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公开(公告)号：US20230245808A1
公开(公告)日：2023-08-03
申请号：US18185346
申请日：2023-03-16
Applicant: Industrial Technology Research Institute
Inventor： Chien-An Chen , Cheng-Tai Chen , Pei-Shin Jiang
CPC classification number: H01F1/26 , H01F1/0054 , H01F1/37 , G01N2446/10 , G01N33/5434
Abstract: Described herein is a manufacturing method of a magnetic particle. First, deionized water, an organic solvent, a hydrophilic polymer, a lipid-soluble initiator, and at least two monomers are placed in a reactor and then stirred for polymerizing the at least two monomers into a copolymer to form a knobby copolymer core. Next, a polymer layer is formed to cover the knobby copolymer core, wherein the polymer layer has at least one functional group. Thereafter, a magnetic substance precursor is adsorbed by the knobby copolymer core covered with the polymer layer to form a magnetic substance layer. Further, a silicon-based layer may be additionally formed to cover the magnetic substance layer.
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2.发明申请公开(公告)号：US20210197162A1
公开(公告)日：2021-07-01
申请号：US17134496
申请日：2020-12-28
Applicant: Industrial Technology Research Institute
Inventor： Yu-Fang Yen , Chien-An Chen , Cheng-Tai Chen
Abstract: An extracellular vesicle separation method, a colloidal particle, and a preparation method thereof are provided. The colloidal particle is used for extracellular vesicle separation, and includes 2 wt % to 6 wt % of agarose. The colloidal particle has a particle size of 25 μm to 500 μm, and is surface-modified with biocompatible molecules. The biocompatible molecules include sodium carboxymethyl cellulose (CMC), methyl cellulose (MC), glycine, aspartic acid, glutamic acid, bovine serum albumin (BSA), fetal bovine serum (FBS), or a combination thereof.
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公开(公告)号：US20180164196A1
公开(公告)日：2018-06-14
申请号：US15381116
申请日：2016-12-16
Applicant: Industrial Technology Research Institute
Inventor： Chien-An Chen , Wen-Ching Lee , Tzu-Hui Wu , Pei-Shin Jiang , Ping-Jung Wu , Ruey-Shyan Hong , Hsiao-Jou Chang , Chun-Chieh Huang , Ting-Hsuan Chen , Chih-Lung Lin
CPC classification number: G01N1/36 , B01L3/508 , B01L7/52 , B01L2200/025 , B01L2200/0605 , B01L2200/0621 , B01L2200/0631 , B01L2300/0681 , B01L2300/0832 , B01L2300/0867 , B01L2400/0478 , B01L2400/0622 , B01L2400/0644 , C12M1/42 , C12N15/10 , G01N33/48
Abstract: A biological sample processing device includes a base, a purification unit, a metering unit and a first tube. The purification unit is disposed on the base and is configured to purify a sample. The metering unit is disposed on the base and has an inlet, at least one metering trough and an overflow trough. The inlet is connected to the purification unit via the first tube, and the metering trough is connected between the inlet and the overflow trough. The sample from the purification unit is configured to enter the metering unit through the inlet to be moved toward the metering trough, and to be moved toward the overflow trough after the metering trough is filled with the sample.
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公开(公告)号：US20220205993A1
公开(公告)日：2022-06-30
申请号：US17560276
申请日：2021-12-23
Applicant: Industrial Technology Research Institute
Inventor： Wen-Ting Chiang , Chien-An Chen , Cheng-Tai Chen
IPC: G01N33/543 , G01N33/58 , G01N33/53
Abstract: A detection method of multiple analytes includes the following. A microparticle is provided. The microparticle is coupled with at least one first ligand, and includes a body and a plurality of first protrusions formed on a surface of the body. Next, the microparticle is mixed with a variety of analytes to form a first complex. Thereafter, the first complex is mixed with a variety of second ligands carrying a variety of first labels, such that the variety of second ligands bind to the variety of analytes in the first complex and form a second complex. Lastly, the variety of first labels in the variety of second ligands in the second complex are detected.
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Patent Agency Ranking
公开(公告)号：US11859176B2
公开(公告)日：2024-01-02
申请号：US16228763
申请日：2018-12-21
Inventor： Cheng-Tai Chen , Chien-An Chen , Wen-Ting Chiang , Su-Feng Chiu , Pei-Shin Jiang , Jih-Luh Tang , Chien-Ting Lin , Xuan-Hui Lin
IPC: C12N5/00 , C12N13/00 , G01N33/543 , C12N5/0783 , C12N5/078 , A61K9/14
CPC classification number: C12N13/00 , C12N5/0634 , C12N5/0636 , C12N5/0646 , G01N33/54333 , C12N2501/505 , C12N2501/515 , C12N2501/998 , C12N2529/00 , C12N2533/10 , C12N2533/30 , C12N2535/00 , C12N2535/10
Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.
