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公开(公告)号：US20230172184A1
公开(公告)日：2023-06-08
申请号：US17681854
申请日：2022-02-28
发明人： Hongwu Bai , Ying Liang , Bing Gao , Liping Bian , Aidong Sun , Xiaoming Sun , Xianjin Liu
CPC分类号： A01M7/0089 , A01B79/005
摘要： The invention discloses a traceability system for pesticide residues, the main points of the technical scheme: the traceability system for pesticide residues, comprising the following steps; step 1, collecting the basic properties of a variety of pesticides commonly used in crops; step 2, collecting the degradation properties of pesticides in many different types of soils; step 3, collecting the rainfall, irrigation conditions and other agricultural operation factors; step 4, predicting the risk of pesticide residues on the surface of the soil after use, and speculating on the transfer pollution of pesticides to crops; step 5, predicting the pesticide residues available to leach into groundwater after pesticide use; step 6, predicting the pesticide residues available to leach into surface water after pesticide use. The traceability system for pesticide residues provides a set of practical pesticide residues risk assessment tools for agricultural producers, environmental protection and legislative agencies, agricultural production management departments.
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公开(公告)号：US09745385B2
公开(公告)日：2017-08-29
申请号：US15025805
申请日：2015-01-09
发明人： Xianjin Liu , Chongxin Xu , Xiao Zhang , Yuan Liu , Yajing Xie , Cunzheng Zhang , Xiangyang Yu , Donglan Wang
CPC分类号： C07K16/4233 , A01N57/16 , A01N63/02 , C07K16/1278 , C07K2317/622 , C07K2317/76
摘要： An anthropogenic insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and a Cry1C toxin idiotype single-chain antibody encoded by said anthropogenic insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2; the antibody is a β-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV; the β-type Cry1C toxin idiotype single-chain antibody of the present invention is obtained without animal immunization, has a short preparation period and small amino acid sequence, and is suitable for large-scale in vitro production. The present invention is an entirely new insect-resistant gene resource, and has significant implications for decreasing the various safety risks associated with the widescale use of existing Bt toxins, substituting Bt toxins in the biocontrol of agricultural pests, and reducing the use of pesticides.
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3.发明授权公开(公告)号：US09725521B2
公开(公告)日：2017-08-08
申请号：US15103515
申请日：2015-01-09
发明人： Xianjin Liu , Yuan Liu , Yajing Xie , Aihua Wu , Xiao Zhang , Chongxin Xu , Yanyan Zhao , Jianfeng Zhong
CPC分类号： C07K16/4233 , A01N47/44 , A01N63/02 , C07K14/47 , C07K16/1278 , C07K2317/21 , C07K2317/622 , C07K2317/73 , C12N15/63 , C12N15/8286 , Y02A40/162
摘要： The present invention discloses a human-derived insecticidal gene and insecticidal peptide encoded by the same and application thereof. The nucleotide sequence of the human-derived insecticidal gene is as represented by SEQ ID NO.1. The amino acid sequence of the insecticidal peptide encoded by this gene is as represented by SEQ ID NO.2. The insecticidal peptide may be expressed through prokaryotic system. The primary culture has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV. It is obtained without animal immunization and has a short production cycle and a small amino acid sequence. It is suitable for in vitro mass production and may lower the safety risks resulting from wide use of existing Bt toxins and even might substitute Bt to biologically control agricultural pests in the future. It has important scientific and practical significance to reducing the use of insecticides.
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4.发明授权公开(公告)号：US11161914B2
公开(公告)日：2021-11-02
申请号：US16535296
申请日：2019-08-08
发明人： Xianjin Liu , Yajing Xie , Chongxin Xu , Xiao Zhang , Meijing Gao , Xin He , Yuan Liu , Cunzheng Zhang
摘要： The present invention provides a human-derived molecularly modified insecticidal protein, and a preparation method and application thereof, and belongs to the field of genetic engineering and biological control. The present invention provides a human-derived molecularly modified insecticidal protein, and the amino acid sequence of the insecticidal protein CCL-CCL_scFv is shown as SEQ ID No. 1. The insecticidal protein CCL-CCL_scFv shows significantly higher affinity with midgut BBMV of Plutella xylostella than Cry1Ab toxin, competes with Cry1Ab and Cry1Ac toxins for binding the midgut BBMV of Plutella xylostella, and is a mimic of Cry1Ab and Cry1Ac toxins. Through Plutella xylostella indoor insecticidal biological activity assay, the insecticidal protein shows a good insecticidal effect, and can effectively replace Cry1Ac or Cry1Ab toxin for biological control of insect pest.
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公开(公告)号：US09770038B2
公开(公告)日：2017-09-26
申请号：US15104127
申请日：2015-01-09
发明人： Xianjin Liu , Chongxin Xu , Xiao Zhang , Yuan Liu , Yajing Xie , Cunzheng Zhang , Xiangyang Yu , Donglan Wang
CPC分类号： A01N63/02 , C07K16/12 , C07K16/1278 , C07K2317/622 , C12N15/70
摘要： Provided are a human-derived insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and an anti-Cry1B toxin idiotype single-chain antibody encoded by said human-derived insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2. The idiotype single-chain antibody is a β-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV.
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公开(公告)号：US09751952B2
公开(公告)日：2017-09-05
申请号：US15104150
申请日：2015-01-09
发明人： Xianjin Liu , Chongxin Xu , Xiao Zhang , Yuan Liu , Yajing Xie , Cunzheng Zhang , Xiangyang Yu , Donglan Wang
CPC分类号： C07K16/4233 , A01N63/02 , C07K16/42 , C07K2317/14 , C07K2317/24 , C07K2317/622
摘要： An anthropogenic pest-resistant gene having a nucleotide sequence as represented by SEQ IN NO. 1, and, an anti-Cry1Ab toxin idiotypic single-chain antibody encoded by the anthropogenic pest-resistant gene and having a nucleotide sequence as represented by SEQ IN NO. 2.
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