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    MODIFICATION OF AMYLOID-BETA LOAD IN NON-BRAIN TISSUE
    6.
    发明申请
    MODIFICATION OF AMYLOID-BETA LOAD IN NON-BRAIN TISSUE 审中-公开
    非脑组织中AMYLOID-BETA载体的修饰

    公开(公告)号：US20100120787A1

    公开(公告)日：2010-05-13

    申请号：US12618656

    申请日：2009-11-13

    申请人： J. Gregor Sutcliffe ,  Floyd E. Bloom ,  Brian S. Hilbush

    发明人： J. Gregor Sutcliffe ,  Floyd E. Bloom ,  Brian S. Hilbush

    IPC分类号： A61K31/496 ,  C12Q1/68 ,  A61P25/28 ,  A61K31/506 ,  A61K31/519

    CPC分类号： A61K31/506 ,  A61K9/0053 ,  A61K31/00 ,  A61K31/496 ,  A61K45/06 ,  C12N15/113 ,  C12N2310/14 ,  G01N33/6896 ,  G01N2333/4709 ,  G01N2800/2821

    摘要： The present invention relates to methods and compositions for modulating levels of amyloid-β peptide (Aβ) exhibited by non-neuronal (i.e., peripheral) cells, fluids, or tissues. The invention also relates to modulation of Aβ levels via selective modulation (e.g., inhibition) of γ-secretase activity. The invention also relates to methods of preventing, treating or ameliorating the symptoms of a disorder, including but not limited to an Aβ-related disorder, by administering a compound that result in the modulation of γ-secretase in a non-neuronal tissue, either directly or indirectly to prevent, treat or ameliorate the symptoms of a brain Aβ disorder, such as Alzheimer's disease.

    摘要翻译： 本发明涉及调节淀粉样蛋白水平的方法和组合物， 由非神经元（即外周）细胞，流体或组织表现的肽（A＆bgr）。 本发明还涉及A＆Bgr的调制。 通过γ-分泌酶活性的选择性调节（例如抑制）。 本发明还涉及通过在非神经元组织中施用导致γ-分泌酶调节的化合物来预防，治疗或改善病症症状，包括但不限于有关疾病的方法， 直接或间接地预防，治疗或改善大脑A＆BGR的症状; 障碍，如阿尔茨海默病。

    Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
    7.
    发明授权
    Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations 失效
    同时鉴别差异表达mRNA的方法和相对浓度的测定

    公开(公告)号：US06309834B1

    公开(公告)日：2001-10-30

    申请号：US09316349

    申请日：1999-05-21

    申请人： J. Gregor Sutcliffe ,  Mark G. Erlander

    发明人： J. Gregor Sutcliffe ,  Mark G. Erlander

    IPC分类号： C12Q168

    CPC分类号： C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6853 ,  C12Q2600/158 ,  C12Q2545/114 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2525/185 ,  C12Q2525/173 ,  C12Q2525/131

    摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3′-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3′-primer whose sequence is derived from the vector and a set of 5′-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.

    摘要翻译： 用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。 通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组引物从cRNA转录cDNA，并使用序列衍生自载体的3'-引物进行PCR，以及从用于从cRNA转录cDNA的引物衍生的一组5'-引物 。 该方法可以鉴定与给药药物或与生理或病理状况相关的mRNA的表达变化。

    Method for simulataneous identification of differentially expresed mRNAS and measurement of relative concentrations
    8.
    发明授权
    Method for simulataneous identification of differentially expresed mRNAS and measurement of relative concentrations 失效
    用于模拟鉴别差异表达mRNAs的方法和相对浓度的测量

    公开(公告)号：US5807680A

    公开(公告)日：1998-09-15

    申请号：US544577

    申请日：1995-10-17

    申请人： J. Gregor Sutcliffe ,  Mark G. Erlander

    发明人： J. Gregor Sutcliffe ,  Mark G. Erlander

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N15/10 ,  C12P19/34 ,  C12P21/02 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/19 ,  G01N33/48

    CPC分类号： C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6853 ,  C12Q2600/158

    摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.

    摘要翻译： 用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。 通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组引物从cRNA转录cDNA，并使用序列衍生自载体的3'-引物进行PCR，以及从用于从cRNA转录cDNA的引物衍生的一组5'-引物 。 该方法可以鉴定与给药药物或生理或病理状况相关的mRNA的表达变化。

    Method for simultaneous identification of differentially expressed mRNAS and measurement of relative concentrations
    9.
    发明授权
    Method for simultaneous identification of differentially expressed mRNAS and measurement of relative concentrations 失效
    同时鉴别差异表达mRNAs的方法和相对浓度的测定

    公开(公告)号：US6030784A

    公开(公告)日：2000-02-29

    申请号：US35190

    申请日：1998-03-05

    申请人： J. Gregor Sutcliffe ,  Mark G. Erlander

    发明人： J. Gregor Sutcliffe ,  Mark G. Erlander

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N15/10 ,  C12P19/34 ,  C12P21/02 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/19 ,  G01N33/48

    CPC分类号： C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6853 ,  C12Q2600/158

    摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.

    摘要翻译： 用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。 通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组引物从cRNA转录cDNA，并使用序列衍生自载体的3'-引物进行PCR，以及从用于从cRNA转录cDNA的引物衍生的一组5'-引物 。 该方法可以鉴定与给药药物或生理或病理状况相关的mRNA的表达变化。