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公开(公告)号：US20190206598A1
公开(公告)日：2019-07-04
申请号：US16226651
申请日：2018-12-20
Applicant: Industrial Technology Research Institute
Inventor： Chien-An Chen , Cheng-Tai Chen , Pei-Shin Jiang
CPC classification number: H01F1/26 , G01N33/5434 , H01F1/0054 , H01F1/37
Abstract: Described herein is a magnetic particle, which includes a knobby copolymer core, a polymer layer, a magnetic substance layer and a silicon-based layer. The polymer layer covers the knobby copolymer core and has at least one functional group. In addition, the magnetic substance layer covers the polymer layer and the silicon-based layer covers the magnetic substance layer. Furthermore, a manufacturing method of the magnetic particle is also described.
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公开(公告)号：US20190203195A1
公开(公告)日：2019-07-04
申请号：US16228763
申请日：2018-12-21
Inventor： Cheng-Tai Chen , Chien-An Chen , Wen-Ting Chiang , Su-Feng Chiu , Pei-Shin Jiang , Jih-Luh Tang , Chien-Ting Lin , Xuan-Hui Lin
CPC classification number: C12N13/00 , C12N5/0634 , C12N5/0636 , C12N2501/505 , C12N2501/515 , C12N2501/998 , C12N2529/00 , C12N2533/10 , C12N2533/30 , C12N2535/00 , C12N2535/10 , G01N33/54333
Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.
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8.发明授权Kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence 有权用于检测靶核苷酸序列中特定区域的突变和/或多态性的试剂盒公开(公告)号：US09228231B2
公开(公告)日：2016-01-05
申请号：US13725273
申请日：2012-12-21
Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
Inventor： Pei-Shin Jiang , Tzu-Hui Wu , Chia-Chun Chen , Su-Jan Lee , Chien-An Chen , Chien-Ming Hsu , Chung-Ya Liao , Yu-Yu Lin
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , C12Q1/6827 , C12Q2600/156
Abstract: One embodiment of the disclosure provides a kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence, including: at least one first primer consisting of a first segment and a second segment, wherein the first segment is a complementary strand of a first sequence and the second segment is a second sequence, and the 3′ end of the first segment connects to the 5′ end of the second segment; a second primer being a third sequence; at least one third primer consisting of a third segment and a fourth segment, wherein the third segment is a fourth sequence and the fourth segment is a complementary strand of a fifth sequence, and the 3′ end of the third segment connects to the 5′ end of the fourth segment; and a fourth primer being a complementary strand of a sixth sequence, wherein the specific region includes rs1799853, rs1057910, rs2108622, rs9923231 and rs9934438.
Abstract translation: 本公开的一个实施方案提供了用于检测靶核苷酸序列中特定区域的突变和/或多态性的试剂盒，其包括：至少一种由第一片段和第二片段组成的第一引物，其中第一片段是互补的 第二段是第二序列，第一段的3'端连接到第二段的5'端; 第二引物是第三序列; 至少一个第三引物由第三片段和第四片段组成，其中第三片段是第四序列，第四片段是第五序列的互补链，第三片段的3'末端连接到5' 第四段结束; 第四引物是第六序列的互补链，其中特异性区包括rs1799853，rs1057910，rs2108622，rs9923231和rs9934438。
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公开(公告)号：US11884912B2
公开(公告)日：2024-01-30
申请号：US16727057
申请日：2019-12-26
Applicant: Industrial Technology Research Institute
Inventor： Kuo-Hsing Wen , Ting-Hsuan Chen , Cheng-Tai Chen , Chien-An Chen , Su-Fung Chiu , Yung-Chi Chang , Nien-Jen Chou , Ping-Jung Wu , Shaw-Hwa Parng , Pei-Shin Jiang
Abstract: A cell culture device includes a culture unit, a gas supply unit, a first pressure unit, at least one inspecting unit and a control unit. The culture unit contains a cell culture liquid. The gas supply unit, connected with the culture unit, is used for transmitting a culture gas into the culture unit. The first pressure unit, connected with the culture unit, is used for applying a pressure to the cell culture liquid in the culture unit. The at least one inspecting unit, connected with the culture unit, is used for receiving the cell culture liquid for inspection. The control unit, electrically coupled with the culture unit, the first pressure unit, the gas supply unit and the at least one inspecting unit, is used for monitoring corresponding condition parameters to determine respective operations. In addition, a cell culture method for the cell culture device is also provided.
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