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1.发明申请MEDIUM, FOR CULTURING CORNEAL ENDOTHELIAL CELLS, CONTAINING CONDITIONED MEDIUM FROM MESENCHYMAL STEM CELLS 审中-公开用于培养角膜内皮细胞的培养基，含有来自中胚层干细胞的调节培养基公开(公告)号：US20150210981A1
公开(公告)日：2015-07-30
申请号：US14425354
申请日：2013-09-05
发明人： Michio Hagiya , Kiwamu Imagawa , Yuuki Hosoda , Noriko Koizumi , Naoki Okumura , Makiko Nakahara , Shigeru kinoshita
CPC分类号： C12N5/0621 , A61K35/30 , A61K35/44 , C12N5/0018 , C12N2500/84 , C12N2502/1358
摘要： [Problem] To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.
摘要翻译： 为了提供将细胞移植到患有角膜内皮细胞衰竭的患者中的介质，用于培养从角膜组织获得的角膜内皮细胞培养的培养基，并且在将其形态保持为角膜内皮细胞的同时生长所述细胞。 [溶液]含有来自间充质干细胞的条件培养基的培养基; 以及使用所述培养基培养角膜内皮细胞的方法。
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公开(公告)号：US10174284B2
公开(公告)日：2019-01-08
申请号：US14425354
申请日：2013-09-05
发明人： Michio Hagiya , Kiwamu Imagawa , Yuuki Hosoda , Noriko Koizumi , Naoki Okumura , Makiko Nakahara , Shigeru Kinoshita
摘要： [Problem] To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.
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公开(公告)号：US10335439B2
公开(公告)日：2019-07-02
申请号：US14772854
申请日：2014-03-09
发明人： Kiwamu Imagawa , Kenichi Maeda , Yuki Hosoda , Shuichi Yokoyama , Naoki Okumura , Noriko Koizumi , Shigeru Kinoshita
摘要： [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate.[Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-β receptor inhibitor in various combinations.
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公开(公告)号：US20150202256A1
公开(公告)日：2015-07-23
申请号：US14413066
申请日：2013-07-03
申请人： Kyoto Prefectural Public University Corporation , The Doshisha , Senju Pharmaceutical Co., Ltd.
发明人： Takahiro Nakamura , Noriko Koizumi , Naoki Okumura , Shigeru Kinoshita , Kana Tominaga , Satoshi Kawasaki
CPC分类号： A61K38/1709 , A61K35/30 , A61K38/00 , A61K48/00 , C07K14/705 , C12N5/0621 , C12N2501/40 , C12N2501/415 , C12Q1/6881 , C12Q2600/158 , G01N33/56966 , G01N2333/726 , G01N2800/16
摘要： The present invention relates to a differentiation marker and a differentiation controlling technique for an eye cell. More particularly, the present invention has attained an object of providing a differentiation marker for an eye cell among the aforementioned problems, by providing a marker for identifying a cell having a high proliferation ability among corneal endothelial cells and/or the differentiation ability of a corneal endothelial cell, the marker comprising GPR49/LGR5, as well as a detection agent or detection method for identifying a cell having a high proliferation ability among corneal endothelial cells and/or the differentiation ability of a corneal endothelial cell, comprising a substance binding to GPR49/LGR5. In addition, the present invention has attained an object of providing a differentiation controlling technique for an eye cell, by providing an agent for suppressing differentiation and/or promoting proliferation of an eye cell, comprising R-spondins.
摘要翻译： 本发明涉及眼细胞的分化标记和分化控制技术。 更具体地，本发明通过提供用于鉴定角膜内皮细胞中具有高增殖能力的细胞和/或角膜分化能力的标记物，提供了上述问题中的眼细胞分化标记物的目的。 内皮细胞，包括GPR49 / LGR5的标记物，以及用于鉴定角膜内皮细胞中具有高增殖能力的细胞和/或角膜内皮细胞的分化能力的检测剂或检测方法，其包含与GPR49结合的物质 / LGR5。 此外，本发明通过提供用于抑制包含R-螺旋藻的眼细胞分化和/或促进增殖的试剂，已经达到了提供眼细胞分化控制技术的目的。
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公开(公告)号：US11633477B2
公开(公告)日：2023-04-25
申请号：US15523231
申请日：2015-10-30
申请人： KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION , THE DOSHISHA , SENJU PHARMACEUTICAL CO., LTD.
发明人： Noriko Koizumi , Naoki Okumura , Shigeru Kinoshita
IPC分类号： A61K45/00 , A61K31/4409 , A61K35/30 , A61K38/39 , A61K35/44 , A61K31/137
摘要： The present invention provides a technique for treating the cornea. More specifically, the present invention is an agent for the treatment or prevention of a state of corneal endothelial disease, the agent including at least one factor selected from the group consisting of laminin and fragments thereof, wherein the problem is solved by also providing a technique characterized in that this agent is administered together with corneal endothelial cells. Specifically, the present invention can include laminin 511 (α5β1γ1), laminin 521 (α5β2γ1), or a fragment of these.
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6.发明授权公开(公告)号：US11382904B2
公开(公告)日：2022-07-12
申请号：US15031183
申请日：2014-10-30
申请人： KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION , THE DOSHISHA , SENJU PHARMACEUTICAL CO., LTD.
