissue sections. The invention provides a method for monitoring progression of cancer wherein the cancer cells are TIP-2 antigen-bearing cells. The invention provides a method for diagnosing cancer associated with the expression of TIP-2.
摘要翻译： 本发明提供了称为27.F7和27.B1的产生单克隆抗体的杂交瘤。 本发明提供一种检测样品中携带TIP-2抗原的癌细胞的方法。 本发明提供了检测癌细胞表面的TIP-2抗原的方法。 本发明提供了一种用于诊断受试者的癌症的方法。 本发明提供了将外源性材料递送至人受试者的TIP-2抗原携带癌细胞的方法。 本发明提供了一种治疗人类受试者的癌症的方法。 本发明提供具有氨基酸序列Lys Leu Leu Gly Gly Gln Ile Gly Leu（SEQ ID NO）和Ser Leu Leu Gly Cys Arg His Tyr Glu Val的分离肽（SEQ ID NO：SEQ ID NO： 本发明提供了一种组织切片对于携带TIP-2抗原的癌细胞存在的免疫组织化学筛选方法。 本发明提供了用于检测携带TIP-2抗原的癌细胞的存在的试剂盒。 本发明提供了检测TIP-2抗原存在的方法。 本发明提供了组织切片的免疫组织化学筛选方法。 本发明提供了一种监测癌症进展的方法，其中癌细胞是携带TIP-2抗原的细胞。 本发明提供了一种诊断与TIP-2表达相关的癌症的方法。
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公开(公告)号：US20060292644A1
公开(公告)日：2006-12-28
申请号：US11453186
申请日：2006-06-13
IPC分类号： G01N33/574 , C12N5/06 , C07K16/30
CPC分类号： G01N33/57407 , C07K16/28 , C07K16/3015 , C07K2317/21 , C07K2317/77 , C12N5/163 , C12N5/166 , C12N2510/02 , C12N2510/04 , G01N33/57492
摘要： The present invention provides monoclonal antibody-producing hybridomas designated 27.F7 and 27.B1. The invention provides a method of detecting TIP-2 antigen bearing cancer cells in a sample. The invention provides a method of detecting TIP-2 antigen on the surface of cancer cells. The invention provides a method for diagnosing cancer in a subject. The invention provides a method for delivering exogenous material to TIP-2 antigen-bearing cancer cells of a human subject. The invention provides a method for treating cancer in a human subject. The invention provides isolated peptides having the amino acid sequences Lys Leu Leu Gly Gly Gln Ile Gly Leu (SEQ. ID No.) and Ser Leu Leu Gly Cys Arg His Tyr Glu Val (SEQ. ID No.). The invention provides a method for immunohistochemical screening of a tissue section for the presence of TIP-2 antigen bearing cancer cells. The invention provides a kit for detecting the presence of TIP-2 antigen-bearing cancer cells. The invention provides a method for detecting the presence of TIP-2 antigen. The invention provides a method for immunohistochemical screening of tissue sections. The invention provides a method for monitoring progression of cancer wherein the cancer cells are TIP-2 antigen-bearing cells. The invention provides a method for diagnosing cancer associated with the expression of TIP-2.
摘要翻译： 本发明提供了称为27.F7和27.B1的产生单克隆抗体的杂交瘤。 本发明提供一种检测样品中携带TIP-2抗原的癌细胞的方法。 本发明提供了检测癌细胞表面的TIP-2抗原的方法。 本发明提供了一种用于诊断受试者的癌症的方法。 本发明提供了将外源性材料递送至人受试者的TIP-2抗原携带癌细胞的方法。 本发明提供了一种治疗人类受试者的癌症的方法。 本发明提供具有氨基酸序列Lys Leu Leu Gly Gly Gln Ile Gly Leu（SEQ ID NO）和Ser Leu Leu Gly Cys Arg His Tyr Glu Val的分离肽（SEQ ID NO：SEQ ID NO： 本发明提供了一种组织切片对于携带TIP-2抗原的癌细胞存在的免疫组织化学筛选方法。 本发明提供了用于检测携带TIP-2抗原的癌细胞的存在的试剂盒。 本发明提供了检测TIP-2抗原存在的方法。 本发明提供了组织切片的免疫组织化学筛选方法。 本发明提供了一种监测癌症进展的方法，其中癌细胞是携带TIP-2抗原的细胞。 本发明提供了一种诊断与TIP-2表达相关的癌症的方法。
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公开(公告)号：US07060802B1
公开(公告)日：2006-06-13
申请号：US09664958
申请日：2000-09-18
IPC分类号： C07K16/00
CPC分类号： G01N33/57407 , C07K16/28 , C07K16/3015 , C07K2317/21 , C07K2317/77 , C12N5/163 , C12N5/166 , C12N2510/02 , C12N2510/04 , G01N33/57492
摘要： This invention provides monoclonal antibody-producing hybridomas designated 27.F7 and 27.B1. The invention also provides methods for detecting TIP-2 antigen-bearing cancer cells in a sample, detecting the presence of TIP-2 antigen, optionally on the surface of cancer cells, immunohistochemical screening of a tissue section for the presence of TIP-2 antigen bearing cancer cells, diagnosing cancer in a subject, monitoring progression of cancer wherein the cancer cells are TIP-2 antigen-bearing cells, delivering exogenous material to TIP-2 antigen-bearing cancer cells of a human subject, and treating cancer in a human subject. This invention further provides a kit for detecting the presence of TIP-2 antigen-bearing cancer cells. This invention also provides isolated peptides having the amino acid sequences Lys Leu Leu Gly Gly Gln Ile Gly Leu (SEQ ID No:3) and Ser Leu Leu Gly Cys Arg His Tyr Glu Val (SEQ ID NO:4).
