公开(公告)号：US20070124836A1

公开(公告)日：2007-05-31

申请号：US11522307

申请日：2006-09-15

申请人： James Baum ,  Claire Cajacob ,  Pascale Feldmann ,  Gregory Heck ,  Irene Nooren ,  Geert Plaetinck ,  Wendy Maddelein ,  Ty Vaughn

发明人： James Baum ,  Claire Cajacob ,  Pascale Feldmann ,  Gregory Heck ,  Irene Nooren ,  Geert Plaetinck ,  Wendy Maddelein ,  Ty Vaughn

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8286 ,  C07H21/04 ,  C07K14/43536 ,  C07K14/43563 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8282 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

摘要： The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

摘要翻译： 本发明涉及通过抑制一种或多种生物学功能来控制害虫侵袭。 本发明提供了用于这种控制的方法和组合物。 通过将本发明提供的一种或多种重组双链RNA分子喂给害虫，通过抑制基因表达获得害虫侵染的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受虫害侵袭的转基因杀虫剂的特定组合的方法。

公开(公告)号：US20060143727A1

公开(公告)日：2006-06-29

申请号：US10545266

申请日：2004-02-17

申请人： Murtaza Alibhai ,  Claire Cajacob ,  Paul Feng ,  Gregory Heck ,  Youlin Qi ,  Stanislaw Flasinski ,  William Stallings

发明人： Murtaza Alibhai ,  Claire Cajacob ,  Paul Feng ,  Gregory Heck ,  Youlin Qi ,  Stanislaw Flasinski ,  William Stallings

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/1092 ,  C12N15/8275

摘要： The compositions and methods disclosed herein provide novel DNA molecules that encode glyphosate resistant EPSPS proteins and plants containing these new proteins. The plants that express the new EPSPS proteins are themselves tolerant to the herbicidal effects of glyphosate.

摘要翻译： 本文公开的组合物和方法提供编码抗草甘膦EPSPS蛋白质和含有这些新蛋白质的植物的新型DNA分子。 表达新EPSPS蛋白质的植物本身对草甘膦的除草效果具有耐受性。

公开(公告)号：US20070259785A1

公开(公告)日：2007-11-08

申请号：US11674005

申请日：2007-02-12

申请人： Gregory Heck ,  Tichafa Munyikwa ,  Jean Goley ,  James Roberts ,  Scott Johnson ,  Ty Vaughn

发明人： Gregory Heck ,  Tichafa Munyikwa ,  Jean Goley ,  James Roberts ,  Scott Johnson ,  Ty Vaughn

IPC分类号： A01N25/26 ,  A01H5/00 ,  C07H21/04 ,  C12N15/00 ,  C12N15/82 ,  C12N5/04 ,  C12Q1/68

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N15/8218 ,  C12N15/8286 ,  C12N2310/14 ,  C12N2320/10 ,  Y02A40/162

摘要： The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.

摘要翻译： 本发明提供了选择在目标生物体中产生dsRNA介导的基因抑制并使其能够被靶生物吸收的核苷酸序列的方法。 本发明进一步提供赋予转基因宿主细胞中此类序列稳定表达的表达构建体及其使用方法。 还提供了通过本发明的方法制备的生物，细胞和组织。

公开(公告)号：US20070136836A1

公开(公告)日：2007-06-14

申请号：US11490708

申请日：2006-07-21

申请人： Cindy Arnevik ,  Raymond Dobert ,  Qingyi Zeng ,  Jennifer Listello ,  Gregory Heck ,  John Soteres ,  Kunsheng Wu

发明人： Cindy Arnevik ,  Raymond Dobert ,  Qingyi Zeng ,  Jennifer Listello ,  Gregory Heck ,  John Soteres ,  Kunsheng Wu

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8209 ,  A01H1/02 ,  A01H1/04 ,  C12N15/8275

摘要： This invention provides a method for the development of novel plant germplasm using segregates from a transgenic line combined with PCR-based zygosity testing and, optionally, Southern blot analysis.

摘要翻译： 本发明提供了一种使用来自转基因品系的分离物与基于PCR的接合度测试和任选的Southern印迹分析结合开发新型植物种质的方法。

公开(公告)号：US20070292854A1

公开(公告)日：2007-12-20

申请号：US10919423

申请日：2004-08-16

申请人： Carl Behr ,  Gregory Heck ,  Catherine Hironaka ,  Jinsong You

发明人： Carl Behr ,  Gregory Heck ,  Catherine Hironaka ,  Jinsong You

IPC分类号： C12N15/87 ,  A01H5/00 ,  C12Q1/68 ,  C07H21/02

CPC分类号： C12N15/8275 ,  A01H5/10 ,  C12N15/8216 ,  C12N15/8221 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158

摘要： The present invention provides a DNA construct that confers tolerance to transgenic corn plant. Also provided are assays for detecting the presence of the PV-ZMGT32(nk603) corn event based on the DNA sequence of the recombinant construct inserted into the corn genome and of genomic sequences flanking the insertion site.

