Transcriptional factor enhancing the resistance of plants to osmotic stress
    1.
    发明授权
    Transcriptional factor enhancing the resistance of plants to osmotic stress 有权
    转录因子增强植物对渗透胁迫的抵抗力

    公开(公告)号:US07141720B2

    公开(公告)日:2006-11-28

    申请号:US10433005

    申请日:2001-03-09

    摘要: The present invention relates to a transcription factor of a plant, AtSIZ, induced by osmotic stress; a gene encoding the transcription factor; and a method for enhancing resistance to stress of a plant by using the gene. The AtSIZ protein isolated from Arabidopsis thaliana is a polypeptide, which contains a C3H-type zinc finger motif and acts as a transcription regulation factor activating transcription of genes related to stress response of a plant. Therefore, a plant with the enhanced resistance to osmotic stress can be produced by over expressing the AtSIZ gene in the plant through transforming recombinant plasmid containing AtSIZ gene into a plant and consequently, the productivity of the plant can be increased.

    摘要翻译: 本发明涉及由渗透胁迫诱导的植物AtSIZ的转录因子; 编码转录因子的基因; 以及通过使用该基因来提高植物的胁迫抗性的方法。 从拟南芥分离的AtSIZ蛋白是一种多肽,其含有C 3 H型锌指基序,并且作为激活与植物的应激反应有关的基因的转录的转录调节因子。 因此,通过将含有AtSIZ基因的重组质粒转化到植物中,可以通过过度表达植物中的AtSIZ基因来产生具有增强的渗透胁迫抗性的植物,从而可以提高植物的生产力。

    System for detecting protease
    2.
    发明申请
    System for detecting protease 有权
    蛋白酶检测系统

    公开(公告)号:US20070269812A1

    公开(公告)日:2007-11-22

    申请号:US11506143

    申请日:2006-08-16

    IPC分类号: C12Q1/68 C07H21/04 C12N15/63

    摘要: Disclosed is a system for detecting a protease inside a cell. In one embodiment, the system includes a chimeric protein that comprises as covalently linked components: 1) at least one optionally masked signal protein; 2) at least one protease-specific cleavage site; and 3) at least one detectable amino acid sequence. The invention has a wide spectrum of applications including use in the detection of novel protease inhibitors inside cells and tissue.

    摘要翻译: 公开了一种用于检测细胞内的蛋白酶的系统。 在一个实施方案中,该系统包括嵌合蛋白,其包含作为共价连接的组分:1)至少一种任选地掩蔽的信号蛋白; 2)至少一个蛋白酶特异性切割位点; 和3)至少一个可检测的氨基酸序列。 本发明具有广泛的应用,包括用于检测细胞和组织内的新型蛋白酶抑制剂。

    USE OF AtBG1 GENE FOR THE GENERATION OF TRANSGENIC PLANTS WITH INCREASED RESISTANCE TO VARIOUS ABIOTIC STRESS
    3.
    发明申请
    USE OF AtBG1 GENE FOR THE GENERATION OF TRANSGENIC PLANTS WITH INCREASED RESISTANCE TO VARIOUS ABIOTIC STRESS 有权
    AtBG1基因用于产生具有抗各向异性胁迫抗性的转基因植物

    公开(公告)号:US20090138983A1

    公开(公告)日:2009-05-28

    申请号:US11722578

    申请日:2005-12-22

    摘要: The present invention relates to a use of AtBG1 {Arabidopsis thaliana β-glucosidase 1) gene and a transgenic plan with the AtBG1 gene insertion, more precisely, a use of AtBG1 gene for the generation of a transgenic plant having resistance to various environmental stresses by increasing the level of abscisic acid (ABA), a kind of plant hormone, and a transgenic plant having stress-resistance with the AtBG1 gene insertion. Plants have enhanced resistance against various environmental stresses including low temperature, salt damage and dehydration owing to the increased level of ABA. Thus, the method to increase the level of ABA can greatly contribute to the enhancement of agricultural production.

    摘要翻译: 本发明涉及AtBG1(拟南芥β-糖苷酶1)基因的用途和具有AtBG1基因插入的转基因方案,更确切地说,涉及使用AtBG1基因产生具有各种环境胁迫抗性的转基因植物 增加脱落酸(ABA)的水平,一种植物激素和具有AtBG1基因插入的抗应激性的转基因植物。 由于ABA水平的提高,植物对各种环境胁迫具有增强的抗性,包括低温,盐损害和脱水。 因此,提高ABA水平的方法可以大大有助于提高农业生产。

    System for detecting protease
    5.
    发明授权
    System for detecting protease 有权
    蛋白酶检测系统

    公开(公告)号:US07855072B2

    公开(公告)日:2010-12-21

    申请号:US11506143

    申请日:2006-08-16

    IPC分类号: C07H17/00 C07K14/00

    摘要: Disclosed is a system for detecting a protease inside a cell. In one embodiment, the system includes a chimeric protein that comprises as covalently linked components: 1) at least one optionally masked signal protein; 2) at least one protease-specific cleavage site; and 3) at least one detectable amino acid sequence. The invention has a wide spectrum of applications including use in the detection of novel protease inhibitors inside cells and tissue.