摘要翻译： 本发明提供了称为27.F7和27.B1的产生单克隆抗体的杂交瘤。 本发明还提供了用于检测样品中TIP-2抗原的癌细胞的方法，任选地在癌细胞表面检测TIP-2抗原的存在，组织切片的TIP-2抗原的存在的免疫组织化学筛选 诊断受试者中的癌症，监测癌症的进展，其中癌细胞是TIP-2抗原携带细胞，将外源性物质递送到人受试者的TIP-2抗原携带癌细胞，以及治疗人类的癌症 学科。 本发明还提供了用于检测携带TIP-2抗原的癌细胞的存在的试剂盒。 本发明还提供了具有氨基酸序列Lys Leu Leu Gly Gly Gln Ile Gly Leu（SEQ ID No.3）和Ser Leu Leu Gly Cys Arg His Tyr Glu Val（SEQ ID NO：4）的分离肽。
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公开(公告)号：US06197582B1
公开(公告)日：2001-03-06
申请号：US09040833
申请日：1998-03-18
申请人： Ilya Trakht
发明人： Ilya Trakht
IPC分类号： C12N506
CPC分类号： C07K16/3069 , C07K16/1235 , C07K16/2863 , C07K16/3015 , C07K2317/21 , C12N5/163 , C12N5/166 , C12N2510/02 , C12N2510/04 , G01N33/56983 , G01N33/57484 , G01N33/6893 , Y10S530/808 , Y10S530/809
摘要： The present invention provides a trioma cell which does not produce any antibody obtained by fusing a hetermomyeloma cell which does not produce any antibody with a human lymphoid cell, wherein the heteromyeloma cell is designated B6B11. The invention also provides a tetroma cell capable of producing a monoclonal antibody having specific binding affinity for an antigen obtained by fusing a trioma cell which does not produce any antibody with a human lymphoid cell capable of producing antibody having specific binding affinity for the antigen. The invention also provides methods for generating trioma cells and tetroma cells, and the cells generated by the methods.
摘要翻译： 本发明提供不产生通过将不产生任何抗体的异源骨髓瘤细胞与人淋巴样细胞融合获得的任何抗体的三瘤细胞，其中所述异源骨髓瘤细胞被命名为B6B11。 本发明还提供能够产生对通过将不产生任何抗体的三瘤细胞与能够产生对抗原具有特异性结合亲和力的抗体的人淋巴样细胞融合而获得的抗原具有特异性结合亲和力的四联体细胞。 本发明还提供了用于产生三胞胎细胞和四倍体细胞的方法，以及通过该方法产生的细胞。
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8.发明申请Applying Torque To Paramagnetic Structures In Bodies Using Dual Magnetic Fields 失效在使用双磁场的体中应用扭矩到顺磁结构公开(公告)号：US20120130149A1
公开(公告)日：2012-05-24
申请号：US13129453
申请日：2009-11-13
申请人： Paul Diament , Ilya Trakht
发明人： Paul Diament , Ilya Trakht
IPC分类号： A61N2/00
摘要： Techniques for applying torque to microscopic paramagnetic structures in a body includes exposing a body simultaneously to a first magnetic field oscillating at a first frequency in a first direction and a different second magnetic field oscillating at a second frequency in a second direction. The body includes a plurality of microscopic paramagnetic structures for which magnetic susceptibility to the first magnetic field is different from magnetic susceptibility to the second magnetic field. Such techniques are effective for ameliorating a symptom of a disease in an animal in which disease agents or diseased cells selectively include microscopic paramagnetic structures, including malaria.