发明人： Noriko Koizumi , Naoki Okumura , Shigeru Kinoshita
IPC分类号： A61K31/4439 , A61K31/444 , A61K31/4709 , A61K9/00 , A61K31/713 , A61K31/519 , A61K38/00 , A61K31/437 , A61K39/395 , C07K16/22
摘要： The present invention provides a treatment drug or prophylactic drug for diseases, disorders, or conditions related to endoplasmic reticulum (ER) stress. Specifically, the present invention provides a treatment drug or prophylactic drug for diseases, disorders, or conditions related to endoplasmic reticulum (ER) stress in the corneal epithelium, the drug containing a TGFβ-signal inhibitor. As a preferred TGFβ-signal inhibitor, the drug contains 4-[4-(1,3-benzodioxole-5-yl)-5-(2-pyridinyl)-1H-imidazole-2-yl]benzamide.
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公开(公告)号：US10959997B2
公开(公告)日：2021-03-30
申请号：US15108355
申请日：2013-12-27
申请人： KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION , THE DOSHISHA , SENJU PHARMACEUTICAL CO., LTD.
发明人： Noriko Koizumi , Naoki Okumura , Shigeru Kinoshita
IPC分类号： A61K31/4745 , A61K35/44 , A61K35/30 , A61K45/06 , A61K9/00
摘要： The present invention provides an agent for treating or preventing a disease, a disorder or a condition of a corneal endothelium, said agent comprising a corneal endothelial cell and a myosin II-specific inhibitor. More specifically, the myosin II-specific inhibitor is blebbistatin. The disease, the disorder or the condition is typically a disorder associated with Fuchs endothelial corneal dystrophy or corneal endothelial dysfunction (bullous keratopathy). Also provided is a method for treating or preventing a corneal endothelial disease, said method comprising a step of administering an effective amount of a myosin II-specific inhibitor together with a corneal endothelial cell to a subject.
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公开(公告)号：US20150044178A1
公开(公告)日：2015-02-12
申请号：US14369050
申请日：2012-12-27
申请人： Kyoto Prefectural Public University corporation , The Doshisha , Senju Pharmaceutical Co., Ltd.
发明人： Shigeru Kinoshita , Noriko Koizumi , Naoki Okumura
摘要： The present invention provides a method for the normalized culturing of corneal endothelial cells. More specifically, the present invention provides a culture-normalizing-agent of a corneal endothelial cell, comprising a fibrosis inhibitor. In detail, the present invention provides a culture-normalizing agent comprising a transforming growth factor (TGF) β signal inhibitor. The present invention also provides a culture medium for culturing a corneal endothelial cell normally, which comprises the culture-normalizing agent according to the present invention and corneal endothelium culture components. The present invention also provides a method for culturing a corneal endothelial cell normally, comprising the step of culturing a corneal endothelial cell using the culture-normalizing agent according to the present invention or the culture medium according to the present invention.
摘要翻译： 本发明提供了角膜内皮细胞的归一化培养方法。 更具体地，本发明提供了包含纤维化抑制剂的角膜内皮细胞的培养标准化剂。 详细地说，本发明提供了包含转化生长因子（TGF）和bgr的培养标准化剂。 信号抑制剂。 本发明还提供了用于培养正常角膜内皮细胞的培养基，其包含根据本发明的培养标准化剂和角膜内皮培养组分。 本发明还提供一种通常用于培养角膜内皮细胞的方法，其包括使用本发明的培养标准化剂或根据本发明的培养基培养角膜内皮细胞的步骤。
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公开(公告)号：US20210059995A1
公开(公告)日：2021-03-04
申请号：US16957757
申请日：2018-12-26
发明人： Hanako Ikeda , Akira Kakizuka , Shigeru Kinoshita , Takahiro Nakamura , Maho Nagata , Noriko Koizumi , Naoki Okumura
摘要： The inventors have found that VCP inhibitors protect corneal endothelial cells and corneal epithelial cells. Accordingly, the disclosure provides a composition for protecting a cornea comprising a compound of formula (I) or an ester, oxide, pharmaceutically acceptable salt or solvate thereof. In another aspect the disclosure provides a composition for treating and/or preventing a corneal disease comprising a compound of formula (I). In a further aspect the disclosure provides a composition for ocular irrigation comprising a compound of formula (I). In a still further aspect the disclosure provides a composition for preserving a corneal graft comprising a compound of formula (I).
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公开(公告)号：US20170202910A1
公开(公告)日：2017-07-20
申请号：US15443913
申请日：2017-02-27
申请人： Kyoto Prefectural Public University Corporation , The Doshisha , Senju Pharmaceutical Co., Ltd.
发明人： Takahiro Nakamura , Noriko Koizumi , Naoki Okumura , Shigeru Kinoshita , Kana Tominaga , Satoshi Kawasaki
IPC分类号： A61K38/17
CPC分类号： A61K38/1709 , A61K35/30 , A61K38/00 , A61K48/00 , C07K14/705 , C12N5/0621 , C12N2501/40 , C12N2501/415 , C12Q1/6881 , C12Q2600/158 , G01N33/56966 , G01N2333/726 , G01N2800/16
摘要： The present invention relates to a differentiation marker and a differentiation controlling technique for an eye cell. More particularly, the present invention has attained an object of providing a differentiation marker for an eye cell among the aforementioned problems, by providing a marker for identifying a cell having a high proliferation ability among corneal endothelial cells and/or the differentiation ability of a corneal endothelial cell, the marker comprising GPR49/LGR5, as well as a detection agent or detection method for identifying a cell having a high proliferation ability among corneal endothelial cells and/or the differentiation ability of a corneal endothelial cell, comprising a substance binding to GPR49/LGR5. In addition, the present invention has attained an object of providing a differentiation controlling technique for an eye cell, by providing an agent for suppressing differentiation and/or promoting proliferation of an eye cell, comprising R-spondins.
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