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公开(公告)号：US20070190602A1
公开(公告)日：2007-08-16
申请号：US11316926
申请日：2005-12-27
申请人： Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人： Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号： C12N9/93
摘要： The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II).(I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2.(II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译： 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质存在下，使胺组分和羧基组分反应合成肽。 （I）具有包含SEQ ID NO：2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 （II）具有包含SEQ ID NO：208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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公开(公告)号：US20070292916A1
公开(公告)日：2007-12-20
申请号：US11765926
申请日：2007-06-20
申请人： Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人： Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号： C07K14/00 , C07K14/195 , C12N9/93
摘要： The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2. (II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译： 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质存在下，使胺组分和羧基组分反应合成肽。 （I）具有包含SEQ ID NO：2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 （II）具有包含SEQ ID NO：208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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公开(公告)号：US20090264338A1
公开(公告)日：2009-10-22
申请号：US11849397
申请日：2007-09-04
CPC分类号： C07K14/001 , A61K38/28 , A61K47/183 , A61K47/42 , C07K14/62
摘要： An insulin preparation having an ultra-rapid onset of action is provided by adding a substance that interacts with the insulin dimer formation surface or the hexamer formation surface to an insulin solution. The substance exerts its effect by inhibiting insulin dimer formation and/or hexamer formation.
摘要翻译： 通过将与胰岛素二聚体形成表面或六聚体形成表面相互作用的物质与胰岛素溶液相加来提供具有超快速起效作用的胰岛素制剂。 该物质通过抑制胰岛素二聚体形成和/或六聚体形成而发挥其作用。
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4.发明授权Variant aldolase and processes for producing an optically active IHOG and an optically active monatin using the same 失效变体醛缩酶和使用其制备光学活性的IHOG和光学活性莫纳甜的方法公开(公告)号：US07432100B2
公开(公告)日：2008-10-07
申请号：US11080628
申请日：2005-03-16
CPC分类号： C12P17/10 , C07K2299/00 , C12N9/88
摘要： The present invention relates to variant aldolase enzymes that are modified so as to produce IHOG (4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid: IHOG), a process for producing an optically active IHOG using the same, and a process for producing an optically active monatin.
摘要翻译： 本发明涉及修饰以产生IHOG（4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸：IHOG）的变体醛缩酶，使用其制备光学活性的IHOG的方法 ，以及制备光学活性莫纳甜的方法。
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5.发明申请Variant aldolase and processes for producing an optically active IHOG and an optically active monatin using the same 失效变体醛缩酶和使用其制备光学活性的IHOG和光学活性莫纳甜的方法公开(公告)号：US20050244939A1
公开(公告)日：2005-11-03
申请号：US11080628
申请日：2005-03-16
IPC分类号： C12N15/09 , C07H21/04 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N9/88 , C12N15/74 , C12P17/10
CPC分类号： C12P17/10 , C07K2299/00 , C12N9/88
摘要： The present invention relates to variant aldolase enzymes that are modified so as to produce IHOG, a process for producing an optically active IHOG using the same, and a process for producing an optically active monatin.
摘要翻译： 本发明涉及修饰以产生IHOG的变体醛缩酶，使用其制备具有光学活性的IHOG的方法和用于制备光学活性莫纳甜的方法。
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6.发明授权公开(公告)号：US06710031B2
公开(公告)日：2004-03-23
申请号：US09969763
申请日：2001-10-04
申请人： Naoyuki Fukuchi , Morikazu Kito , Takashi Kayahara , Fumie Futaki , Kohki Ishikawa , Eiichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Naoyuki Yamada
发明人： Naoyuki Fukuchi , Morikazu Kito , Takashi Kayahara , Fumie Futaki , Kohki Ishikawa , Eiichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Naoyuki Yamada
IPC分类号： A61K3846
CPC分类号： C07K14/46 , C07K14/745
摘要： A method for producing a protein having an antithrombotic activity, which comprises replacing, in a protein that has an amino acid sequence having a homology of not less than 30% to the amino acid sequence of SEQ ID NO: 1 and forms a higher order structure composed of a first &bgr; strand (&bgr;1), a first &agr; helix (&agr;1), a second &agr; helix (&agr;2), a second &bgr; strand (&bgr;2), a loop, a third &bgr; strand (&bgr;3), a fourth &bgr; strand (&bgr;4) and a fifth &bgr; strand (&bgr;5) in this order from the amino terminus, at least one amino acid residue in a region from &agr;2 to &bgr;2 and/or a region from &bgr;3 to &bgr;4 so that electric charge of the amino acid residue is changed towards positive direction.
摘要翻译： 一种具有抗血栓形成活性的蛋白质的制造方法，其特征在于，具有与SEQ ID NO：1的氨基酸序列具有不小于30％的同源性的氨基酸序列的蛋白质，并形成高级结构 由第一β链（β1），第一α螺旋（alpha1），第二α螺旋（α2），第二β链（β2），环，第三β链（β3），第四条β链 β4）和第五个β链（β5），从氨基末端起始，α2至β2区域和/或β3至β4区域中的至少一个氨基酸残基，使得氨基酸残基的电荷为 转向正向。
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公开(公告)号：US06762041B2
公开(公告)日：2004-07-13
申请号：US09850031
申请日：2001-05-08
IPC分类号： C12P1314
CPC分类号： C07K5/06104 , G01N33/534 , G01N2333/9108
摘要： The present invention provides a method for isotopically labeling a functional group possessed by an amino acid residue of a protein. The present invention also provides a protein whose functional group in an amino acid residue is isotopically labeled. A functional group in an amino acid residue of a protein is substituted with an isotope-labeling group derived from an isotope-labeling compound by making use of the action of an enzyme. In particular, the carboxyamide nitrogen atom in a glutamine residue of a protein is replaced with an isotopically labeled atom by acting a transglutaminase on the glutamine residue.
