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    Mutant protein having the peptide-synthesizing activity
    3.
    发明申请
    Mutant protein having the peptide-synthesizing activity 审中-公开
    具有肽合成活性的突变蛋白

    公开(公告)号：US20060188976A1

    公开(公告)日：2006-08-24

    申请号：US11311467

    申请日：2005-12-20

    申请人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

    发明人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

    IPC分类号： C12N9/48 ,  C07H21/04 ,  C12P21/06 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C07K14/00 ,  C07K14/195 ,  C12N9/93

    摘要： The present invention is to provide an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. A peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II): (I) The mutant protein having the amino acid sequence containing one or more mutations of the above mutations 1 to 38 in the amino acid sequence described in SEQ ID NO:2; and (II) The mutant protein having the amino acid sequence containing one or more mutations selected from the group consisting of substitution, deletion, insertion, addition and inversion at positions other than one or more mutation positions of the above mutation 1 to 38 in the mutant protein described in the above (I), and having a peptide-synthesizing activity.

    摘要翻译： 本发明提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质的存在下，使胺组分和羧基组分反应合成肽：（I）具有含有一个或多个突变的氨基酸序列的突变蛋白 在SEQ ID NO：2中描述的氨基酸序列中的上述突变1至38; 和（II）具有在上述变异株1〜38中的一个以上突变位置以外的位置上具有选自取代，缺失，插入，添加和倒位的一个或多个突变的氨基酸序列的突变蛋白质 上述（I）所述的突变型蛋白质，具有肽合成活性。

    METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME
    4.
    发明申请
    METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME 有权
    生产用于其的丝氨酸衍生物和蛋白质的方法

    公开(公告)号：US20100317068A1

    公开(公告)日：2010-12-16

    申请号：US12836984

    申请日：2010-07-15

    申请人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    发明人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    IPC分类号： C12P13/06 ,  C07H21/00 ,  C12N9/10 ,  C12N1/21

    CPC分类号： C12N9/1029 ,  C12P13/04

    摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.

    摘要翻译： 本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。 在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质 （B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物 。

    Method for producing serine derivative and protein used for the same
    5.
    发明授权
    Method for producing serine derivative and protein used for the same 有权
    用于制备丝氨酸衍生物和蛋白质的方法

    公开(公告)号：US08372607B2

    公开(公告)日：2013-02-12

    申请号：US12836984

    申请日：2010-07-15

    申请人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    发明人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    IPC分类号： C12P13/06 ,  C07H21/00 ,  C12N1/21

    CPC分类号： C12N9/1029 ,  C12P13/04

    摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.

    摘要翻译： 本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。 在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质 （B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物 。

    METHOD FOR PRODUCING BETA-ALANYL-AMINO ACID OR DERIVATIVE THEREOF
    9.
    发明申请
    METHOD FOR PRODUCING BETA-ALANYL-AMINO ACID OR DERIVATIVE THEREOF 审中-公开
    生产β-氨基酸或其衍生物的方法

    公开(公告)号：US20110081678A1

    公开(公告)日：2011-04-07

    申请号：US12943509

    申请日：2010-11-10

    申请人： Rie Takeshita ,  Yasuaki Takakura ,  Shunichi Suzuki ,  Kenzo Yokozeki

    发明人： Rie Takeshita ,  Yasuaki Takakura ,  Shunichi Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P21/02 ,  C07H21/04 ,  C12N9/00 ,  C12N5/10

    CPC分类号： C12P21/02 ,  C07K5/0202 ,  C12N9/48

    摘要： The present invention provides a method for efficiently producing a β-alanyl-amino acid (e.g., carnosine) or derivative thereof. Specifically, it provides a method for producing a β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof, by reacting a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof in the presence of an enzyme or an enzyme-containing product that has an ability to form the β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof from the β-alanyl ester or the β-alanyl amide and the amino acid (e.g., histidine) or derivative thereof. The present invention also provides a protein which is able to catalyze production of a β-alanyl-amino acid or derivative thereof from a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof, and a polynucleotide encoding said protein.

    摘要翻译： 本发明提供了一种有效生产β-丙氨酰 - 氨基酸（如肌肽）或其衍生物的方法。 具体地说，本发明提供了一种通过使β-丙氨酰基酯或一种 - 丙氨酰基酰胺与氨基酸或氨基酸反应生产β-丙氨酰 - 氨基酸（例如， - 丙氨酰 - 组氨酸）或其衍生物的方法 其衍生物在酶或含酶产物的存在下具有从丙氨酰酯形成β-淀粉氨基酸（例如，β-丙氨酰 - 组氨酸）或其衍生物的能力，或 丙氨酰酰胺和氨基酸（如组氨酸）或其衍生物。 本发明还提供一种蛋白质，其能够催化β-氨基丙氨酰 - 氨基酸或其衍生物从β-丙氨酰酯或其 - 氨基酸或其衍生物及其衍生物的产生，以及多核苷酸 编码所述蛋白质。

    Process for preparing xylitol
    10.
    发明授权
    Process for preparing xylitol 失效
    制备木糖醇的方法

    公开(公告)号：US06303353B1

    公开(公告)日：2001-10-16

    申请号：US09509547

    申请日：2000-04-12

    申请人： Masakazu Sugiyama ,  Shunichi Suzuki ,  Yasuhiro Mihara ,  Kenichi Hashiguchi ,  Kenzo Yokozeki

    发明人： Masakazu Sugiyama ,  Shunichi Suzuki ,  Yasuhiro Mihara ,  Kenichi Hashiguchi ,  Kenzo Yokozeki

    IPC分类号： C12P718

    CPC分类号： C12P7/18

    摘要： Xylitol is produced by contacting D-arabitol with a microorganism that belongs to the genus Gluconobacter, Acetobacter, Achromobacter, Agrobacterium, Arthrobacter, Azotobacter, Brevibacterium, Corynebacterium, Erwinia, Flavobacterium, Micrococcus, Nocardia, Planococcus, Pseudomonas, Rhodococcus, Morganella, Actinomadura, Actinomyces, or Streptomyces and is capable of converting D-arabitol to xylitol, and recovering xylitol thus produced.

    摘要翻译： 通过使D-阿拉伯糖醇与属于葡萄糖酸杆菌属，醋杆菌属，无色杆菌属，农杆菌属，节杆菌属，固氮菌属，短杆菌属，棒状杆菌属，欧文氏菌属，黄杆菌属，微球菌属，诺卡氏菌属，普兰克菌属，假单胞菌属，红球菌属，摩根氏菌属，放线菌属， 放线菌或链霉菌，能够将D-阿拉伯糖醇转化为木糖醇，并回收如此生产的木糖醇。