公开(公告)号：US20070174931A1

公开(公告)日：2007-07-26

申请号：US11725805

申请日：2007-03-20

申请人： Sherri Brown ,  Tedd Elich ,  Gregory Heck ,  Ganesh Kishore ,  Eugene Logusch ,  Sherry Logusch ,  Kenneth Piller ,  Sudabathula Rao ,  Joel Ream ,  Scott Baerson

发明人： Sherri Brown ,  Tedd Elich ,  Gregory Heck ,  Ganesh Kishore ,  Eugene Logusch ,  Sherry Logusch ,  Kenneth Piller ,  Sudabathula Rao ,  Joel Ream ,  Scott Baerson

IPC分类号： A01H1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/0071 ,  C12N9/00 ,  C12N9/0069 ,  C12N15/8222 ,  C12N15/8243 ,  C12N15/8297

摘要： Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase. 3-β hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3β-hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2β,3β-hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.

公开(公告)号：US20060191043A1

公开(公告)日：2006-08-24

申请号：US11409365

申请日：2006-04-20

申请人： Shirley Guo ,  Lisa Lee ,  Robert Harriman ,  Gregory Heck ,  Rebecca Torisky

发明人： Shirley Guo ,  Lisa Lee ,  Robert Harriman ,  Gregory Heck ,  Rebecca Torisky

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H1/00

CPC分类号： C12N15/8261 ,  C12N15/8274 ,  C12N15/8275 ,  Y02A40/146

摘要： The invention relates to DNA constructs and methods for producing transgenic glyphosate tolerant, dwarf turfgrass plants that contain these constructs. The invention also relates to the maintenance of the transgenic turfgrass stand.

摘要翻译： 本发明涉及用于产生含有这些构建体的转基因草甘膦耐受性矮小草坪草植物的DNA构建体和方法。 本发明还涉及转基因草坪草架的维持。

公开(公告)号：US20050108786A1

公开(公告)日：2005-05-19

申请号：US10911373

申请日：2004-08-04

申请人： Gregory Heck ,  Jinsong You ,  Scott Baerson

发明人： Gregory Heck ,  Jinsong You ,  Scott Baerson

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/24 ,  C12N15/82

CPC分类号： C12N15/8223 ,  C12N15/823 ,  C12N15/8275

摘要： The present invention relates to polynucleotide molecules for regulating gene expression in plants. In particular, the invention relates to promoters isolated from rice (Oryza sativa cv Nipponbare) that are useful for regulating gene expression of heterologous polynucleotide molecules in plants. The invention also relates to expression constructs and transgenic plants containing the heterologous polynucleotide molecules.

摘要翻译： 本发明涉及用于调节植物中基因表达的多核苷酸分子。 特别地，本发明涉及从水稻（Oryza sativa cv Nipponbare）中分离的启动子，其可用于调节植物中异源多核苷酸分子的基因表达。 本发明还涉及含有异源多核苷酸分子的表达构建体和转基因植物。

公开(公告)号：US20070283459A1

公开(公告)日：2007-12-06

申请号：US11239591

申请日：2005-09-30

申请人： Joseph Byrum ,  Gregory Heck ,  Thomas La Rosa

发明人： Joseph Byrum ,  Gregory Heck ,  Thomas La Rosa

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/82 ,  C07K14/415

CPC分类号： C07K14/415 ,  C12N15/8242 ,  C12N15/8261 ,  C12Q1/6895 ,  Y02A40/146

摘要： Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

摘要翻译： 公开了从大豆分离的表达序列标签（ESTs）。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 所公开的EST可用于开发用于了解关键植物发育和代谢途径的新策略。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 使用本发明提供的EST的序列同源性分析将导致对所需农学性状的更有效的基因筛选。 这些植物基因组学难题的这些精选片段的不断扩展的数据库将快速扩展后续功能验证所必需的知识，这是当前植物生物技术努力的关键限制。

公开(公告)号：US20060253933A1

公开(公告)日：2006-11-09

申请号：US11490207

申请日：2006-07-21

申请人： Sherri Brown ,  Gregory Heck ,  Jingdong Liu

发明人： Sherri Brown ,  Gregory Heck ,  Jingdong Liu

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/24 ,  C12N15/82

CPC分类号： C12Q1/6895 ,  C12N9/0069 ,  C12Q2600/158

摘要： The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize and soybean associated with the gibberellin pathway enzymes. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression and transgenic plants.

摘要翻译： 本发明在植物生物化学领域。 更具体地，本发明涉及来自植物细胞的核酸序列，特别是与赤霉素途径酶相关的玉米和大豆的核酸序列。 本发明包括编码蛋白质和蛋白质片段的核酸分子。 此外，本发明还包括如此编码的蛋白质和蛋白质的片段以及能够结合这些蛋白质或片段的抗体。 本发明还涉及使用核酸分子，蛋白质和蛋白质和抗体片段的方法，例如用于基因组图谱，基因鉴定和分析，植物育种，用于植物基因表达的构建体的制备和转基因植物。