摘要翻译： 将扭矩施加到体内的微观顺磁结构的技术包括将身体同时暴露于在第一方向上以第一频率振荡的第一磁场和在第二方向以第二频率振荡的不同的第二磁场。 主体包括多个微观顺磁结构，使第一磁场的磁化率与第二磁场的磁化率不同。 这样的技术对于改善动物中的疾病症状是有效的，其中疾病剂或病变细胞选择性地包括微观顺磁结构，包括疟疾。
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公开(公告)号：US20070154995A1
公开(公告)日：2007-07-05
申请号：US11704925
申请日：2007-02-09
申请人： Ilya Trakht
发明人： Ilya Trakht
CPC分类号： C07K16/3069 , C07K16/1235 , C07K16/2863 , C07K16/3015 , C07K2317/21 , C12N5/163 , C12N5/166 , C12N2510/02 , C12N2510/04 , G01N33/56983 , G01N33/57484 , G01N33/6893 , Y10S530/808 , Y10S530/809
摘要： The present invention provides a heteromyeloma cell other than B6B11, capable of producing a trioma cell when fused with a human lymphoid cell, wherein the trioma cell is capable of producing a tetroma cell capable of producing a monoclonal antibody having specific binding affinity for an antigen, when fused with a second human lymphoid cell, the second human lymphoid cell being capable of producing antibody having specific binding affinity for the antigen. The invention provides a trioma cell fusion partner which does not produce any antibody obtained by fusing a hetermomyeloma cell which does not produce any antibody with a human lymphoid cell. The invention provides a tetroma cell capable of producing a monoclonal antibody having specific binding affinity for an antigen obtained by fusing a trioma cell which does not produce any antibody with a human lymphoid cell capable of producing antibody having specific binding affinity for the antigen. The invention provides a method of producing a monoclonal antibody specific for an antigen associated with a condition. The invention provides a method of identifying an antigen associated with a condition using the trioma fusion partner. The invention provides a method of diagnosing a condition using the trioma fusion partner. The invention provides a method for preventing a condition. Compositions and therapeutic compositions are also provided, using monoclonal antibodies produced using the trioma fusion partner.
摘要翻译： 本发明提供了与人淋巴样细胞融合时能够产生三瘤细胞的B6B11以外的异源骨髓瘤细胞，其中三瘤细胞能够产生能产生对抗原具有特异性结合亲和力的单克隆抗体的四环细胞， 当与第二人淋巴细胞融合时，第二人淋巴细胞能够产生对抗原具有特异性结合亲和力的抗体。 本发明提供了不产生通过将不产生任何抗体的异源骨髓瘤细胞与人淋巴样细胞融合获得的任何抗体的三瘤细胞融合伴侣。 本发明提供能够产生对通过将不产生任何抗体的三瘤细胞与能够产生对抗原具有特异性结合亲和力的抗体的人淋巴细胞融合而获得的抗原具有特异性结合亲和力的单克隆抗体。 本发明提供了产生与病症相关的抗原特异性的单克隆抗体的方法。 本发明提供了鉴定与使用三聚体融合配偶体的病症相关的抗原的方法。 本发明提供了使用三聚体融合配偶体诊断病症的方法。 本发明提供一种防止病情的方法。 还使用使用三聚体融合配偶体产生的单克隆抗体来提供组合物和治疗组合物。
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公开(公告)号：US07220559B2
公开(公告)日：2007-05-22
申请号：US09767578
申请日：2001-01-23
申请人： Ilya Trakht
发明人： Ilya Trakht
CPC分类号： C07K16/3069 , C07K16/1235 , C07K16/2863 , C07K16/3015 , C07K2317/21 , C12N5/163 , C12N5/166 , C12N2510/02 , C12N2510/04 , G01N33/56983 , G01N33/57484 , G01N33/6893 , Y10S530/808 , Y10S530/809
摘要： This invention provides: a heteromyeloma, other than B6B11, capable of producing a trioma when fused with a human lymphoid cell, wherein the trioma is capable of producing a monoclonal antibody-secreting tetroma when fused with a second, antibody-secreting human lymphoid cell; a trioma fusion partner which does not produce antibody, obtained by fusing a heteromyeloma which does not produce antibody with a human lymphoid cell; a monoclonal antibody-secreting tetroma, obtained by fusing a trioma which does not produce antibody with an antibody-secreting human lymphoid cell; a method of producing a monoclonal antibody that specifically recognizes an antigen associated with a condition; a method of identifying an antigen associated with a condition using the trioma fusion partner; a method of diagnosing a condition using the trioma fusion partner; a method for preventing a condition; and compositions and therapeutic compositions comprising monoclonal antibodies produced using the trioma fusion partner.
摘要翻译： 本发明提供：当与人类淋巴样细胞融合时能够产生三胞胎的B6B11以外的异源骨髓瘤，其中当与第二种抗体分泌型人类淋巴样细胞融合时，三瘤能够产生单克隆抗体分泌性四唑; 通过将不产生抗体的异源骨髓瘤与人类淋巴样细胞融合得到的不产生抗体的三瘤融合伴侣; 通过将不产生抗体的三血症与抗体分泌型人淋巴样细胞融合获得的单克隆抗体分泌性四唑; 一种产生特异性识别与病症有关的抗原的单克隆抗体的方法; 使用三聚体融合配偶体鉴定与病症相关的抗原的方法; 使用三聚体融合伴侣诊断病症的方法; 一种预防病症的方法; 以及包含使用三聚体融合配偶体产生的单克隆抗体的组合物和治疗组合物。
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