公开(公告)号：US20240311638A1

公开(公告)日：2024-09-19

申请号：US18398161

申请日：2023-12-28

Applicant: Industrial Technology Research Institute

Inventor： Nien-Tzu Chou ,  Yu-Yu Lin ,  Ching-Fang Lu ,  Jian-Hao Li ,  Ting-Hsuan Chen ,  Cheng-Tai Chen

IPC: G06N3/0895 ,  G01N33/50 ,  G16B40/20

CPC classification number: G06N3/0895 ,  G01N33/5023 ,  G16B40/20 ,  G01N2333/70503 ,  G01N2333/70596 ,  G01N2333/7155

Abstract: A method of predicting the efficacy of natural killer cells, including: generating a plurality of training data corresponding to a plurality of donors based on a characteristic factor and a corresponding killing result against the target cancer cells of a plurality of cultured natural killer cells from the donors; obtaining a trained neural network model by inputting the plurality of training data into a neural network model; inputting a to-be-tested input vector corresponding to at least one characteristic factor of a to-be-tested natural killer cell into the trained neural network model to obtain an outputted result vector of the trained neural network model, wherein the result vector indicates a predicted killing result corresponding to the target cancer cell after applying the to-be-tested natural killer cell; and determining a quality of the to-be-tested natural killer cell based on the predicted killing result.

公开(公告)号：US09228231B2

公开(公告)日：2016-01-05

申请号：US13725273

申请日：2012-12-21

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Pei-Shin Jiang ,  Tzu-Hui Wu ,  Chia-Chun Chen ,  Su-Jan Lee ,  Chien-An Chen ,  Chien-Ming Hsu ,  Chung-Ya Liao ,  Yu-Yu Lin

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/6827 ,  C12Q2600/156

Abstract: One embodiment of the disclosure provides a kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence, including: at least one first primer consisting of a first segment and a second segment, wherein the first segment is a complementary strand of a first sequence and the second segment is a second sequence, and the 3′ end of the first segment connects to the 5′ end of the second segment; a second primer being a third sequence; at least one third primer consisting of a third segment and a fourth segment, wherein the third segment is a fourth sequence and the fourth segment is a complementary strand of a fifth sequence, and the 3′ end of the third segment connects to the 5′ end of the fourth segment; and a fourth primer being a complementary strand of a sixth sequence, wherein the specific region includes rs1799853, rs1057910, rs2108622, rs9923231 and rs9934438.

Abstract translation: 本公开的一个实施方案提供了用于检测靶核苷酸序列中特定区域的突变和/或多态性的试剂盒，其包括：至少一种由第一片段和第二片段组成的第一引物，其中第一片段是互补的 第二段是第二序列，第一段的3'端连接到第二段的5'端; 第二引物是第三序列; 至少一个第三引物由第三片段和第四片段组成，其中第三片段是第四序列，第四片段是第五序列的互补链，第三片段的3'末端连接到5' 第四段结束; 第四引物是第六序列的互补链，其中特异性区包括rs1799853，rs1057910，rs2108622，rs9923231和rs9934438。

公开(公告)号：US11920190B2

公开(公告)日：2024-03-05

申请号：US17134502

申请日：2020-12-28

Applicant: Industrial Technology Research Institute

Inventor： Pei-Shin Jiang ,  Jenn-Yeh Fann ,  Hung-Chi Chien ,  Yu-Yu Lin ,  Chih-Lung Lin

IPC: C12Q1/6855 ,  C12Q1/6869

CPC classification number: C12Q1/6855 ,  C12Q1/6869

Abstract: Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.

公开(公告)号：US20210214780A1

公开(公告)日：2021-07-15

申请号：US17134502

申请日：2020-12-28

Applicant: Industrial Technology Research Institute

Inventor： Pei-Shin Jiang ,  Jenn-Yeh Fann ,  Hung-Chi Chien ,  Yu-Yu Lin ,  Chih-Lung Lin

IPC: C12Q1/6855 ,  C12Q1/6869

Abstract: Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.