    摘要翻译: 公开了一种用于检测细胞内的蛋白酶的系统。 在一个实施方案中,该系统包括嵌合蛋白,其包含作为共价连接的组分:1)至少一种任选地掩蔽的信号蛋白; 2)至少一个蛋白酶特异性切割位点; 和3)至少一个可检测的氨基酸序列。 本发明具有广泛的应用,包括用于检测细胞和组织内的新型蛋白酶抑制剂。

    Genetic modification of plants for enhanced resistance and decreased uptake of heavy metals
    6.
    发明申请
    Genetic modification of plants for enhanced resistance and decreased uptake of heavy metals 审中-公开
    植物的遗传修饰增强了抗性,降低了重金属的吸收

    公开(公告)号:US20060130173A1

    公开(公告)日:2006-06-15

    申请号:US10756047

    申请日:2004-01-12

    CPC分类号: C12N9/14 C12N15/8271

    摘要: The present invention relates to a method of producing transformants with enhanced resistance and decreased uptake of heavy metals, and a plant transformed with a P type ATPase ZntA gene that pumps out heavy metals from the cells. The transformants show better growth than wild type in environment contaminated with heavy metals and have lower heavy metal contents than wild type plants. Therefore, this method of transforming plants with ZntA or biologically active ZntA-like heavy metal pumping ATPases can be useful for developing plants for phytoremediation and also for a safe crop that has resistance to heavy metals and low heavy metal contents.

    摘要翻译: 本发明涉及一种生产具有增强的抗性和降低重金属摄取的转化体的方法,以及用P型ATP酶ZntA基因转化的植物,其从细胞中抽出重金属。 转基因在重金属污染的环境中显示出比野生型更好的生长,并且比野生型植物具有更低的重金属含量。 因此,这种用ZntA或生物活性的ZntA样重金属泵送ATP酶转化植物的方法可用于开发用于植物修复的植物以及对重金属和低重金属含量具有抗性的安全作物。

    Use of AtBG1 gene for the generation of transgenic plants with increased resistance to various abiotic stress
    7.
    发明授权
    Use of AtBG1 gene for the generation of transgenic plants with increased resistance to various abiotic stress 有权
    使用AtBG1基因产生转基因植物,对各种非生物胁迫具有增加的抗性

    公开(公告)号:US07795498B2

    公开(公告)日:2010-09-14

    申请号:US11722578

    申请日:2005-12-22

    IPC分类号: C12N15/82 C12N5/14 C07H21/04

    摘要: The present invention relates to a use of AtBG1 {Arabidopsis thaliana β-glucosidase 1) gene and a transgenic plan with the AtBG1 gene insertion, more precisely, a use of AtBG1 gene for the generation of a transgenic plant having resistance to various environmental stresses by increasing the level of abscisic acid (ABA), a kind of plant hormone, and a transgenic plant having stress-resistance with the AtBG1 gene insertion. Plants have enhanced resistance against various environmental stresses including low temperature, salt damage and dehydration owing to the increased level of ABA. Thus, the method to increase the level of ABA can greatly contribute to the enhancement of agricultural production.

    摘要翻译: 本发明涉及AtBG1(拟南芥和β-葡糖苷酶1)基因的用途和具有AtBG1基因插入的转基因计划,更确切地说,涉及使用AtBG1基因产生具有各种环境胁迫抗性的转基因植物 通过增加脱落酸(ABA)的水平,一种植物激素和具有AtBG1基因插入的抗应激性的转基因植物。 由于ABA水平的提高,植物对各种环境胁迫具有增强的抗性,包括低温,盐损害和脱水。 因此,提高ABA水平的方法可以大大有助于提高农业生产。

    System for detecting protease
    8.
    发明授权

    公开(公告)号:US07109293B2

    公开(公告)日:2006-09-19

    申请号:US10214932

    申请日:2002-08-08

    IPC分类号: C07K14/00

    摘要: Disclosed is a system for detecting a protease inside a cell. In one embodiment, the system includes a chimeric protein that comprises as covalently linked components: 1) at least one optionally masked signal protein; 2) at least one protease-specific cleavage site; and 3) at least one detectable amino acid sequence. The invention has a wide spectrum of applications including use in the detection of novel protease inhibitors inside cells and tissue.