摘要翻译： 本发明提供一种同位素标记由蛋白质的氨基酸残基所具有的官能团的方法。 本发明还提供一种蛋白质，其氨基酸残基中的官能团被同位素标记。蛋白质的氨基酸残基中的官能团被来自同位素标记化合物的同位素标记基团取代， 酶的作用 特别地，蛋白质的谷氨酰胺残基中的羧酰胺氮原子被同位素标记的原子取代，通过在谷氨酰胺残基上起转谷氨酰胺酶的作用。
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8.发明授权公开(公告)号：US07084113B2
公开(公告)日：2006-08-01
申请号：US10749387
申请日：2004-01-02
申请人： Naoyuki Fukuchi , Morikazu Kito , Takashi Kayahara , Fumie Futaki , Kohki Ishikawa , Eiichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Naoyuki Yamada
发明人： Naoyuki Fukuchi , Morikazu Kito , Takashi Kayahara , Fumie Futaki , Kohki Ishikawa , Eiichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Naoyuki Yamada
CPC分类号： C07K14/46 , C07K14/745
摘要： A method for producing a protein having an antithrombotic activity, which comprises replacing, in a protein that has an amino acid sequence having a homology of not less than 30% to the amino acid sequence of SEQ ID NO: 1 and forms a higher order structure composed of a first β strand (β1), a first a helix (α1), a second α helix (α2), a second β strand (β2), a loop, a third β strand (β3), a fourth β strand (β4) and a fifth β strand (β5) in this order from the amino terminus, at least one amino acid residue in a region from α2 to β2 and/or a region from β3 to β4 so that electric charge of the amino acid residue is changed towards positive direction.
摘要翻译： 一种具有抗血栓形成活性的蛋白质的制造方法，其特征在于，具有与SEQ ID NO：1的氨基酸序列具有不小于30％的同源性的氨基酸序列的蛋白质，并形成高级结构 由第一个β链（beta1），第一个螺旋（alpha1），第二个α螺旋（alpha2），第二个β链（β2），一个环，第三个β链（β3），第四个β链 β4）和第五个β链（β5），从氨基末端起始，α2至β2区域和/或β3至β4区域中的至少一个氨基酸残基，使得氨基酸残基的电荷为 转向正向。
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公开(公告)号：US20110064847A1
公开(公告)日：2011-03-17
申请号：US12881466
申请日：2010-09-14
申请人： Noriko Miwa , Nobuhisa Shimba , Mina Nakamura , Eiichiro Suzuki , Keiichi Yokoyama , Hiroyuki Nakagoshi , Fumiyuki Hirose , Hiroaki Sato
发明人： Noriko Miwa , Nobuhisa Shimba , Mina Nakamura , Eiichiro Suzuki , Keiichi Yokoyama , Hiroyuki Nakagoshi , Fumiyuki Hirose , Hiroaki Sato
CPC分类号： A23J3/34 , A23J3/04 , A23J3/06 , A23J3/08 , A23J3/16 , A23J3/18 , A23V2002/00 , C12Y203/02013 , C12Y305/01002 , A23V2250/5422 , A23V2300/08 , A23V2250/5424 , A23V2250/54252 , A23V2250/5432 , A23V2250/5486 , A23V2250/5488
摘要： A method of denaturing a protein by treating the protein with a protein glutaminase and a transglutaminase, a food containing a protein having been denatured with these enzymes, and an enzyme preparation for denaturing a protein which contains these enzymes. A protein is denatured by adding protein glutaminase and transglutaminase to the protein substantially at the same timing, or adding protein glutaminase to the protein before the transglutaminase acts on the protein, or controlling the quantitative ratio of protein glutaminase to transglutaminase, by which a protein is treated, to a definite level.
摘要翻译： 通过用蛋白质谷氨酰胺酶和转谷氨酰胺酶处理蛋白质来改变蛋白质的方法，含有已经用这些酶变性的蛋白质的食物，以及用于使包含这些酶的蛋白质变性的酶制剂。 通过将蛋白质谷氨酰胺酶和转谷氨酰胺酶基本上同时加入到蛋白质中，或者在转谷氨酰胺酶作用于蛋白质之前向蛋白质中加入蛋白质谷氨酰胺酶，或控制蛋白质谷氨酰胺酶与转谷氨酰胺酶的定量比例，蛋白质变性 处理到一定程度。
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公开(公告)号：US20100143970A1
公开(公告)日：2010-06-10
申请号：US12541220
申请日：2009-08-14
申请人： Keiichi Yokoyama , Mototaka Suzuki , Tatsuki Kashiwagi , Eiichiro Suzuki , Masayo Date , Seiichi Taguchi
发明人： Keiichi Yokoyama , Mototaka Suzuki , Tatsuki Kashiwagi , Eiichiro Suzuki , Masayo Date , Seiichi Taguchi
CPC分类号： C12N9/1044 , C12Y203/02013
摘要： A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).
摘要翻译： 突变以具有改善的耐热性和/或pH稳定性的转谷氨酰胺酶蛋白。 在能够形成二硫键的半胱氨酸残基（SS键）上引入WT转谷氨酰胺酶的突变。
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